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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAGEA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505362)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY MAGEA4 (Cat# [RC223991], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEA4(Cat# TA505362). Positive lysates [LY423276] (100ug) and [LC423276] (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-MAGEA4 monoclonal antibody (1:500).

Flow cytometric analysis of fixed and permeabilized NCI-H1299 cells, using anti-MAGEA4 antibody (TA505362, Red), compared to an isotype control (green) (1:100).

Flow cytometric analysis of living A375 cells, using anti-MAGEA4 antibody (TA505362, Red), compared to an isotype control (green) (1:100).

MAGEA4 Mouse Monoclonal Antibody

Applications: FC, IHC, WB Reactivities: Human

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 <a href="http://www.ncbi.nlm.nih.gov/pubmed/25060070" target="_blank">View Citation</a>

Figure from citation: Immunohistochemical analysis of Lgr5 expression in human normal colon biopsies; Lgr5+ cells are localized at the crypt base, where bona fide stem-like cells home; magnification is indicated in the boxes. Dilution: 1:200 <a href="http://www.ncbi.nlm.nih.gov/pubmed/22927961" target="_blank">View Citation</a>

Figure from citation: Immunohistochemical staining of Lgr5 in normal gastric tissues. The normal gastric tissue, 40×magnified (A) and 400× magnified (B). Yellowspots represent the Lgr5 positive cells that are localized only at base of the glands in normal gastric mucosa. The arrows in the pictures show the immunoreactive cells stained in yellow color. <a href="http://www.ncbi.nlm.nih.gov/pubmed/23664892" target="_blank">View Citation</a>

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503316)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY LGR5 (Cat# [RC212825], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5(Cat# TA503316). Positive lysates [LY401213] (100ug) and [LC401213] (20ug) can be purchased separately from OriGene.

Immunoprecipitation (IP) of LGR5 by using TrueMab monoclonal anti-LGR5 antibodies (Negative control: IP without adding anti-LGR5 antibody.). For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Anti-LGR5 mouse monoclonal antibody (TA503316) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGR5 ([RC212825]).

Figure from citation: Immunocytochemistry of epithelium stem cell phenotype. Isolated dental epithelium following 5-day culture, overwhelmingly expressed Lgr5. Dilution: 1:100 <a href="http://www.ncbi.nlm.nih.gov/pubmed/24345734" target="_blank">View Citation</a>

Immunofluorescent staining of HT29 cells using anti-LGR5 mouse monoclonal antibody (TA503316, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

HEK293T cells transfected with either [RC212825] overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGR5 antibody (TA503316), and then analyzed by flow cytometry.

Figure from citation: Cytofluorimetric analysis of Lgr5 expression by PKH high cells over 4 weeks of culture. Data are expressed as percent mean values (6 SD) of 5 consecutive experiments. Dilution: 1:400 <a href="http://www.ncbi.nlm.nih.gov/pubmed/22927961" target="_blank">View Citation</a>

Figure from citation: Flow cytometry of the SB cells. The G4 region was further divided into P1 and P2. Lgr5, a stem cell marker, was expressed by 32% of the P2 population. Black: staining with Lgr5; red: staining with the isotype control. <a href="http://www.ncbi.nlm.nih.gov/pubmed/24465489" target="_blank">View Citation</a>

Figure from citation: [Nature] Cells expressing the stem cell marker Lgr5 was detected by immunostaining of hCECs in 2D culture. <a href="https://www.ncbi.nlm.nih.gov/pubmed/27398792" target="_blank">View Citation</a><br />Pubmed id: 27398792<br />Memo: Human; IF

LGR5 Mouse Monoclonal Antibody

Applications: FC, IF, IHC, IP, WB Reactivities: Human, Mouse, Rat

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MCAMMCAM monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-MCAMMCAM monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MCAM mouse monoclonal antibody. (UM800051; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human melanoma using anti-CD146 (MCAM) clone UMAB154 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800051 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110°C for 3min using pressure chamber/cooker. The tumor cells shows strong membraneous and cytoplasmic staining.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Western blot analysis of HEK293 cell lysate (35ug) by using anti-MCAM monoclonal antibody.

