3’ UTR Clones
Assay-ready Luc-UTR constructs for more than 20,000 human genes
The 3' UTR plasmids provide a convenient solution for quantitative assessment of the inhibitory effect between miRNAs and their potential gene targets. OriGene’s 3' UTR clones were designed by cloning the 3' UTR sequence of a gene of interest, downstream of the firefly luciferase gene. The chimeric transcript level is then regulated by its interaction with miRNA(s), which results in varied luciferase activity quantifiable by a colorimetric assay.
» Search Your 3' UTR Clone (browse all)
Interaction between miRNA and UTR Reduced luciferase expression |
No interaction between miRNA and UTR No effect on luciferase expression |
Features
- Genome wide coverage (>20,000 human gene)
- Firefly luciferase as the easy-to-assay reporter
- RFP as reporter for transfection monitoring
- High sensitivity from IRES-driven translation of the expression cassette
Customer Reviews
Recent Citations using our 3' UTR Clones
1. MiR‐503 pleiotropically regulates epithelial‐mesenchymal transition and targets PTK7 to control lung cancer metastasis Published in Cancer Medicine, 2023 May, using SC210336 |
PubMed: 37212485 |
2. SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum Published in Cell reports, 2023 Mar, using SC214181 |
PubMed: 36848233 |
3. Oncosuppressive miRNAs loaded in lipid nanoparticles potentiate targeted therapies in BRAF-mutant melanoma by inhibiting core escape pathways of resistance Published in Oncogene, 2022 Nov, using SC209527 |
PubMed: 36418472 |