Genomewide siRNA coverage against human, mouse and rat
Trilencer-27 siRNA kit contains Dicer-Substrate duplexes that provide two critical improvements over the use of traditional 21mer siRNA designs. 27mer siRNA takes advantage of the natural processing by Dicer producing 10-fold higher potency and specificity than shorter 21mer RNAi forms. 27mer siRNA duplexes also evade the radar of the mammalian interferon response when expressed in mammalian cells and initiates strong and specific gene silencing.
By its optimal design, 27mer siRNA has the advantages of improved efficacy and minimal interferon response. Learn more about Dicer-Substrate technology in this Nature Publication.
Publications citing OriGene’s 27mer siRNA (view all)
Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication. Alabert, C, et al. Nature Cell Biology.
A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis. Kung, G, et al. Cell Death & Differentiation.
Features & Benefits:
- Genomewide coverage against human, mouse and rat
- 27mer Dicer-substrate duplex--higher potency & minimal interferon response
- Guaranteed gene knockdown (≥70%)
- 3 gene-specific siRNAs + 1 negative control
|SR3XXXXX||Gene-specific 3 27mer siRNA duplexes - 2 nmol each + 1 negative control - 2 nmol||$290-390||Varies|
|SRXXXXXXa/b/c||Single siRNA duplex (10nmol), buffer is included, scrambled control not included||$300||2 weeks|
|SR30002||Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol||In Stock|
|SR30003||Trilencer-27 HPRT Positive control siRNA duplex - 1 nmol||In Stock|
|SR30004||Trilencer-27 Universal scrambled negative control siRNA duplex - 2 nmol||In Stock|
|SR30005||RNAse free siRNA duplex re-suspension buffer (2ml)||In Stock|
|TT300001||SiTran1.0: Transfection reagent designed for RNAi duplex (0.5 ml)||In Stock|
siTran siRNA transfection reagent is designed to achieve high efficiency transfection for siRNA and plasmid DNA. It has been successfully used in T-cell transfection. Learn more about siTranGuarantee statement
OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).