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CRISPR Vectors
pCas-Guide-EF1a-GFP vector (with Cas9 and GFP expression) for genomic target sequence cloning (10 µg)
Function
All-in-One CRISPR/Cas9 vectors
pCAS-Scramble, pCas-Guide vector with a scrambled sequence as a negative control (10 µg)
Function
All-in-One CRISPR/Cas9 vectors
pT7-Cas9 vector in which Cas9 is under T7 for IVT to produce Cas9 mRNA
Function
CRISPR/Cas9 T7 Vectors
pT7-Guide-IVT vector for target sequence cloning; IVT to produce gRNA, (10ug)
Function
CRISPR/Cas9 T7 Vectors
AAVS1 gRNA vector, validated AAVS1 targeting sequence cloned in pCas-Guide vector
Function
All-in-One CRISPR/Cas9 vectors
Donor vector with AAVS1 homologous arms, C-terminal Myc-DDK tag, Puro resistance, ready for transgene cloning
Function
AAVS1 donor vector
p-xCas9-Guide vector (with xCas9 expression) for genomic target sequence cloning (10 µg)
Function
All-in-One CRISPR/Cas9 vectors
Vector kit for targeted transgene insertion into AAVS1 locus, including GE100023, GE100024 and GE100003
AAVS1 donor vector with AAVS1 homologous arms cloned (Puro selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
Function
AAVS1 donor vector
A scrambled sequence in pCas-Guide-EF1a-GFP vector (10 µg)
Function
All-in-One CRISPR/Cas9 vectors
pLenti-Guide-Puro, gRNA cloning vector in lenti vector with puromycin as mammalian selection marker (10ug)
Function
gRNA only vectors
AAVS1 donor vector with AAVS1 homologous arms cloned (Blasticidin selection), EF1a promoter, ready for exogenous gene cloning (10 ug)
Function
AAVS1 donor vector
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Puro resistance, ready for transgene cloning
Function
AAVS1 donor vector
AAVS1 donor vector with AAVS1 homologous arms, tGFP under EF1a promoter, and Puro selection (10 ug)
Function
AAVS1 donor vector
pCas-Guide-GFP-CRISPRa vector, All-in-one Vector for CRISPR/Cas9 Gene activation with tGFP reporter, dCas9-VP64 and gRNA cloning site.
Function
All-in-one CRISPRa SAM vector
Positive control for AAVS1 insertion (RFP insertion via CRISPR)
Function
AAVS1 donor vector
pGuide-EF1a-GFP, gRNA only vector with EF1a driven GFP
Function
gRNA only vectors
ROSA26 donor vector with ROSA26 homologous arms cloned (Puro selection), ready for exogenous gene cloning (10 ug)
Function
ROSA26 donor vector
ROSA26 donor vector with ROSA26 homologous arms, tGFP expression, and Puro selection (10 ug)
Function
ROSA26 donor vector
Donor vector with AAVS1 homologous arms, C-terminal mGFP tag, Blasticidin resistance, ready for transgene cloning
Function
AAVS1 donor vector
AAVS1 donor vector with AAVS1 homologous arms, firefly luciferase under EF1a promoter, and Puro selection (10 ug)
Function
AAVS1 donor vector
AAVS1 donor vector with AAVS1 homologous arms, tGFP under EF1a promoter, and Blasticidin selection (10 ug)
Function
AAVS1 donor vector
Vector kit for targeted transgene insertion into AAVS1 locus (EF1a, puro), including GE100023, GE100046 and GE100003. 2 vials of sequencing primers, 5' (EF51), 3' (XL39), 100 picomoles each, lyophilized
Function
CRISPR/AAVS1 kit
Vector kit for targeted transgene insertion into ROSA26 locus in mice (puro), including GE100050, GE100051 and GE100003
Function
CRISPR/ROSA26 kit
AAVS1 donor vector with AAVS1 homologous arms, tRFP under EF1a promoter, and Puro selection (10 ug)
Function
AAVS1 donor vector