Western blot analysis of Hela cell lysate (35ug) by using anti-MCAM monoclonal antibody.

Western blot analysis of MALME3M cell lysate (35ug) by using anti-MCAM monoclonal antibody.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

CD146 (MCAM) Mouse Monoclonal Antibody

Applications: IHC, WB Reactivities: Human

Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with TA592569 at 1:3000.

Immunoblot analysis of 5 different N-terminal DDK tagged overexpression lysates with TA592569 at 1:3000. 1. Trim29 fragment （1-163aa） 2. SNCA 3. SNCB 4. TNFA 5. Trim29 fragment (164aa-449aa)

Immunoblot analysis of picked 6 different DDK tagged overexpression lysates with TA592569 at 1:3000.(Lane 1/2 N-terminal DDK tag;Lane 3/4 C-terminal 3XDDK tag; Lane 5/6 N-terminal 3XDDK tag.)

Immunohistology staining on IL6 overexpressed cytosection [TS402078P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC202078], 5 micron sections, 40x magnification

Immunohistology staining on SPON2 overexpressed cytosection [TS406764P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC206764], 5 micron sections, 40x magnification

Immunohistology staining on CH25H overexpressed cytosection [TS404716P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC204716], 5 micron sections, 40x magnification

Immunohistology staining on CLDN22 overexpressed cytosection [TS425263P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC225263], 5 micron sections, 40x magnification

Immunohistology staining on PCDHA11 overexpressed cytosection [TS421707P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC221707], 5 micron sections, 40x magnification

Immunohistology staining on IL1B overexpressed cytosection [TS402079P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC202079], 5 micron sections, 40x magnification

Immunohistology staining on IL10 overexpressed cytosection [TS416785P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC216785], 5 micron sections, 40x magnification

Immunohistology staining on IL11 overexpressed cytosection [TS404493P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC204493], 5 micron sections, 40x magnification

Immunohistology staining on TNF overexpressed cytosection [TS406983P5] with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit ([D11-18]). Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone [RC206983], 5 micron sections, 40x magnification

Immunofluorescent staining of 293T cells transfected by human CTAG1B plasmid([RC213318]) using anti-DDK antibody (TA592569/green, right), compared to an Isotype control(Left).(1:400)

Immunoprecipitation (IP) of DDK by using DDK Rabbit monoclonal antibody TA592569. Rabbit IgG control serves as the negative control. 293T cells were transfected with C-terminal DDK-tagged OTUB2 overexpression plasmid. 1ml overexpression cell lysates were first precleared with agarose beads for 1h. Then precleared lysates were incubated with beads crosslinked with antibody for 2h. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with anti-DDK mouse monoclonal antibody([TA180144], 1:1000).

Immunoprecipitation (IP) of DDK by using DDK Rabbit monoclonal antibody TA592569. Rabbit IgG control serves as the negative control. 293T cells were transfected with N-terminal DDK-tagged Trim29 overexpression plasmid. 1ml overexpression cell lysates were first precleared with agarose beads for 1h. Then precleared lysates were incubated with beads crosslinked with antibody for 2h. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with anti-DDK mouse monoclonal antibody(TA50011, 1:1000).

Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid ([RC210768]) (Red) using an anti-DDK antibody (TA592569), compared to an isotype control (green) and a PBS control (blue)(1:100).

Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid ([RC210768]) (Red) using an anti-DDK antibody (TA592569), compared to an isotype control (green), and a PBS control (blue)(1:100).

Anti-DDK Rabbit Monoclonal Antibody

Applications: ELISA, FACS, FC, IF, IHC, IP, WB

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY tdTomato (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tdTomato (TA180009, 1:2000).

tdTomato Mouse Monoclonal Antibody

Applications: WB

Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.

