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Antibodies for Flow Cytometry

Flow cytometry enables rapid, multi-parameter analysis of millions of cells, providing precise insights into cell phenotype, function, and state. Whether you are profiling immune cell subsets, detecting intracellular targets, performing cell cycle analysis, or sorting live populations, high-quality antibodies are essential for accuracy and reproducibility.

Our flow cytometry antibodies are validated for specificity, brightness, and consistency. Available in a wide selection of fluorescent dye conjugates, formats, and host species, our reagents support both direct and indirect detection workflows across diverse research areas including immuno-oncology, immunology, stem cell biology, neurobiology, and cytokine signaling.

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Conjugated Flow Cytometry Antibodies

Conjugated antibodies optimized with popular dyes: ABflo 450, ABflo 488, Alexa Fluor 488, DyLight 488, Alexa Fluor 546, DyLight 550, ABflow 594, DyLight 633, ABflo 647, Alexa Fluor 647, ABflo 700, APC, FITC, PE, and more... for flow cytometry experiments.

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Intracellular Flow Antibodies

Range of conjugated antibodies for intracellular flow cytometry single cell analysis.

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Unconjugated Flow Cytometry Antibodies

Unconjugated primary antibodies validated for use in flow cytometry experiments.

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Popular Conjugated Antibodies for Flow Cytometry

LGR5 Figure
FC analysis for LGR5 and CK14 expression using LGR5-PE (TA400001) antibody (view citation)
CD80 Figure
1x106 Raji cells were surface-stained with ABflo 594 Rabbit IgG isotype control (5 μl/Test,left) or ABflo 594 Rabbit anti-Human CD80/B7-1 mAb(TA427831,5 μl/Test,right).
CD2 Figure
1x106 K-562 cells (negative control,Left) and Jurkat cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human CD2 mAb(TA427799,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
B2M Figure
1x106 HeLa were surface stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test,left) or ABflo® 594 Rabbit anti-Human β2 Microglobulin mAb(TA427903,5 μl/Test,right).
CCR7 Figure
1x106 CHO(Transfection) cells were surface stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test,left) or ABflo® 594 Rabbit anti-Human Claudin18.2 mAb(TA427817,5 μl/Test,right)

Flow Antibodies for Common Immunocyte Markers

Flow cytometry is widely used to profile immune cell populations by detecting multiple immunological markers. This approach provides valuable insights for immunology research and plays an important role in understanding and treating autoimmune disorders, cancers, and other diseases.

Flow Antibodies by Research Applications or Functional Categories

Explore our flow antibodies for immunophenotyping by their function or research interests.

Common Fluorescent Dye Options

Our antibodies conjugated with popular fluorescent dye conjugates provide high stability, broad pH tolerance, and excellent compatibility with common flow cytometers and cell sorters.

Dye Name Spectra Max Excitation Wavelength (nm) Max Emission Wavelength (nm) Spectrally Similar Dyes
Alexa Fluor 488
490 525 FITC, BB515, Alexa Fluor 488, DyLight 488
FITC
491 516 Abflo 488, Alexa Fluor 488, BB515, DyLight 488
Abflo 488
491 516 FITC, BB515, Alexa Fluor 488, DyLight 488
Alexa Fluor 488
490 525 FITC, BB515, Alexa Fluor 488, DyLight 488
DyLight 488
493 518 Alexa Fluor 488, Abflow 488, FITC
PE
565 574  
Abflo 594
588 604 Alexa Fluor 594, ECD
DyLight 633
638 658 Alexa Fluor 633
Abflo 647
648 664 APC, Alexa Fluor 647, eFluor660
APC
650 660 Abflo 647, Alexa Fluor 647, eFluor660
Alexa Fluor 647
650 671 APC, Alexa Fluor 647, eFluor660
Abflo 700
690 713 Alexa Fluor 700, APC-Alexa Fluor 700

OriGene offers several custom conjugation options to meet your individual project needs. Click here to inquire

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