3’ UTR Clones

Assay-ready Luc-UTR constructs for more than 20,000 human genes

The 3' UTR plasmids provide a convenient solution for quantitative assessment of the inhibitory effect between miRNAs and their potential gene targets. OriGene’s 3' UTR clones were designed by cloning the 3' UTR sequence of a gene of interest, downstream of the firefly luciferase gene. The chimeric transcript level is then regulated by its interaction with miRNA(s), which results in varied luciferase activity quantifiable by a colorimetric assay.

UTR Clone
Interaction between miRNA and UTR
Reduced luciferase expression
No interaction between miRNA and UTR
No effect on luciferase expression

Features

  • Genome wide coverage (>20,000 human gene)
  • Firefly luciferase as the easy-to-assay reporter
  • RFP as reporter for transfection monitoring
  • High sensitivity from IRES-driven translation of the expression cassette