Figure from citation: Western blot analysis of tGFP fused protein level by using anti-tGFP antibody in 293T cells. <a href="http://www.ncbi.nlm.nih.gov/pubmed/25525876" target="_blank">View Citation</a>

Figure from citation: Western Blot of tGFP-fused protein level by using anti-tGFP antibody in 293A cells. Dilution: 1:1000 <a href="http://www.ncbi.nlm.nih.gov/pubmed/23318425" target="_blank">View Citation</a>

Cos7 cells transiently transfected with turboGFP tagged BID ORF cDNA clone ([RC100007]) for mitochondria marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).

Cos7 cells transiently transfected with turboGFP tagged LAMP1 ORF cDNA clone ([RC100016]) for lysosome marking were immuno-stained with OriGene 2H8 anti-turboGFP antibody (TA150041, 1:100), and then stained red with an Alexa-568 conjugated secondary antibody (1:1000).

Figure from citation: Immunofluorescence of tGFP-fused protein level by using anti-tGFP antibody in mouse E18 hippocampal pyramidal neurons. Dilution: 1:250 <a href="http://www.ncbi.nlm.nih.gov/pubmed/24444792" target="_blank">View Citation</a>

Immunoprecipitation (IP) of recombinant tGFP fusion protein from overexpression lysate made with HEK293T cells transfected by plasmid expressing tGFP-DDK. The immunoprecipitated tGFP fusion protein was analyzed by immunoblot with rabbit anti-DDK polyclonal antibody. (Left lane: Negative control, IP without adding TA150041)

Figure from citation: ChIP of tGFP-fused protein level by using anti-tGFP antibody in human Panc1 cells. <a href="http://www.ncbi.nlm.nih.gov/pubmed/23801748" target="_blank">View Citation</a>

TurboGFP Mouse Monoclonal Antibody

Applications: IF, IP, WB

Comparison of each of the chemokine levels (CCL2, CXCL11, CXCL9, CCL17, CXCL10) between anti-MDA5 antibody-positive and -negative patients. Anti-MDA5 antibody status was determined by ELISA using recombinant MDA5/IFIH1 antigen (OriGene TP315661). The Mann-Whitney U-test estimated the P value. * P < 0.05. Figure cited from Sci Rep, PMID: 28487565

Coomassie blue staining of purified IFIH1 protein (Cat# TP315661). The protein was produced from HEK293T cells transfected with IFIH1 cDNA clone (Cat# [RC215661]) using MegaTran 2.0 (Cat# [TT210002]).

MDA5 (IFIH1) Human Recombinant Protein

Expression Host: HEK293T Purity:> 80%

Coomassie blue staining of purified PKP2 protein (Cat #TP310514). The protein was produced from mammalian cells transfected with PKP2 cDNA clone (Cat #[RC210514]).

Plakophilin 2 (PKP2) Human Recombinant Protein

Expression Host: HEK293T Purity: > 80%

HSD17B13 enzymatic activity with 75μM β-estradiol as a substrate, measured by NADH production (indicated by luminescence).

Coomassie blue staining of purified HSD17B13 protein (Cat# TP313132). The protein was produced from HEK293T cells transfected with HSD17B13 cDNA clone (Cat# [RC213132]) using MegaTran 2.0 (Cat# [TT210002]).

HSD17B13 Human Recombinant Protein

Expression Host: HEK293T Purity: > 80%

Data image for HSD17B13 ELISA Kit.

Human HSD17B13 ELISA KIT

Reactivity: Human Sensitivity: 0.1 ng/ml

MAGEA4 ELISA Kit data image.

Human MAGEA4 ELISA Kit

Reactivity: Human Sensitivity: 33.5 pg/ml

Lenti RapidTiter Sticks Reference Card

Lenti RapidTiter Sticks

Target: Lentiviral p24 protein

AAV qPCR fast Titer Kit

Target: AAV particles

Lentiviral Packaging Kits

Target: Packaging plasmids and transfection reagen

Lentiviral Control Particles

Fluorescent Label: mGFP Particles: >10^7 TU/m

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