miRNA Validation Vectors – for the study of miRNA function

MicroRNAs (miRNAs) are important regulators for target mRNA stability and translation. Although interactions among miRNAs and their target mRNAs are critical, very little experimental data is available. Researchers have to depend on computational predictions. However, with the lack of experimental data, those computational algorithms are not always accurate.

3’UTR-luciferase reporter constructs have been used by many individual researchers as a standard method to validate interactions between miRNAs and their targets. Different from shRNA or siRNA, most of the microRNAs only lower the expression of their targets about 30%. In high over-expression systems, small effects of microRNA usually are not detectable.

OriGene has designed a couple vectors to dramatically increase the sensitivity of detection by reducing the baseline expression of luciferase reporter (100X lower than CMV promoter). This design has been validated by many scientists in their miRNA research.

MicroRNA Expression Vector    pMirTarget vector for miRNA target validation

miRNA_3_UTR workflow

SKU Description Availablility Datasheet
PR300001 Firefly luciferase assay kit, 150 assays 1 week
PR300002 Renilla luciferase assay kit, 150 assays 1 week
PR300004 5x Passive lysis buffer 1 week
PR300005 5x Firefly luciferase lysis buffer 1 week
PR300006 Firefly luciferase assay kit, 1000 assays 1 week
PR300007 Renilla luciferase assay kit, 1000 assays 1 week
PR300008 Firefly & Renilla Luciferase Single Tube Assay Kit, 150 assays 1 week
PR300009 Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 assays 1 week
PR300010 1x Passive lysis buffer 2.0 1 week