What is CRISPR?
CRISPR/Cas9, is an RNA-guided targeted genome editing tool – allows researchers to do gene knockout, knockin SNPs, insertions and deletions in cell lines and animals. The CRISPR/Cas9 genome editing system requires two components, Cas9, the endonuclease, and a guide RNA (sgRNA); sgRNA guides Cas9 to the location in the genome sequence specifically. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.
Products and Services
OriGene provides reagents to support a broad range of CRISPR/Cas9 gene editing applications. Click the following gene editing related product below and learn more about how we can help you.
Gene Knockout Kits
gRNA and donor cloning
CRISPRa / CRISPRi
AAVS1/ROSA26 Transgene Insertion
Functional CAS9 Protein
Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD,
Wu X, Jiang W, Marraffini LA, Zhang F.
Science 2013 Feb 15;339(6121):819-23
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville
JE, Church GM
Science 2013 Feb 15;339(6121):823-6
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