Protocols

Antibodies and Proteins

Antibody Protocols:

• Western Blot Protocol
• IF Protocol
• FACS Protocol
• Immunoprecipitation (IP) Protocol
• IP protocol with magnetic beads
• DDK-tagged Protein Purification Protocol

ELISA and Luminex Development Guide:

• ELISA and Luminex Development Guide for Antibody Pairs
• Human MAGEA3 ELISA Kit protocol
• Human GFAP ELISA Kit protocol

IHC Center

• IHC Protocols
• Double staining protocol

Molecular Biology

Cloning:

• Transformation Protocol
• Subcloning Protocol
• Transfection Protocol
• Primer Design and PCR Amplification of ORFs Protocol
• Entry Vector Cloning Protocol
• Plasmid Reconstitution Protocol
• Primer Resuspension & Sequencing Protocol

Lentivirus:

• Lentiviral Packaging Kit Protocol
• Lentiviral Transduction Protocol

AAV:

• AAV Infection Protocol

RNAi:

• shRNA Transient Transfection Protocol
• Stable Cell Lines (Without Retroviral Infection) Protocol