Comprehensive EV Solutions to Accelerate Your Exosome Research
Isolation | Detection | Cargo Analysis | Functional Assays
What are Extracellular Vesicles (EVs)?
Extracellular vesicles, including exosomes (30–150 nm in size), are secreted by cells into biofluids such as blood, cerebrospinal fluid, and urine. These vesicles carry proteins, RNA, and DNA and are emerging as powerful biomarkers and therapeutic delivery tools.
New! MageExo EV Isolation Kits
A rapid, lipid nanoprobe–based EV isolation solution enabling direct, high-efficiency capture from plasma and serum in under 30 minutes with >90% efficiency to support robust EV-based multi-omics workflows.
New! Immunocapture EV Isolation Kits
Enable isolation of CD9, CD81 EV population from various biofluids, cell culture media with >90% capture efficiency.
EV Research Product Solutions
Isolation
- Lipid Nanoprobe Capture
- Total EV isolation from plasma, serum
- Precipitation Method
- Polymer-based
- Immunocapture Method
- High-affinity antibody coupled to magnetic beads
Detection
- EV Markers
- Antibodies against general EV markers
- Full-length recombinant EV marker proteins for use as a standard in immunoassays
- ELISA Kit for detection of EV markers
Cargo Analysis
- miRNA
- qPCR miRNA detection system
- Disease Markers
- Antibody against EV enriched biomarkers
Functional Analysis
- GFP-Tag clone
- Florescent EV markers for analysis
- miRNA Validation Vectors
- Over-expression and miRNA target vectors of your choice
EV markers Detection
Antibodies
Accurate EV characterization starts with the right tools. OriGene offers high-quality antibodies against key extracellular vesicle markers, validated for applications including Western blotting (WB), immunohistochemistry (IHC), Flow Cytometry (FC), and Simple Western (SW).
| Marker Category | Marker | Function in Exosome Biology | Applications | SKU | Cart |
|---|---|---|---|---|---|
| Multi-pass transmembrane proteins | CD9 | Exosomal membrane protein; involved in vesicle fusion | FC | ||
| CD63 | Common exosomal marker; involved in trafficking | IF, IHC | |||
| CD81 | Vesicle biogenesis and membrane organization | ELISA, FC, IF, IHC, IP, WB | |||
| EpCAM | Often enriched in tumor-derived exosomes | IF, IHC, WB | |||
| Single-pass transmembrane protein | EMPRINN | Cell adhesion, signaling, and matrix remodeling; enriched in tumor-derived exosomes | FC, IF, IHC, WB | ||
| TSG101 | Involved in MVB formation and exosome biogenesis | IF, IHC, IP, WB | |||
| Membrane protein binding | ALIX | Membrane remodeling, endosomal sorting, and vesicle budding | IHC, WB | ||
| Syntenin | Plays role in cargo loading via ESCRT-independent pathways | IHC, WB | |||
| Cytosolic chaperones | HSP70 | Stress-induced proteins enriched in exosome | FC, IF, IHC, WB | ||
| HSP90 | Stress-induced proteins enriched in exosome | WB | |||
| Lipid protein binding | Flotillin-1 | Assist membrane scaffolding, sorting in exosomes | FC, ICC, WB | ||
| Flotillin-2 | Assist membrane scaffolding, sorting in exosomes | IP, WB | |||
| Adhesion proteins | Annexin A2 | Involved in membrane trafficking and vesicle fusion | FC, IF, IHC, WB | ||
| MFGE8 | Mediator of exosome binding and uptake; immune modulator | IHC, WB | |||
| Regulators negative marker | Rab27a | Regulate MVB docking and exosome secretion | WB | ||
| Calnexin | ER marker, low abundance in pure exosome preps | FC, IF, IHC, IP, SW, WB |
Western blot analysis of EV proteins alix, β‐actin, syntenin (), CD81, annexin A1, and TSG101. Ref#1
Simple Western analysis of EV lysates (0.2 mg/mL) from HEK293T cell culture using CD63 mouse mAb ().
