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Control Solutions for Every Experiment

Don't risk misinterpreting your results—controls are essential

Skipping controls can lead to misleading conclusions and wasted experiments. Our validated controls give you the confidence that your data is accurate and reproducible.
OriGene's comprehensive range of scientifically validated controls for Western Blot, ELISA, qPCR, IHC, ICC, IF, ISH, and AAV/Lenti controls for viral gene transfer applications.

Western Blot Controls

Comprehensive control solutions ensuring reliable and reproducible Western Blot results

Loading Control Antibodies

Validated antibodies for protein normalization

Positive Controls

Over-expression cell lysates for validation

Negative Controls

Knockdown cell lysates for specificity testing

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Immunoblot analysis for validation of anti-PRLR antibody using PRLR over-expression lysate (LY424444). Ref
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Western blot analysis of extracts from 6 different cell lines by using anti-GAPDH monoclonal antibody (TA802519).
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Western blot analysis of EGFR expression in WT and EGFR Knockdown HeLa cell lysates (LY300151) using anti-EGFR antibody.

ELISA Controls

Standarized controls for accurate and precise ELISA assay performance

Standards

Full-length recombinant proteins for standard curves

Positive Controls

Over-expression lysates for assay validation
Human PD-1 recombinant protein (TP310364) was used as a calibrator standard to measure PD-1 concentration on the surface of isolated EVs. Ref
Human VAMP-2 overexpression lysate (LY415423) was used to screen and validate monoclonal antibodies for the development of a novel digital immunoassay (SIMOA) to measure VAMP-2 levels in cerebrospinal fluid in this study. Ref
Human PLIN1 recombinant protein (TP306292) was used as an antigen to coat ELISA plates for detecting autoantibodies against PLIN1. Ref
Human ATF4 recombinant protein (TP760367) was used as a calibrator standard in MSD’s ECL-based immunoassay to measure elevated ATF4 protein levels. Ref

qPCR Controls

Reliable primer sets and template standard for housekeeping genes ensuring accurate quantitative PCR results

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Housekeeping Gene Primers

Validated primer sets for multiple reference genes including GAPDH, β-actin, and HPRT1

  • Multiple reference gene options
  • Validated across multiple cell types
  • Optimized primer efficiency

 

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qPCR Template Standards

Over 18,000 gene specific qPCR template standards for human or mouse transcripts

  • As a positive control in all qPCR methods (probe and SYBR Green)
  • Determine the absolute transcript copy number of an experiment sample using the standard curve method
  • Generate a standard curve to determine PCR efficiency in a comparative Ct method
Human β-Actin primers (HP204660) were used as an internal reference in RT-qPCR analyses to assess gene expression changes following PGC-1α silencing in bladder cancer cells. Ref
Human TNFAIP2 qPCR Primer Pair (HP209262) and Human GAPDH qPCR Primer Pair (HP205798) were used to measure gene expression levels, helping to assess the impact of TNFAIP2 knockout on cisplatin resistance in laryngeal cancer cells. Ref
Mouse qPCR Primer Pairs for Cxcr4 (MP202423), Cxcr5 (MP202369) , Ccr7 (MP202402), and Gapdh (MP205604) were used in this study to quantify gene expression changes in B cells, helping to elucidate the molecular mechanisms by which C. difficile toxin B impairs immune memory responses. Ref
miR-199a1 and AXL mRNA expression in cord blood samples were quantified using OriGene’s qStar miRNA detection system with qStar primer pairs and corresponding copy number standard (HK300226, HK201002). Ref

IHC, ICC, IF & ISH Controls

Specialized controls for immunohistochemistry, immunocytochemistry, immunofluorescence, and in situ hybridization

CytoSections

FFPE sections of transiently transfected cDNA-specific gene tragets expressed in human HEK293T cells - control alternatives for IHC, ICC, IF, and ISH.
RNA expression is preserved alongside protein expression in CytoSections, making them ideal as positive and negative controls for in situ hybridization (ISH) applications. Positive staining on ENPP1 CytoSections (TS409222P5) using an ENPP1 RNA probe, while no staining is observed with the same probe on ENPP2 CytoSections (TS405377P5), confirming specificity.
IHC staining of (left) MAGEA3 CytoSection (TS403288P5) using anti-MAGEA3 antibody (TA800804) and (right) untransfected HEK293T Cytosection (TC400001) using anti-MAGEA3 antibody (TA800804).
CytoSections serve as effective immunofluorescence (IF) controls for validating antibody specificity. Alexa Fluor 488-conjugated anti-MAGEA4 antibody is used to detect MAGEA4 in MAGEA4 CytoSections (TS418952P5), while showing no cross-reactivity in MAGEA3 CytoSections (TS403288P5).

Viral Controls

Control particles for AAV and Lentiviral-medicated gene delivery applications

AAV Control Particles

Ultra-purified, iodixanol-gradient-purified viral tools with guaranteed high titer (>1013 GC/mL), designed for reliable transduction into dividing and non-dividing cells in both in vitro and in vivo applications.

Lentiviral Control Particles

Engineered with a robust >1x107 TU/mL titer and fluorescent or selectable markers offer researchers reliable, easy-to-quantify negative controls for optimizing transduction efficiency across diverse cell lines.
HEK 293T cells infected by AAV9 viral particles for 72 hours. AAV9 with CMV promoter-driven expression of GFP, >10^13 GC/mL (CV900007S).
AAV-PHP.eB with CMV promoter-driven expression of GFP, >10^13 GC/mL (CV900012S).
HEK293 cells (96-well plate) were transduced with different volumes of control Lenti particles, scrambled shRNA, expressing GFP and Puro, >1x10^7 TU/ml (TR30021V). Fluorescence pictures taken 72 hrs. after.
HEK293 cells (96-well plate) were transduced with different volumes of control Lenti particles, scrambled shRNA, expressing GFP and Puro, >1x10^7 TU/ml (TR30033V). Fluorescence pictures taken 72 hrs. after.