ORF Cloning Vectors

The same ORF insert in multiple context for diverse applications

All TrueORF inserts are housed in a pCMV6-Entry vector and can be easily shuttled via PrecisonShuttle, a simple 'cut-and-paste' procedure, into any of the destination vectors. With the wide selection of destination vectors, TrueORF clones are ready-to-use for many downstream protein studies.

The destination vectors offers choices of:

  • Epitope tags for protein purification detection
  • Fluorescent protein tags for protein visualization
  • Various drug marker for stable line establishment
  • E.coli expression vectors

Options of purchasing the vectors or requesting custom shuttling are available.

TrueORF Utility
Entry Vector SKU C-Tag E. coli
Selection 		Cell
Selection 		Expression
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug PS100001 Myc-DDK Kanamycin Neomycin Mammalian
pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug PS100063 Myc-DDK Kanamycin Neomycin Mammalian

The vectors could be ordered as:

Lentiviral Vectors

OriGene now offers multiple Lentiviral vectors. Easy shuttling of TrueORF insert. Click the Lentiviral tab below

Epitope tagging

Destination Vector1 SKU C-Tag N-Tag E. coli
Selection 		Cell
Selection 		Expression
pCMV6-AN-3HA PS100066   3 HA Ampicillin Neomycin Mammalian
pCMV6-AN-IT5 PS100095   IT5 Ampicillin Neomycin Mammalian
pCMV6-AN-IT6 PS100096   IT6 Ampicillin Neomycin Mammalian
pCMV6-AN-IT10 PS100097   IT10 Ampicillin Neomycin Mammalian
pLenti-N-Myc-DDK-IRES-Puro PS100105   Myc-DDK Chloramphenicol Puromycin Mammalian
pCMV6-AN-FC PS100055   FC Ampicillin Neomycin Mammalian
pCMV6-AN-FC-S PS100056   FC Ampicillin Neomycin Mammalian
pCMV6-AN-3DDK PS100058   3DDK Ampicillin Neomycin Mammalian
pCMV6-AN-His PS100011   His Ampicillin Neomycin Mammalian
pCMV6-AN-Myc PS100012   Myc Ampicillin Neomycin Mammalian
pCMV6-AN-HA PS100013   HA Ampicillin Neomycin Mammalian
pCMV6-AN-DDK PS100014   DDK Ampicillin Neomycin Mammalian
pCMV6-AN-His-Myc PS100015   His-Myc Ampicillin Neomycin Mammalian
pCMV6-AN-Myc-DDK PS100016   Myc-DDK Ampicillin Neomycin Mammalian
pCMV6-AN-His-HA PS100017   His-HA Ampicillin Neomycin Mammalian
pCMV6-AN-His-DDK PS100018   His-DDK Ampicillin Neomycin Mammalian
pCMV6-AC PS100020     Ampicillin Neomycin Mammalian
pCMV6-AC-3HA PS100067 3 HA   Ampicillin Neomycin Mammalian
pCMV6-AC-3DDK PS100057 3DDK   Ampicillin Neomycin Mammalian
pCMV6-AC-DDK PS100005 DDK   Ampicillin Neomycin Mammalian
pCMV6-AC-DDK-His PS100009 DDK-His   Ampicillin Neomycin Mammalian
pCMV6-XL5-DDK-His PS100068 DDK-His   Ampicillin   Mammalian
pCMV6-AC-FC PS100053 FC   Ampicillin Neomycin Mammalian
pCMV6-AC-FC-S PS100054 FC   Ampicillin Neomycin Mammalian
plenti-C-HA-IRES-BSD PS100104 HA   Chloramphenicol Blasticidin Mammalian
pCMV6-AC-HA PS100004 HA   Ampicillin Neomycin Mammalian
pLenti-C-HA-DDK-P2A-Puro PS100101 HA-DDK   Chloramphenicol Puromycin Mammalian
pCMV6-AC-HA-His PS100008 HA-His   Ampicillin Neomycin Mammalian
pCMV6-AN-GFP-C-His PS100061 His tGFP Ampicillin Neomycin Mammalian
pCMV6-AC-His PS100002 His   Ampicillin Neomycin Mammalian
pCMV6-AC-IT10 PS100100 IT10   Ampicillin Neomycin Mammalian
pCMV6-AC-IT5 PS100098 IT5   Ampicillin Neomycin Mammalian
pCMV6-AC-IT6 PS100099 IT6   Ampicillin Neomycin Mammalian
pCMV6-AC-Myc PS100003 Myc   Ampicillin Neomycin Mammalian
pCMV6-AC-Myc-DDK PS100007 Myc-DDK   Ampicillin Neomycin Mammalian
pLenti-C-Myc-DDK PS100064 Myc-DDK   Chloramphenicol   Mammalian
pLenti-C-Myc-DDK-IRES-Puro PS100069 Myc-DDK   Chloramphenicol Puromycin Mammalian
pLenti-C-Myc-DDK-P2A-Puro PS100092 Myc-DDK   Chloramphenicol Puromycin Mammalian
pLenti-EF1a-C-Myc-DDK-IRES-Puro      PS100085 Myc-DDK   Chloramphenicol Puromycin Mammalian
pLenti-C-Myc-DDK-P2A-tGFP PS100088 Myc-DDK   Chloramphenicol   Mammalian
pAAV-AC-Myc-DDK PS100089 Myc-DDK   ampicilin   Mammalian
pLenti-C-Myc-DDK-IRES-Neo PS100081 Myc-DDK   Chloramphenicol Neomycin Mammalian
pLenti-C-Myc-DDK-IRES-BSD PS100082 Myc-DDK   Chloramphenicol Blasticidin Mammalian
pLenti-C-Myc-DDK-P2A-BSD PS100103 Myc-DDK   Chloramphenicol Blasticidin Mammalian
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro PS100106 Myc-DDK HA Chloramphenicol Puromycin Mammalian
pCMV6-AC-Myc-His PS100006 Myc-His   Ampicillin Neomycin Mammalian
pAAVS1-Puro-DNR GE100024 Myc-DDK   Ampicillin Puromycin Mammalian

