Luciferase Assays for firefly and Renilla luciferase

Luciferase assays are often used for the studies of different biological activities. It can quantitively measure the bioactivities using bioluminescent light output. OriGene offers a full range of luciferase assay products and tools for your research.

Our Luciferase assay kits include Firefly, Renilla and dual luciferases kits, which measure bioactivities of different luciferase reporters. We also have a luciferase reporter vector (Cat# PR100001) for the study of promoter function, and many 3’UTR clones which use luciferase signals to investigate miRNA regulation of gene expression.

Firefly or Renilla Single Assay Kit

Our Firefly (Cat# PR300001) and Renilla (Cat# PR300002) kits offer high sensitivity and linearity comparable to leading competitors. Unlike other kits that have limited shelf life after reagents are reconstituted, our kits provide substrate separately from reaction buffer, for consistent performance over time. These are flash-type assays that require that samples be read in a luminometer immediately after adding reagents.

Firefly & Renilla Dual Assay Kit

Renilla luciferase is commonly used as a normalizing transfection control for Firefly reporter assays. Our Firefly & Renilla Single Tube Assay (Cat# PR300008) allows you to sequentially measure Firefly and Renilla activities in the same sample. Our assay offers sensitivity and linearity comparable to other two-reporter assays. This assay is a flash-type assay, which requires signal to be read in a luminometer immediately after adding reagents.

Features

  1. High sensitivity and linearity, with low cost
  2. Add substrates to buffers as needed for consistent performance
  3. Firefly assay available in lyophilized format
  4. Water-soluble Renilla substrate

Comparison of OriGene’s & Promega’s dual luciferase assays

Luciferase reactions were performed according to manufacturers instructions using titration curves of purified firefly (Promega) or Renilla luciferase (Ray Biotech). Luciferase enzymes were diluted in 1X passive lysis buffer from the assay kit tested. Background from reagents without enzyme added was subtracted from luminescence values. Both assay systems showed comparable sensitivity and dynamic range.

Features

  1. Assay Firefly & Renilla in the same sample
  2. Excellent performance with low cost
  3. Add substrates to buffers as needed for consistent performance
  4. Water-soluble Renilla substrate

Overview of dual luciferase assay



Firefly & Renilla single tube assay in transfected cells

Example of Firefly & Renilla Luciferase Single Tube Assay using HeLa cells. Cells transfected with firefly luciferase alone (firefly only), firefly + Renilla, or no transfection (no tfx). Quenched firefly is the residual firefly signal after adding Renilla working solution to firefly-only transfected cells.
SKU Description Availablility Datasheet
PR300001 Firefly luciferase assay kit, 150 assays 1 week
PR300002 Renilla luciferase assay kit, 150 assays 1 week
PR300004 5x Passive lysis buffer 1 week
PR300005 5x Firefly luciferase lysis buffer 1 week
PR300006 Firefly luciferase assay kit, 1000 assays 1 week
PR300007 Renilla luciferase assay kit, 1000 assays 1 week
PR300008 Firefly & Renilla Luciferase Single Tube Assay Kit, 150 assays 1 week
PR300009 Firefly & Renilla Luciferase Single Tube Assay Kit, 1000 assays 1 week
PR300010 1x Passive lysis buffer 2.0 1 week