HSD17B13

HSD17B13 enzymatic activity with 75μM β-estradiol as a substrate, measured by NADH production (indicated by luminescence).

Coomassie blue staining of purified HSD17B13 protein (Cat# TP313132). The protein was produced from HEK293T cells transfected with HSD17B13 cDNA clone (Cat# RC213132) using MegaTran 2.0 (Cat# TT210002).

Western blot analysis of overexpressed human HSD17B13 lysate (5ug LY405941, LEFT) and purified recombinant protein (0.02ug TP313132, RIGHT) by using anti-HSD17B13 antibody (TA592047).

HEK293T cells were transfected with 10 different plasmids of human HSD cDNA ( HSD11B1, HSD17B2, HSD17B3, HSD17B4, HSD17B6, HSD17B7, HSD17B8, HSD17B10, HSD17B11, HSD17B13) for 48 hrs and lysed. Equivalent amounts of cell lysates (10ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSD17B13 rabbit monoclonal antibody. HSD17B13 was positive, while all others were negative(TA592047, 1：2000)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSD17B13 rabbit monoclonal antibody, TA592047(1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSD17B13 rabbit monoclonal antibody, TA592047 (1:200). *Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Data image for HSD17B13 ELISA Kit.