Western blot analysis of EVspecific markers, including CD81 (), in NK-exosomes. Ref#2
Recombinant Proteins
OriGene offers HEK293-expressed full-length EV marker recombinant proteins that have been cited in numerous publications for use as a standard in immunoassays for EV quantification.
| Target | SKUs | Cart |
|---|---|---|
| CD9 | ||
| CD63 | ||
| CD81 |
CD81 () recombinant proteins were used as standards in the Simoa assay to measure the levels of CD63 and CD81 in EV samples isolated from plasma. Ref#4
CD63 () and CD81 () recombinant proteins were used as standards in the Simoa assay to measure the levels of CD63 and CD81 in EV samples isolated from plasma. Ref#3
ELISA Kit
Human Exosome ELISA Kits allow direct EV quantification from native biofluids without the need for isolation, minimizing EV loss. They also offer the advantages of high sensitivity and reduced sample volume requirements.
| Target | SKUs | Cart |
|---|---|---|
| CD9 | ||
| CD81 |
Kit Features
- Detection: CD9 or CD81-positive exosome
- Sample: Cell Culture, Serum Plasma
- Avg Spike Recovery: Cell Culture (95%), Plasma (109%), Serum (96%)
- Avg Dilution Linearity: Cell Culture (104%), Plasma (97%), Serum (98%)
- Specificity: Intact Exosomes
- Precision: Intra-assay (<6%) and Inter-assay (<4%)
- Sensitivity (LLOD): 1.91E+06 particles/mL (≈ 13.2 ng/mL total exosome protein equivalence)
- Assay Range: 4.53E+6 - 2.9E+8 particles/mL (31.25 ng/ml-2ug/ml total exosome protein equivalence)
miRNA and Protein Cargo Profiling in EVs
EV cargo detection leverages qPCR-based miRNA assays and antibody-based protein detection to identify disease biomarkers within EVs, enabling precise and non-invasive analysis of disease-related molecular signatures.
miRNA qPCR Detection Assays
OriGene’s primer-based, SYBR Green qPCR microRNA detection system not only offers researchers a fast and simple method for profiling miRNA expression levels, but also provides means to quantify the results down to the absolute copy number of miRNA.
Learn more here
Antibodies against Diseases related Biomarkers
Exosomes and other (EVs) play a vital role in intercellular communication and are increasingly recognized as key contributors to disease progression. From cancer to neurodegenerative disorders such as Alzheimer’s and Parkinson’s disease, EVs serve as carriers of functional biomolecules, transferring cargo both locally and systemically through the blood, lymph, and cerebrospinal fluid (CSF).
OriGene supports exosome and EV research with a growing portfolio of high-quality antibodies targeting EV-associated biomarkers
| Disease Area | EVs or Exosomes Biomarkers |
|---|---|
| Cancer | AHSG, CAV1, CA125, CD39, CD73, EGFR, Galectin-9, GPC1, HSP70, KRAS, MET, PD-L1, Survivin, S100A9, TGF-B, TACSTD2, VIM |
| Metabolic | Adiponectin, Calpain-2 |
| Neurodegenerative | Amyloid, Tau/MAPT, pTau(217), TDP43, SYP, SYT4, SNAP-25, GAP43, L1CAM, SNCA, KRT8, NRGN, PARK7, NEFL |
| Inflammation | APMAP, WDR1, CD25, CD54, CD14, TNF-a, HMGB1 |
WB analysis of overexpressed and endogenous hu, ms, and rt TDP43 Mouse MAb ()
IF staining of Human CD274 in COS7 cells Mouse MAb ()
IHC staining of VIM in Human Appendix Rabbit MAb ( )
IHC staining of NEFL in Human Embryonic brain Mouse MAb ()
IHC staining of KRAS in Human Colon Mouse MAb ()
IHC staining of Adiponectin in Human Kidney Mouse MAb ()
Visualize and Track EV Biology in Real Time
EV or exosomes functional analysis using GFP-tagged EV marker cDNA clones and GFP-tagged miRNA plasmids enables visualization and tracking of exosome biogenesis, cargo loading, and cellular uptake, providing insights into intercellular communication mechanisms. These tools facilitate real-time monitoring of miRNA delivery and functional impact in recipient cells.
GFP-Tagged EV Marker Clones
Markers relevant to EV are fused with a GFP protein, allowing for direct visualization of the EV or its structures without further manipulation. Our expression-ready GFP-tagged ORF clones are ready for transfection.
