SPR Services
Accelerate Discovery with Precision Kinetics
OriGene offers Surface Plasmon Resonance (SPR) analysis services to help you characterize molecular interactions with unparalleled sensitivity and accuracy. Whether you're investigating antibody-antigen binding, small molecule-target affinity, or protein-protein interactions, SPR delivers real-time kinetic data in a label-free environment — empowering confident decision-making throughout your R&D pipeline.
SPR Services Advantages
- Technology:
- We utilize a well-proven Biacore X100 platform for real-time and label-free detection
- Flexible Sample Support:
- Compatible with peptides, proteins, and antibodies
- Experienced Scientific Support:
- Expert support from experimental design to data interpretation
SPR Service Applications:
- Antibody Binding Analysis
- Kinetics
- Affinity
SPR Workflow:
Timeline: 2-3 Days for 5 sample pairs (antibodies and proteins) after sample submission
Deliverables:
- Sensorgrams of all interactions
- Calculated kinetic constants (KD, Kon, Koff)
- Fitting models and residual analysis
- Experimental conditions and assay parameters
- An optional consultation to interpret and apply the findings
Sample Requirements:
| Type | Amount | Concentration |
|---|---|---|
| Antibody | At least 250 uL | 0.1-1 ug/mL, usually 0.5 ug/mL |
| Protein | At least 500 uL | At least 1uM |
Case Studies:
1. SPR Characterization and Assay Validation of SYP Antibody
- The SYP antibody (rabbit) captured on a Protein A chip demonstrates binding to the SYP protein.
- SPR analysis determined an affinity constant of 2.469x10-10 M for the antibody.
- The quality control report confirms that the assay passed all required parameters.
2. SPR Analysis and Assay Validation of Anti-FLAG M2 Antibody
- Anti-FLAG M2 antibody (mouse) immobilized on a CM5 chip shows binding to STING protein (antigen).
- SPR analysis determined an affinity constant of 1.623 × 10⁻⁷ M for the antibody.
- The quality control report confirms that the assay met all required criteria.
3. SPR Analysis and Assay Validation of Three Antibody A Clones
- Sensograms showing affinity and kinetic profiles of antibody A clones 1A1, 2A3, and 3B4.
- SPR analysis determined the affinity constants to be 1.08x10-10 M for 1A1, 7.2x10-11 M for 2A3, and 2.6x10-10 M for 3B4.
- Quality control report confirms that each assay met all required validation criteria.
