Lentiviral system for efficient DNA delivery (Lenti shRNA & LentiORF)

What is Lentivirus and why use lentivirus to deliver DNA?

Lentiviral vectors as gene delivery tool are modified from HIV-1, with many of the viral genes removed. The lentiviral vector only contain the LTRs and the packaging signal, Ψ. Lentiviral packaging genes are provided on separate plasmids, so the pseudo lentiviral particles are replication deficient.

1. Broad cell spectrum: Lentivirus infect many cells, dividing & non-dividing, easy-to-transfect & hard-to-transfect cells.
2. High transduction efficiency.
3. Convenience: Minimal need for optimization.
4. Safety: 3^rd generation system with improved biosafety.
5. Legally licensed vendor for lenti products.

Lentiviral System Validation Data

Lenti-ORF

	Plasmid
	Lentiviral Particles

Lenti shRNA

	Plasmid
	Lentiviral Particles

CRISPR

	Plasmid
	Lentiviral Particles

Lentivirus Services

	High Titer
	Flexible Volume

What is special about our lentiviral particles?

• Pre-titered, ready-to-use
• Titer guaranteed, >10^7 TU/mL
• Provided in the proprietary Lenti Stabilizer Solution with >1 year infectivity

To learn more about how to make lentivirus, please visit the lentivirus production page. The page has all you need to know to make high quality lentiviral particles, from lentivirus packaging, virus tritration, virus concentration to particle stabilization. Click to view details.

Lenti Accessories

Packaging Kit

LentiTran

Control Particles

Concentrator

Lenti Vectors

Lenti Titer Kit

Lenti Stabilizer

Lentiviral System Resources

• Lenti protocol
• Recommended lentivirus MOI for common cell lines
• Does lentivirus work with neurons?
• Frequently asked questions about lenti
• How to make lentivirus?
• Webinar: How to make lentivirus
• Lenti webinar
• Lenti biosafety

Hear what the researchers are saying about our Lenti products

"I have used shLenti for knockdown (TXN1 and TXNRD1) and they have worked well."

- A scientist from University of Texas at Austin

"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals."

- A scientist from University of Arkansas

"Lenti Vector with GFP and selection marker makes easy to generate single cell clones."

- A scientist from NIBMG

">95% of the time we achieve expression which is better than my experience with other vendors."

- A scientist from Boehringer-Ingelheim Pharmaceuticals Inc.

"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."

- A scientist from Moffitt Cancer Center

"I choose OriGene's lentivirus to induce effectively long-term and deficiency of PTP1B in a primary cell line then transplanted into mice. With OriGene Lenti we achieved a downregulation of PTP1B sustained for more than 5 days, which allowed us to assess the effect of PTP1B absence in early stages of cells engraftment!"

- A scientist from IDIBAPS

"I have used lenti-ORF plasmids for the generation of overexpression stable cells. They work well. The cell selection marker in the vector made it easier to generate stable cells."

- A scientist from Medical University of South Carolina

"The ready-to-use lentivirus particles worked well in our hard-to-transduce cell lines."

- A scientist from University Hospital of Tuebingen, Children's Hospital

"I purchased a lenti-ORF from OriGene. I have good transfection and transduction efficiency with OriGene's lenti products. Overall, I am extremely satisfied."

- A scientist from Sidney Kimmel Cancer Center / Johns Hopkins University

"I used both the lentivirus and the lenti plasmids. Both of them worked very well. And the customer service is good too."

- A scientist from St. Jude Children's Research Hospital

"We Purchased multiple lenti vectors from OriGene and they all worked great."

- A scientist from MD Anderson Cancer Center

"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."

- A scientist from The University of Queensland