AAV Products and Services
Adeno-associated virus (AAV) is a small, non-enveloped virus with a single stranded DNA genome. This virus is commonly used as an efficient vehicle to mediate DNA delivery into mammalian cells, both in vitro and in vivo. The benefits of using AAV for research purposes rank from tissue sensitivity, high transduction efficiency, stable gene expression and low immunogenicity.
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Solutions for entire AAV workflow
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Cotransfection
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AAV Particles Ready for Transduction
Downstream Applications
AAV Serotype Selection Guide
One of the useful features of AAV is its serotypes, which defines the preference of the types of cells/organs they infect. The tables below provide the selection guide for in vitro and in vivo applications.
| Cell Line | AAV-1 | AAV-2 | AAV-3 | AAV-4 | AAV-5 | AAV-6 | AAV-8 | AAV-9 | AAV-DJ | AAV-DJ/8 |
|---|---|---|---|---|---|---|---|---|---|---|
| Huh-7 | 13 | 100 | 2.5 | 0.0 | 0.1 | 10 | 0.7 | 0.0 | 500 | 0.2 |
| HEK293 | 25 | 100 | 2.5 | 0.1 | 0.1 | 5 | 0.7 | 0.1 | 500 | 0.3 |
| HeLa | 3 | 100 | 2.0 | 0.1 | 6.7 | 1 | 0.2 | 0.1 | 667 | 0.2 |
| HepG2 | 3 | 100 | 16.7 | 0.3 | 1.7 | 5 | 0.3 | ND | 1250 | 0.5 |
| Hep1A | 20 | 100 | 0.2 | 1.0 | 0.1 | 1 | 0.2 | 0.0 | 400 | 0.1 |
| 911 | 17 | 100 | 11 | 0.2 | 0.1 | 17 | 0.1 | ND | 500 | 0.0 |
| CHO | 100 | 100 | 14 | 1.4 | 333 | 50 | 10 | 1.0 | 25000 | 5.0 |
| COS | 33 | 100 | 33 | 3.3 | 5.0 | 14 | 2.0 | 0.5 | 500 | 0.3 |
| MeWo | 10 | 100 | 20 | 0.3 | 6.7 | 10 | 1.0 | 0.2 | 2857 | 1.0 |
| NIH3T3 | 10 | 100 | 2.9 | 2.9 | 0.3 | 10 | 0.3 | ND | 500 | 0.1 |
| A549 | 14 | 100 | 20 | ND | 0.5 | 10 | 0.5 | 0.1 | 1000 | 0.1 |
| HT1180 | 20 | 100 | 10 | 0.1 | 0.3 | 33 | 0.5 | 0.1 | 333 | 0.2 |
| Monocytes | 1111 | 100 | ND | ND | 125 | 1429 | ND | ND | 100 | ND |
| Immature DC | 2500 | 100 | ND | ND | 222 | 2857 | ND | ND | 200 | ND |
| Mature DC | 2222 | 100 | ND | ND | 333 | 3333 | ND | ND | 100 | ND |
Note: Infectivity rates normalized to AAV-2 = 100. ND = Not Determined.
Grimm D, Lee JS, Wang L, et al. In vitro and in vivo gene therapy vector evolution via multispecies interbreeding and retargeting of adeno-associated viruses. J virol. 2008;82(12):5887-5911. doi:10.1128/JVI.00254-08