OriGene employs CRISPR to validate antibody specificity
Why KO validation?
Specificity has been a major unsolved issue for antibody application. OriGene has been a pioneer in providing highly validated antibodies. Using KO lysate is OriGene’s second initiative after UltraMAB, which utilize high-density protein arrays (17K protein chip) to validated antibody specificity.
Western blot using knockout (KO) cell lysate has become a new practice for antibody validation. In KO validation, a knockout cell line was paired with the parental cell line in western blot analysis. A truly specific antibody recognizes the target protein of right molecular weight in the WT cell lysate, but not in the KO cell lysate.