DDKTrap™ Anti-DDK Affinity Resin

DDKTrap™ Anti-DDK Affinity Resin is a cost-effective, high-performance solution for purifying DDK or FLAG® or DYKDDDDK-tagged proteins. With exceptional binding capacity (1 mg protein/mL resin), it ensures efficient and reliable purification of DDK-tagged proteins, including those expressed from OriGene’s TrueORF® clones and overexpression lysates.
Comparison with ANTI‐FLAG® M2 Affinity Gel
860 ug purification elution from 1 mL resin using DDKTrap™ compared to 310 ug purification from the M2 using the same lysate prepared from the transfected cell.
In this study, DDKTrap™ showed 2.7X higher protein yield than M2 Affinity Gel
Key Features:
- High Binding Capacity: >1 mg DDK-tagged protein/mL resin.
- TrueORF®-Compatible: Seamless purification of proteins expressed from OriGene TrueORF clones.
- Flexible Compatibility: Purify N- and C-terminal DDK tagged protein.
- Economical and Reusable: Retains binding efficiency for up to 3 purification cycles.*
- Trusted by OriGene: Used in the purification of MVPro recombinant proteins.
Product Use Case Examples:
Purify proteins with different MW
High level of protein purifications
SKU | Description | Add to Cart |
---|---|---|
DDKTrap Anti-DDK Affinity Resin, 1 mL | ||
DDKTrap Anti-DDK Affinity Resin, 5 mL | ||
DDKTrap Anti-DDK Affinity Resin, 10 mL |
For a limited time, the 1 mL size of DDKTrap Anti-DDK Affinity Resin is available at a 25% new product introduction discount.
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About DDK tag
The DDK or DYKDDDDK or FLAG® tag is a small, hydrophilic peptide engineered for efficient protein purification and detection. Composed of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp Asp-Lys, abbreviated as DDK) with a molecular weight of about 1 kDa, it is also known as the DDK tag. Ideally positioned on the surface of the protein of interest (such as on the N or C terminus), the tag allows for straightforward purification and detection by binding readily to the anti-DDK antibodies which are conjugated to the resin.
FLAG is a registered trademark of Sigma-Aldrich Co. LLC.
*Resin
reuse depends on the intrinsic properties of the protein and the
elution method employed. To prevent cross-contamination, the same
resin should not be used for purifying multiple proteins.