Quantification of EV-derived CD9-GFP () signal in the nucleoplasm of recipient SW480 cells. Ref#6
Fluorescence micrographs of primary cultured astrocytes transfected with tGFP-CD9 () plasmid (green), treated with fluorescent Aβ1-42 (red), and immunolabeled for ASM (blue), showing colocalization between the three channels in the perinuclear area (merge). Ref#5
Exosome quantification using MACSQuant analysis indicated that KTZ (1 µM) significantly suppressed exosome secretion in 786-O-CD63 GFP (). Ref#7
miRNA Validation Vectors
Explore our genome-wide miRNA coverage for human, mouse, and rat models across key disease areas. Ideal for transfection monitoring and miRNA target validation. Learn more here
| Disease Area | EV miRNA Cargo |
|---|---|
| Cancer | miR-106a , miR-194, miR-193a, miR-126, miR-148a, miR-196b, miR-378, miR-382, miR-96 |
| Diabetes | miR-10b, miR-133b, miR-192, miR-194, miR-215, miR-218, miR-23a, miR-30a, miR-34a |
| Neurodegenerative | miR-135a, miR-19b, miR-195, miR-24 |
| Inflammation | miR-21, miR-146a, miR-155, miR-223, miR-124 |
A2780 cells were stably transfected with pCMV-miR21 (), and miR-21 expression was quantified by qRT-PCR. Ref#8
miR-214 overexpression induced the proinflammatory activation of RAW 264.7 macrophages. Realtime qPCR of IL-1β, IL-6, and TNF-α mRNA in the control and miR-214-overexpressing RAW 264.7 macrophages Ref#9
A2780 cells were stably transfected with pCMV-miR21 (), and miR-21 expression was quantified by qRT-PCR. Ref#8
miR-214 overexpression induced the proinflammatory activation of RAW 264.7 macrophages. Realtime qPCR of IL-1β, IL-6, and TNF-α mRNA in the control and miR-214-overexpressing RAW 264.7 macrophages Ref#9
References
- Bonner SE, van, Phillips W, et al. Scalable purification of extracellular vesicles with high yield and purity using multimodal flow through chromatography. Journal of Extracellular Biology. 2024;3(2). doi: 10.1002/jex2.138
- Lee J, Lee SA, Gu NY, et al. Canine Natural Killer Cell-Derived Exosomes Exhibit Antitumor Activity in a Mouse Model of Canine Mammary Tumor. Biomed Res Int. 2021;2021:6690704. Published 2021 Sep 4. doi: 10.1155/2021/6690704
- Ter-Ovanesyan D, Gilboa T, Budnik B, et al. Improved isolation of extracellular vesicles by removal of both free proteins and lipoproteins. Rodrigues ML, Pfeffer SR, Byrd J, Zhou Q, eds. eLife. 2023;12:e86394. doi: 10.7554/eLife.86394
- Lee EE, Winston-Gray C, Barlow JW, Rissman RA, Jeste DV. Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology. Frontiers in Psychiatry. 2021;11. doi: 10.3389/fpsyt.2020.532624
- Elsherbini A, Zhu Z, Quadri Z, et al. Novel Isolation Method Reveals Sex-Specific Composition and Neurotoxicity of Small Extracellular Vesicles in a Mouse Model of Alzheimer's Disease. Cells. 2023;12(12):1623. Published 2023 Jun 14. doi: 10.3390/cells12121623
- Santos MF, Rappa G, Fontana S, et al. Anti-Human CD9 Fab Fragment Antibody Blocks the Extracellular Vesicle-Mediated Increase in Malignancy of Colon Cancer Cells. Cells. 2022;11(16):2474. Published 2022 Aug 10. doi: 10.3390/cells11162474
- Greenberg JW, Kim H, Moustafa AA, et al. Repurposing ketoconazole as an exosome directed adjunct to sunitinib in treating renal cell carcinoma. Sci Rep. 2021;11(1):10200. Published 2021 May 13. doi: 10.1038/s41598-021-89655-w
- Upregulation of miR-21 in Cisplatin Resistant Ovarian Cancer via JNK-1/c-Jun Pathway. PLoS ONE. 2014;9(12):e116447-e116447. doi: 10.1371/journal.pone.0116447
- Andreucci E, Ruzzolini J, Bianchini F, et al. miR-214-Enriched Extracellular Vesicles Released by Acid-Adapted Melanoma Cells Promote Inflammatory Macrophage-Dependent Tumor Trans-Endothelial Migration. Cancers (Basel). 2022;14(20):5090. Published 2022 Oct 18. doi: 10.3390/cancers14205090
