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ORF Cloning Vectors
The same ORF insert in multiple context for diverse applications
All TrueORF inserts are housed in a pCMV6-Entry vector and can be easily shuttled via PrecisonShuttle, a simple 'cut-and-paste' procedure, into any of
the destination vectors. With the wide selection of destination vectors, TrueORF clones are ready-to-use for many
downstream protein studies.
The destination vectors offers choices of:
- Epitope tags for protein purification detection
- Fluorescent protein tags for protein visualization
- Various drug marker for stable line establishment
- E.coli expression vectors
Options of purchasing the vectors or requesting custom shuttling are available.
| Entry Vector | SKU | C-Tag | E. coli Selection |
Cell Selection |
Expression |
|---|---|---|---|---|---|
| pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug | PS100001 | Myc-DDK | Kanamycin | Neomycin | Mammalian |
| pCMV6-Entry2, mammalian vector with C-terminal Myc-DDK tag, 10ug | PS100063 | Myc-DDK | Kanamycin | Neomycin | Mammalian |
The vectors could be ordered as:
- Stand-alone products
- As custom shuttling service where OriGene subclones the ORF insert of your interest from the Entry vector into any of these destination vectors.
- Stable Cell Line Development Services
Lentiviral Vectors
OriGene now offers multiple Lentiviral vectors. Easy shuttling of TrueORF insert. Click the Lentiviral tab below
Epitope tagging
FP tagging
Photo gallery of the fluorescent-tagged proteins generated by OriGene with TrueORF clones
Selection markers
Lenti / AAV
Transgene Insertion via CRISPR
Bacterial expression
| The new E.coli destination vectors expand the utility of 37,000 TrueORF clones for protein production in bacteria. | |
| Expression Validation | Product Manual |
Peptide sequence of the DDK-tag (Flag®): N-DYKDDDDK-C
Flag® is a registered trademark of Sigma-Aldrich