FP tagging

Destination Vector2 SKU FP Feature C-Tag N-Tag E. coli Selection Cell Selection
pLenti-N-tRFP PS100077 Red, dimer   tRFP Chloramphenicol  
pLenti-N-tYFP PS100078 Yellow, dimer   tYFP Chloramphenicol  
pLenti-N-tGFP PS100073 Green, dimer   tGFP Chloramphenicol  
pCMV6-AC-IRES-GFP-Puro PS100059 Green, dimer     Ampicillin Puromycin
pCMV6-AN-FP602 PS100045 Red, dimer   TurboFP602 Ampicillin Neomycin
pCMV6-AN-FP635 PS100046 Far-red, dimer   TurboFP635 Ampicillin Neomycin
pCMV6-AN-mKate PS100047 Far-red, monomer   mKate Ampicillin Neomycin
pCMV6-AN-mGFP PS100048 Green, monomer   mGFP Ampicillin Neomycin
pCMV6-AN-mRFP PS100049 Red, monomer   mRFP Ampicillin Neomycin
pCMV6-AN-mYFP PS100050 Yellow, monomer   mYFP Ampicillin Neomycin
pCMV6-AN-mBFP PS100051 Blue, monomer   mBFP Ampicillin Neomycin
pCMV6-AN-mCFP PS100052 Cyan, monomer   mCFP Ampicillin Neomycin
pCMV6-AN-RFP PS100033 Red,dimer   TurboRFP Ampicillin Neomycin
pCMV6-AN-YFP PS100035 Yellow, dimer   TurboYFP Ampicillin Neomycin
pCMV6-AN-GFP PS100019 Green, dimer   TurboGFP Ampicillin Neomycin
pCMV6-A-GFP PS100026 Green, dimer     Ampicillin  
pLenti-C-GFP PS100065 Green, dimer GFP   Chloramphenicol  
pCMV6-AN-GFP-C-His PS100061 Green, dimer His tGFP Ampicillin Neomycin
pCMV6-AC-mBFP PS100043 Blue, monomer mBFP   Ampicillin Neomycin
pCMV6-AC-mCFP PS100044 Cyan, monomer mCFP   Ampicillin Neomycin
pCMV6-AC-mGFP PS100040 Green, monomer mGFP   Ampicillin Neomycin
pLenti-C-mGFP PS100071 Green, Monomer mGFP   Chloramphenicol  
pLenti-C-mGFP-P2A-Puro PS100093 Green, Monomer mGFP   Chloramphenicol Puromycin
pLenti-EF1a-C-mGFP PS100084 Green, Monomer mGFP   Chloramphenicol  
pLenti-C-mGFP-P2A-BSD PS100102 Green, monomer mGFP   Chloramphenicol Blasticidin
pCMV6-AC-mKate PS100039 Far-red, monomer mKate   Ampicillin Neomycin
pCMV6-AC-mRFP PS100041 Red, monomer mRFP   Ampicillin Neomycin
pLenti-mRFP-P2A-Puro PS100094 Puromycin mRFP   Chloramphenicol Puromycin
pCMV6-AC-IRES-GFP PS100027 Green, dimer Myc-DDK   Ampicillin Neomycin
pLenti-C-Myc-DDK-P2A-tGFP PS100088 GFP, dimer Myc-DDK   Chloramphenicol  
pCMV6-AC-mYFP PS100042 Yellow, monomer mYFP   Ampicillin Neomycin
pLenti-EF1a-C-tGFP PS100072 Green, dimer tGFP   Chloramphenicol  
pAAV-AC-GFP PS100090 GFP, dimer tGFP   ampicilin  
pAAV-AC-RFP PS100091 RFP, dimer tRFP   ampicilin  
pLenti-C-tRFP PS100074 Red, dimer tRFP   Chloramphenicol  
pCMV6-AC-FP602 PS100037 Red, dimer TurboFP602   Ampicillin Neomycin
pCMV6-AC-FP635 PS100038 Far-red, dimer TurboFP635   Ampicillin Neomycin
pCMV6-AC-GFP PS100010 Green, dimer TurboGFP   Ampicillin Neomycin
pCMV6-AC-RFP PS100034 Red,dimer TurboRFP   Ampicillin Neomycin
pCMV6-AC-YFP PS100036 Yellow, dimer TurboYFP   Ampicillin Neomycin
pLenti-C-tYFP PS100079 Yellow, dimer tYFP   Chloramphenicol  
Photo gallery of the fluorescent-tagged proteins generated by OriGene with TrueORF clones

