siRNA (27 mer) Guaranteed knockdown for gene silencing

Genome wide siRNA coverage against human, mouse and rat

Trilencer-27 siRNA kit contains Dicer-Substrate duplexes that provide two critical improvements over the use of traditional 21mer siRNA designs. It takes advantage of the natural processing by Dicer producing 10-fold higher potency and specificity than shorter 21mer RNAi forms. The 27mer duplexes also evade the radar of the mammalian interferon response when expressed in mammalian cells and initiates strong and specific gene silencing.

By its optimal design, 27mer siRNA has the advantages of improved efficacy and minimal interferon response. Learn more about Dicer-Substrate technology in this Nature Publication.

Features & Benefits:

  • Genome wide coverage against human, mouse and rat
  • 27mer Dicer-substrate duplex -- higher potency & minimal interferon response
  • Guaranteed gene knockdown (≥70%)
27mer siRNA

Each of our siRNA product comes as a kit. It contains 3 unique 27mer siRNA duplexes with the size of 2 nmol each, one negative control and one buffer. Each gene specific siRNA is labeled as the kit catalog number plus a/b/c. For example,

Kit Catalog Kit Component
  • 3 gene specific siRNAs : SRxxxxxxA, SRxxxxxxB, SRxxxxxxC
  • SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmol
  • SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml
Single siRNA in Bulk

If you purchased our kits and already know which one works best for your gene, you can order this single siRNA in bulk by submitting a bulk request form. Each 10 nmol of single siRNA duplex is charged for $350. Delivery time is around 2 weeks.

You may also need the following products
SKU Description Availability Data Sheet
SR30002 Trilencer-27 Fluorescent-labeled transfection control siRNA duplex - 1 nmol In Stock
SR30003 Trilencer-27 HPRT Positive control siRNA duplex - 1 nmol In Stock
SR30004 Trilencer-27 Universal scrambled negative control siRNA duplex - 2 nmol In Stock
SR30005 RNAse free siRNA duplex re-suspension buffer (2ml) In Stock
TT320001 SiTran2.0: Transfection reagent designed for RNAi duplex (0.5 ml) In Stock

siTran siRNA transfection reagent is designed to achieve high efficiency transfection for siRNA and plasmid DNA. It has been successfully used in T-cell transfection. Learn more about siTran

Guarantee statement

OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required).

Publications citing OriGene’s 27mer siRNA (view all)

Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication. Alabert, C, et al. Nature Cell Biology.

A novel role for the apoptosis inhibitor ARC in suppressing TNFα-induced regulated necrosis. Kung, G, et al. Cell Death & Differentiation.