What is ELISA (enzyme-linked immunosorbent assay)?

Enzyme-linked immunosorbent assay, also known as Enzyme-Immunoassay (EIA) is a biochemical technique to detect and quantify the presence of antigens, hormones, and antibodies. In an ELISA, the antigen (target macromolecule) from the sample to be tested is immobilized on a solid surface (microplate) and then a matching antibody is applied which is linked to a reporter enzyme. Detection is accomplished by measuring the activity of the reporter enzyme via incubation with the appropriate substrate to produce a measurable product.

OriGene offers hundreds of ELISA kits in immunometric assay formats. These kits allow specific, quantitative measurements of disease-related proteins, including cytokines, chemokines and signaling targets.

Types of ELISA

Direct ELISA - In this method, the ELISA plate is coated with antigens, the conjugated detection antibody then binds to the target protein. OriGene produce high quality, ELISA applicable, and HRP/AP/biotin conjugated primary antibodies for you to choose to construct your assay.
Direct ELISA
Indirect ELISA - It is a two-step detection method. Similar to direct ELISA, antigen is immobilized to the plate. The primary antibody specific for the antigen binds the target, and a secondary antibody binds to the primary antibody for detection. OriGene produce high quality, ELISA applicable primary or HRP/AP/biotin conjugated secondary antibodies for you to choose to construct your assay.
Indirect ELISA
Sandwich ELISA - This method uses two types of antibodies: 1) Capture antibody 2) Detection antibody. The capture antibody is coated on the plate; this binds to the antigen which in turn binds to the primary antibody for detection. OriGene offer sandwich ELISA kits in either conventional or Luminex assay platform for wide-ranged selected targets to meet your research purpose.
Sandwich ELISA

ELISA Kits by Research Area

Disease/Research Area Targeted Molecules
InflammationIL6, Calprotectin
Acute Kidney InjuryNGAL
Liver DiseasesHSD17B13
Blood clottingD-Dimer
Multiple SclerosisGFAP
Cardiovascular DiseasesST2

Recent Citations using our ELISA Kits

1. Influence of Lactobacillus paracasei HII01 Supplementation on Glycemia and Inflammatory Biomarkers in Type 2 Diabetes: A Randomized Clinical Trial

Published in Foods (Basel, Switzerland) 2021 using EA101010
PubMed: 34201653

2. Linking the IL-17A immune response with NMR-based faecal metabolic profile in IBD patients treated with Mastiha

Published in Biomedicine & Pharmacotherapy 2021 using EA100036

3. Evaluation of matrix metalloproteinase-9 plasma levels in untreated new Relapsing-remitting multiple sclerosis patients and their first-degree family

Published in Metabolic brain disease 2021 using EA100106
PubMed: 34115275

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