Selection markers

Destination Vector3 SKU C-Tag E. coli
Selection 		Cell
Selection 		Expression
pLenti-N-Myc-DDK-IRES-Puro PS100105   Chloramphenicol Puromycin Mammalian
pCMV6-AC-IRES-GFP-Puro PS100059   Ampicillin Puromycin Mammalian
pCMV6-AC PS100020   Ampicillin Neomycin Mammalian
pCMV6-A-BSD PS100022   Ampicillin Blasticidin Mammalian
pCMV6-A-EM7-BSD PS100023   Ampicillin/Blasticidin Blasticidin Mammalian
pCMV6-A-Hygro PS100024   Ampicillin Hygromycin Mammalian
pCMV6-A-Puro PS100025   Ampicillin Puromycin Mammalian
pCMV6-A-GFP PS100026   Ampicillin   Mammalian
plenti-C-HA-IRES-BSD PS100104 HA Chloramphenicol Blasticidin Mammalian
pLenti-C-HA-DDK-P2A-Puro PS100101 HA-DDK Chloramphenicol Puromycin Mammalian
pLenti-C-mGFP-P2A-Puro PS100093 mGFP Chloramphenicol Puromycin Mammalian
pLenti-C-mGFP-P2A-BSD PS100102 mGFP Chloramphenicol Blasticidin Mammalian
pLenti-mRFP-P2A-Puro PS100094 mRFP Chloramphenicol Puromycin Mammalian
pCMV6-AC-IRES-GFP PS100027 Myc-DDK Ampicillin Neomycin Mammalian
pLenti-C-Myc-DDK-IRES-Puro PS100069 Myc-DDK Chloramphenicol Puromycin Mammalian
pLenti-C-Myc-DDK-P2A-Puro PS100092 Myc-DDK Chloramphenicol Puromycin Mammalian
pLenti-EF1a-C-Myc-DDK-IRES-Puro      PS100085 Myc-DDK Chloramphenicol Puromycin Mammalian
pLenti-C-Myc-DDK-IRES-Neo PS100081 Myc-DDK Chloramphenicol Neomycin Mammalian
pLenti-C-Myc-DDK-IRES-BSD PS100082 Myc-DDK Chloramphenicol Blasticidin Mammalian
pLenti-C-Myc-DDK-P2A-BSD PS100103 Myc-DDK Chloramphenicol Blasticidin Mammalian
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro PS100106 Myc-DDK Chloramphenicol Puromycin Mammalian

