DDKTrap™ Anti-DDK Affinity Resin

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DDKTrap™ Anti-DDK Affinity Resin is a cost-effective, high-performance solution for purifying DDK or FLAG® or DYKDDDDK-tagged proteins. With exceptional binding capacity (1 mg protein/mL resin), it ensures efficient and reliable purification of DDK-tagged proteins, including those expressed from OriGene’s TrueORF® clones and overexpression lysates.

Comparison with ANTI‐FLAG® M2 Affinity Gel

860 ug purification elution from 1 mL resin using DDKTrap™ compared to 310 ug purification from the M2 using the same lysate prepared from the transfected cell.

 

 

 

860 ug purification elution from 1 mL resin using DDKTrap™ compared to 310 ug purification from the M2 using the same lysate prepared from the transfected cell.

 

In this study, DDKTrap™ showed 2.7X higher protein yield than M2 Affinity Gel

Key Features:

  • High Binding Capacity: >1 mg DDK-tagged protein/mL resin.
  • TrueORF®-Compatible: Seamless purification of proteins expressed from OriGene TrueORF clones.
  • Flexible Compatibility: Purify N- and C-terminal DDK tagged protein.
  • Economical and Reusable: Retains binding efficiency for up to 3 purification cycles.*
  • Trusted by OriGene: Used in the purification of MVPro recombinant proteins.

Product Use Case Examples:

Purify proteins with different MW

High level of protein purifications

Purify proteins with different MW

High level of protein purifications

SKU Description Add to Cart
AR100028S DDKTrap Anti-DDK Affinity Resin, 1 mL
AR100028M DDKTrap Anti-DDK Affinity Resin, 5 mL
AR100028L DDKTrap Anti-DDK Affinity Resin, 10 mL

For a limited time, the 1 mL size of DDKTrap Anti-DDK Affinity Resin is available at a 25% new product introduction discount.

Product Resources:

About DDK tag

The DDK or DYKDDDDK or FLAG® tag is a small, hydrophilic peptide engineered for efficient protein purification and detection. Composed of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp Asp-Lys, abbreviated as DDK) with a molecular weight of about 1 kDa, it is also known as the DDK tag. Ideally positioned on the surface of the protein of interest (such as on the N or C terminus), the tag allows for straightforward purification and detection by binding readily to the anti-DDK antibodies which are conjugated to the resin.

FLAG is a registered trademark of Sigma-Aldrich Co. LLC.
*Resin reuse depends on the intrinsic properties of the protein and the elution method employed. To prevent cross-contamination, the same resin should not be used for purifying multiple proteins.