OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Custom Protein Services

From Gene Cloning to Protein Purification

End-to-end recombinant protein expression, purification & modification — built around your target.

2,000+
Successful projects
500+
Satisfied customers
25+
Years of experience

We make the proteins others can't

Some proteins won't behave — large multi-domain constructs, site specific biotinylation placement, low endotoxin requirements. Those are the ones we make.

From cDNA cloning and codon optimization through expression and purification, we match the right system to your target — and hand it back with the QC data to prove it. For 25+ years, pharma, academic labs, and assay developers have relied on us for exactly that.

Watch · 90 seconds
Custom Proteins: cDNA clones to purified protein

Why teams choose OriGene

  • 12,000+ custom targets developed to date
  • 20,000+ recombinant proteins in catalog to build from
  • Transparent, milestone-based pricing — pay only for completed milestones
  • PhD-level project manager on every project
  • Developed & manufactured in the USA

Real data from recent custom projects

Custom Modification

Tag-Free Production

Tag-Free Production

Tagged (T) and tag-free (TF) GALT human recombinant protein, produced side by side with clean removal of the affinity tag.
Low-Endotoxin Production

Low-Endotoxin Production

KHDC1L human recombinant protein delivered at <0.1 EU/µg final protein, verified by Limulus (LAL) colorimetric assay.
Size Exclusion Chromatography

Size Exclusion Chromatography

SEC-FPLC used to isolate a specific dimeric state of STAT1 protein — pure-dimer fraction resolved from other species.
Expression Systems

HEK293T

HEK293T

ESR1 purified from HEK293T cells — native folding and human-like PTMs for functional studies.
Baculovirus-Insect · Sf9

Baculovirus-Insect · Sf9

IDH1 purified from Sf9 cells — high-yield eukaryotic expression for complex targets.
E. coli

E. coli

C-Myc purified from E. coli — high-yield bacterial expression for structural proteins and enzymes.
Site-Specific Biotinylation

Defined, single-site biotinylation

For applications where orientation matters — SPR/BLI kinetics, streptavidin capture, and multimer assembly — we produce protein with enzymatic AviTag (BirA) biotinylation rather than random chemical labeling.

  • One biotin at a defined position (1:1 stoichiometry)
  • Consistent, controlled orientation on streptavidin surfaces
  • Preserves the active site and binding epitope
  • Available tag-free elsewhere on the construct
Site-specific biotinylation
SDS-PAGE and Western verified site-specific biotinylated PKP2 human recombinant proteinMono-biotinylated via Avi-tagBiotin-to-protein ratio of 1:1 as determined by streptavidin pull-down assay

What a custom project can include

Expression HEK293 · Sf9 · E. coli
Tags His · GST · Fc · Myc-DDK — or tag-free
Modifications Site-specific biotinylation · low-endotoxin · SEC
Scale 100 µg – 100+ mg
QC SDS-PAGE · SEC-FPLC · LAL endotoxin
Origin US-made — Rockville, MD

A milestone-based project, start to finish

Transparent scope, PhD guidance, and pay-for-progress billing — you're only fully invoiced when your deliverables ship.

1

Fill the request form

Share your target, tags, purity, endotoxin and amount. Sequence or UniProt/NCBI ID is enough to begin.

2

Discuss scope & goals

A PhD protein scientist aligns on system, milestones, timeline and success criteria before work starts.

3

Track project progress

Receive updates and data at each milestone — expression check, purification, and QC — with go/no-go decisions.

4

Delivered & invoiced

You're only fully invoiced once the complete set of deliverables is shipped — protein plus its QC package.

Trusted by proteomics teams

OriGene has been an important partner for Alamar Biosciences, consistently providing top-tier catalog and custom recombinant antibodies and proteins. Their dependability and scientific knowledge have enabled us to offer tools that assist proteomics researchers at every step, from initial discovery to cutting-edge research.

— A scientist from Alamar Biosciences
We are very happy with the HSD17B13 protein from OriGene and found great quality for our internal quality control. We've been very happy with your customer service as well, and appreciated when there was a labeling mishap on one of the proteins — OriGene promptly provided a new corrected protein sample.

— A scientist from a pharmaceutical company

Looking for off-the-shelf proteins? Browse ready-to-ship recombinant proteins.

Ready to start your custom protein project?

Our technical team can help you select the right expression system, scope a milestone-based workflow, or arrange a bulk or custom order. Fill out the request form and we'll follow up with a milestone-based quote.

Recombinant protein resources

Match your target to the right expression system
CriteriaMammalian (HEK293)Baculovirus-Insect (Sf9)E. coli
Best forSecreted & transmembrane proteins, antibodies, PTM-dependent activityComplex proteins needing eukaryotic folding & moderate PTMs; kinases, viral proteinsAntigens, enzymes, cytokines & structural proteins
Cell typeHEK293Sf9 / Sf21E. coli
PTM · foldingSuperiorGoodLimited
GlycosylationHuman-like, complexSimplifiedNone
PhosphorylationYesYesNo
Relative yieldMedium–HighMedium–HighHigh
Relative cost$$$$$
ExampleESR1IDH1C-Myc

Not sure which system fits your target? Our scientists will recommend one based on your sequence and application.

Custom protein FAQs

 
Recombinant Protein Resources