Lenti / AAV

Destination Vector4 SKU C-Tag N-Tag E. coli
Selection 		Cell Selection
pLenti-P2A-tGFP PS100108     Chloramphenicol tGFP
pLenti-P2A-Puro PS100109     Chloramphenicol Puromycin
pLenti-N-tRFP PS100077   tRFP Chloramphenicol  
pLenti-N-tYFP PS100078   tYFP Chloramphenicol  
pLenti-N-Myc-DDK-IRES-Puro PS100105   Myc-DDK Chloramphenicol Puromycin
pLenti-N-tGFP PS100073   tGFP Chloramphenicol  
pLenti-C-mCFP-P2A-BSD PS100107 mCFP   Chloramphenicol Blasticidin
pLenti-C-GFP PS100065 GFP   Chloramphenicol  
plenti-C-HA-IRES-BSD PS100104 HA   Chloramphenicol Blasticidin
pLenti-C-HA-DDK-P2A-Puro PS100101 HA-DDK   Chloramphenicol Puromycin
pLenti-C-mGFP PS100071 mGFP   Chloramphenicol  
pLenti-C-mGFP-P2A-Puro PS100093 mGFP   Chloramphenicol Puromycin
pLenti-EF1a-C-mGFP PS100084 mGFP   Chloramphenicol  
pLenti-C-mGFP-P2A-BSD PS100102 mGFP   Chloramphenicol Blasticidin
pLenti-mRFP-P2A-Puro PS100094 mRFP   Chloramphenicol Puromycin
pLenti-C-Myc-DDK PS100064 Myc-DDK   Chloramphenicol  
pLenti-C-Myc-DDK-IRES-Puro PS100069 Myc-DDK   Chloramphenicol Puromycin
pLenti-C-Myc-DDK-P2A-Puro PS100092 Myc-DDK   Chloramphenicol Puromycin
pLenti-EF1a-C-Myc-DDK-IRES-Puro      PS100085 Myc-DDK   Chloramphenicol Puromycin
pLenti-C-Myc-DDK-P2A-tGFP PS100088 Myc-DDK   Chloramphenicol  
pAAV-AC-Myc-DDK PS100089 Myc-DDK   ampicilin  
pLenti-C-Myc-DDK-IRES-Neo PS100081 Myc-DDK   Chloramphenicol Neomycin
pLenti-C-Myc-DDK-IRES-BSD PS100082 Myc-DDK   Chloramphenicol Blasticidin
pLenti-C-Myc-DDK-P2A-BSD PS100103 Myc-DDK   Chloramphenicol Blasticidin
pLenti-EF1a-N-HA-C-Myc-DDK-IRES-Puro PS100106 Myc-DDK HA Chloramphenicol Puromycin
pLenti-EF1a-C-tGFP PS100072 tGFP   Chloramphenicol  
pAAV-AC-GFP PS100090 tGFP   ampicilin  
pAAV-AC-RFP PS100091 tRFP   ampicilin  
pLenti-C-tRFP PS100074 tRFP   Chloramphenicol  
pLenti-C-tYFP PS100079 tYFP   Chloramphenicol  

Bacterial expression

Destination Vector5 SKU C-Tag N-Tag E. coli
Selection 		Expression
pEX-1 PS100075     Ampicillin E. coli
pEX-N-His-GST PS100028   His-GST Ampicillin E. coli
pEX-N-GST PS100029   GST Ampicillin E. coli
pEX-N-His PS100030   His Ampicillin E. coli
pEX-C-GST PS100083 GST   Ampicillin E. coli
pEX-C-His PS100076 His   Ampicillin E. coli
The new E.coli destination vectors expand the utility of 37,000 TrueORF clones for protein production in bacteria.
Expression Validation Product Manual

Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich

Vector Resources