beta Actin (beta-Actin) Mouse Monoclonal Antibody [Clone ID: OTI1]

CAT#: TA811000

beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control

Product images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-beta-actin monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:2000).

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  Western blot analysis of extracts (15ug) from 3 different tissues by using anti-beta-actin monoclonal antibody (1:2000).

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  Western blot analysis of different amount of Hela cell lysate by using anti-beta-actin monoclonal antibody (1:2000).

Specifications

Product Data

• Clone Name: OTI1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivity: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to the N terminal of human beta-actin conjugated to KLH.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.6 kDa
• Gene Name: actin, beta
• Database Link:
  NP_001092
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 403580 DogEntrez Gene 574285 MonkeyEntrez Gene 60 Human
  UniProt ID P60709
• Synonyms: BRWS1; PS1TP5BP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis