Phosphorylation is a fundamental post-translational modification regulating essential cellular processes, including signal transduction, cell cycle progression, apoptosis, and differentiation. Aberrant phosphorylation is implicated in cancer, neurodegenerative diseases, and immune disorders, making precise detection of phosphorylation events critical for biomedical research.
OriGene offers more than 900 high-quality antibodies for the detection of phosphoproteins by IHC, WB.
Browse our extensive catalog of phospho-specific monoclonal and polyclonal antibodies across key signaling pathways. Optimized for multiple immunoassay techniques, including WB, IHC, FC, and ELISA.
ELISA with coating antigen P-tau217 synthetic phosphorylated peptide and P-tau217 antibody (TA592983). Negative control: coating Full length tau protein
ELISA with coating antigen P-tau181 synthetic phosphorylated peptide and P-tau181 antibody (TA594030). Negative control: coating Full length tau protein
WB analysis of extracts of K562 cells, using Phospho-STAT5-Y694 pAb (TA382100) at 1:1000 dilution.K-562 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 1s.
IHC of paraffin-embedded Human Colon Carcinoma Tissue using Phospho-ERK1/2 (Tyr222/Tyr205) antibody (TA384723). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
IHC of paraffin-embedded Human tonsils using Phospho-Smad3 (Ser425) antibody (TA385269). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.