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Tet-On Inducible System

Precise, Inducible gene expression — delivered ready to use

Modified Tet-On 3G system combines high induction efficiency with minimal background expression. Order lentiviral particles or a custom stable cell line — no vector cloning required.

With validated high affinity antibodies coupled to magnetic beads (>90% high iron oxide beads), OriGene offers reliable and high specific capture of EV subpopulations from various biofluids, cell culture media.

>90%
Capture Efficiency
Low
Protein Carryover
Enriched
EV Markers
Broad
Sample Compatibility
Hero Image
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What OriGene does not sell: We do not sell inducible vectors or DNA constructs directly.

What OriGene sell: Our offerings include Tet-On Inducible Lentiviral Particles for transient or stable transduction and Custom Stable Cell Lines with validated inducible expression. 

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OriGene provides ready-to-use, expression-validated Tet-On solutions so you can move directly to your experiments. Our offerings include Tet-On Inducible Lentiviral Particles for transient or stable transduction and Custom Stable Cell Lines with validated inducible expression. Every product is functionally validated before shipment, so you can start your research from day one.

To ensure quality, consistency, and regulatory compliance, we focus exclusively on delivering validated, ready-to-use research products. For this reason, inducible vectors and DNA constructs are not available as standalone products.

What we offer

Two formats, one proven system

Choose the format that fits your workflow. Both options leverage OriGene's All-in-One Tet-On 3G system — the transactivator and TRE promoter in a single lentiviral backbone.

Ready-to-transduce

Tet-On Inducible Lentiviral Particles

High-titer lentiviral particles carrying your gene of interest under Tet-On 3G control. Transduce your cells, add doxycycline, and induce expression on your schedule.

Request lentiviral particles →
Turnkey model

Custom Tet-On Stable Cell Lines

We transduce, select, and validate a stable cell line expressing your gene of interest under inducible control. Receive a cryopreserved, characterized model — ready for your assay.

  • ●Puromycin-selected, monoclonal or polyclonal options
  • ●Induction validated by Western blot or qPCR before delivery
  • ●Compatible with a wide range of mammalian cell backgrounds
  • ●Custom GOI cloning included as part of the service
Discuss your cell line project →

System overview

How the Tet-On 3G system works

A tight, single-vector design eliminates the need for co-transduction and reduces leaky expression — the most common frustration with older inducible systems.

  • 1

    Single-vector all-in-one design

    Both the Tet-On 3G transactivator and the TRE3G (PTIGHT) promoter are encoded in one lentiviral backbone. No second plasmid or co-transduction needed.

  • 2

    Transduction and integration

    Lentiviral particles stably integrate into the host genome. The Tet-On 3G transactivator is expressed constitutively at low, non-toxic levels.

  • 3

    Doxycycline triggers expression

    Adding doxycycline (a tetracycline analog) causes the Tet-On 3G protein to bind the TRE3G promoter, driving high-level transcription of your GOI. Significant induction is observed at ≥1 µg/mL Dox.

  • 4

    Titratable, reversible control

    Expression scales with doxycycline concentration and reverses when Dox is removed — enabling dose-response studies and temporal control experiments.

System schematic

Tet-On 3G transactivator (constitutive)
↓
+ Doxycycline (≥1 µg/mL activates binding)
↓
TRE3G promoter (PTIGHT) — minimal basal activity
↓
✓ High-level GOI expression

The modified Tet-On 3G transactivator carries mutations that increase doxycycline sensitivity — particularly useful in in vivo tissues (e.g., brain) where high Dox concentrations are difficult to achieve.

Validation data

Performance backed by experiment

Our system is characterized under consistent conditions. Representative data from internal validation studies are summarized below.

Inducible Vector image
Fig 1 : FP expression in HEK293T cells transfected with plasmid DNA (1ug DNA/well of a 6-well plate) and treated with varied doxycycline (Dox) concentration for 2 days (original magnification, 10x)
Dose response of Tet-On system Graph
Fig 2 Dose response of Tet-On system with Dox as measured by the influence intensity of GFP
Effects of doxycycline on expression of TurboGFP protein by Western blotting analysis Graph
Fig 3.Effects of doxycycline on expression of TurboGFP protein by Western blotting analysis.

Why OriGene's system

What makes Tet-On 3G different

Not all Tet-On systems perform the same. Our modifications address the core limitations that frustrate researchers most.

Minimal background expression

The PTIGHT promoter combines the TRE sequence with a minimal CMV promoter, dramatically reducing leaky expression compared to conventional TRE promoters.

One vector, no co-transduction

The Tet-On 3G transactivator and TRE3G promoter are co-encoded in a single backbone, eliminating the complexity of dual-vector systems.

High Dox sensitivity

Mutations in the transactivator increase doxycycline sensitivity, enabling induction in tissues where high Dox concentrations are difficult to achieve — including the brain.

Overexpression and knockdown

The same Tet-On backbone supports both inducible GOI overexpression and inducible shRNA knockdown — giving you flexibility across experimental paradigms.

Validated before delivery

Functional titer and induction verification are included with every lot. You receive data, not just product.

No cloning required

OriGene handles vector construction and lentiviral packaging. You provide the target gene, we deliver a ready-to-use lenti particles or stable cell line.

Technical reference

Vector specifications

The vectors underlying our products are listed below for reference. These are not available for direct purchase — but understanding their architecture informs which product format to request.

Vector Mammalian selection C-tag Promoter Application
pLenti-Tet-On-GFP None tGFP PGK Reporter / induction QC
pLenti-Tet-On-Myc-DDK-Puro Puromycin Myc-DDK PGK Inducible overexpression
pLenti-Tet-On-shRNA-Puro Puromycin None PGK Inducible knockdown
pEntry-rtTA Neomycin Myc-DDK CMV Traditional (transient)
pCMV6-AC-GFP-rtTA Neomycin tGFP CMV Traditional reporter
pCMV6-AC-tGFP-rtTA-Hyg Hygromycin tGFP CMV Traditional reporter + Hyg
pCMV6-AC-Myc-DDK-rtTA-Hyg Hygromycin Myc-DDK CMV Traditional overexpression + Hyg

Lentiviral Inducible Vectors

Vector Mammalian selection C-tag Promoter Application
pLenti-Tet-On-GFP None tGFP PGK Reporter / induction QC
pLenti-Tet-On-Myc-DDK-Puro Puromycin Myc-DDK PGK Inducible overexpression
pLenti-Tet-On-shRNA-Puro Puromycin None PGK Inducible knockdown

Ready to discuss your project?

Tell us your gene of interest, target cell type, and application — we'll recommend the right format and turnaround timeline.

Vector Resources