Alpha-Gold Competent Cells

OriGene’s choice for in-house transformation of DNA…now available to our customers

Alpha-Gold has been used for cloning & transformation of OriGene’s major plasmid products such as TrueORF Gold cDNA clones, HuSH-29 shRNA & MicroRNA expression plasmids.

Features:

  • Both Electroporation Grade and Chemically Competent
  • Comparable to DH5άTM in transformation efficiency
  • Accommodates small and larger plasmids
  • recA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk -, mk +) supE44 thi-1 phoA Δ(lacZYA argF)U169 Φ80lacZΔM15 λ-

DNA Alpha-Gold Efficiency
pUC19 - 2.7Kb 1.8 x 109 cfu/ug
8.2Kb plasmid 3.8 x 108 cfu/ug
13.3Kb plasmid 3.0 x 108 cfu/ug
50Kb cosmid 3.8 x 107 cfu/ug


Kit components:

  • Alpha-Gold competent cells
  • Positive Control DNA - pUC19 (10pg/μl)
  • SOC medium – 10ml

Applications:

  • lacZ marker for blue/white color screening
  • Transformation of cloned DNA into bacterial cells
  • Ideal for subcloning and generating cDNA libraries

Transformation Protocol
Competent Cells SDS

SKU Description Data sheet
CC100001 Alpha-Gold competent cells (20 x 50 ul)
CC100002 Alpha-Gold competent cells (5 x 200 ul)