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Applications: FC, IHC, WB Reactivities: Human
Applications: FC, IF, IHC, IP, WB Reactivities: Human, Mouse, Rat
Applications: IHC, WB Reactivities: Human
Applications: ELISA, FACS, FC, IF, IHC, IP, WB
Applications: WB
Applications: IF, IP, WB
Expression Host: HEK293T Purity:> 80%
Expression Host: HEK293T Purity: > 80%
Reactivity: Human Sensitivity: 0.1 ng/ml
Reactivity: Human Sensitivity: 33.5 pg/ml
Target: Lentiviral p24 protein
Target: AAV particles
Target: Packaging plasmids and transfection reagen
Fluorescent Label: mGFP Particles: >10^7 TU/m
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![Immunohistochemical staining of paraffin-embedded testis using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 ([B21-100]) at 110C for 10 min, anti-MAGE-A4 diluted to 1:50. Detection was done with Polink1 Broad Mouse and Rabbit C/N [D11-18] with DAB Kit. Image 40x magnification.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/100/ta505362-101-h.jpg)
![Immunohistochemical staining of paraffin-embedded bladder cancer using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 ([B21-100]) at 110C for 10 min, anti-MAGE-A4 diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N [D11-18] with DAB Kit. Image 40x magnification.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/100/ta505362-102-h.jpg)
![Immunohistochemical staining of paraffin-embedded MAGE-A4 cDNA expressing CytoSections ([TS418952]) using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 ([B21-100]) at 110C for 10 min, anti-MAGE-A4 diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N [D11-18] with DAB Kit. Image 40x magnification.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/100/ta505362-103-h.jpg)
![Immunohistochemical staining of paraffin-embedded negative control CytoSections ([TC400001]) using anti-MAGE-A4 C/N TA505362 clone OTI1F9 mouse monoclonal antibody. HIER TEE buffer pH9 ([B21-100]) at 110C for 10 min, anti-MAGE-A4 diluted to 1:100. Detection was done with Polink1 Broad Mouse and Rabbit C/N [D11-18] with DAB Kit. Negative control [TC400001] shows no staining as predicted. Image 40x magnification.](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/100/ta505362-104-h.jpg)
![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY MAGEA4 (Cat# [RC223991], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4(Cat# TA505362). Positive lysates [LY423276] (100ug) and [LC423276] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta505362-1-w.jpg)
![HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY LGR5 (Cat# [RC212825], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5(Cat# TA503316). Positive lysates [LY401213] (100ug) and [LC401213] (20ug) can be purchased separately from OriGene.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta503316-1-w.jpg)
![Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5 ([RC212825]).](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta503316-1-f.jpg)
![HEK293T cells transfected with either [RC212825] overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGR5 antibody (TA503316), and then analyzed by flow cytometry.](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta503316-1-c.jpg)
![Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <a href="https://www.ncbi.nlm.nih.gov/pubmed/27398792" target="_blank">View Citation</a><br />Pubmed id: 27398792<br />Memo: Human; IF](https://cdn.origene.com/catalog/product/assets/images/antibody/100/ta503316-202-f.jpg)
![Immunohistology staining on IL6 overexpressed cytosection [TS402078P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC202078], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ta592569-1-h.jpg)
![Immunohistology staining on SPON2 overexpressed cytosection [TS406764P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC206764], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts406764p5-100.jpg)
![Immunohistology staining on CH25H overexpressed cytosection [TS404716P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC204716], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts404716p5-100.jpg)
![Immunohistology staining on CLDN22 overexpressed cytosection [TS425263P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC225263], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts425263p5-100.jpg)
![Immunohistology staining on PCDHA11 overexpressed cytosection [TS421707P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC221707], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts421707p5-100.jpg)
![Immunohistology staining on IL1B overexpressed cytosection [TS402079P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC202079], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts402079p5-100.jpg)
![Immunohistology staining on IL10 overexpressed cytosection [TS416785P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC216785], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts416785p5-100.jpg)
![Immunohistology staining on IL11 overexpressed cytosection [TS404493P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC204493], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts404493p5-100.jpg)
![Immunohistology staining on TNF overexpressed cytosection [TS406983P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC206983], 5 micron sections, 40x magnification](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ts406983p5-100.jpg)
![Immunofluorescent staining of 293T cells transfected by human CTAG1B plasmid([RC213318]) using anti-DDK antibody (TA592569/green, right), compared to an Isotype control(Left).(1:400)](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ta592569-1-f.jpg)
![Immunoprecipitation (IP) of DDK by using DDK Rabbit monoclonal antibody TA592569. Rabbit IgG control serves as the negative control. 293T cells were transfected with C-terminal DDK-tagged OTUB2 overexpression plasmid. 1ml overexpression cell lysates were first precleared with agarose beads for 1h. Then precleared lysates were incubated with beads crosslinked with antibody for 2h. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with anti-DDK mouse monoclonal antibody([TA180144], 1:1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/primary-antibody/100/ta592569-1-p.jpg)
![Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid ([RC210768]) (Red) using an anti-DDK antibody (TA592569), compared to an isotype control (green) and a PBS control (blue)(1:100).](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ta592569-1-c.jpg)
![Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid ([RC210768]) (Red) using an anti-DDK antibody (TA592569), compared to an isotype control (green), and a PBS control (blue)(1:100).](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/100/ta592569-1.jpg)
![Cos7 cells transiently transfected with turboGFP tagged BID ORF cDNA clone ([RC100007]) for mitochondria marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/ta150041-1-f.jpg)
![Cos7 cells transiently transfected with turboGFP tagged LAMP1 ORF cDNA clone ([RC100016]) for lysosome marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/antitag/ta150041-2-f.jpg)
![Coomassie blue staining of purified IFIH1 protein (Cat# TP315661). The protein was produced from HEK293T cells transfected with IFIH1 cDNA clone (Cat# [RC215661]) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp315661.jpg)
![Coomassie blue staining of purified PKP2 protein (Cat #TP310514). The protein was produced from mammalian cells transfected with PKP2 cDNA clone (Cat #[RC210514]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp310514.jpg)
![Coomassie blue staining of purified HSD17B13 protein (Cat# TP313132). The protein was produced from HEK293T cells transfected with HSD17B13 cDNA clone (Cat# [RC213132]) using MegaTran 2.0 (Cat# [TT210002]).](https://cdn.origene.com/catalog/product/assets/images/proteins/tp313132.jpg)
