3’ UTR Clones
Assay-ready Luc-UTR constructs for more than 20,000 human genes
The 3' UTR plasmids provide a convenient solution for quantitative assessment of the inhibitory effect between miRNAs and their potential gene targets. OriGene’s 3' UTR clones were designed by cloning the 3' UTR sequence of a gene of interest, downstream of the firefly luciferase gene. The chimeric transcript level is then regulated by its interaction with miRNA(s), which results in varied luciferase activity quantifiable by a colorimetric assay.

Interaction between miRNA and UTR
Reduced luciferase expression
No interaction between miRNA and UTR
No effect on luciferase expression
Features
- Genome wide coverage (>20,000 human gene)
- Firefly luciferase as the easy-to-assay reporter
- RFP as reporter for transfection monitoring
- High sensitivity from IRES-driven translation of the expression cassette
Recent Citations using our 3' UTR Clones
1 MiR‐503 pleiotropically regulates epithelial‐mesenchymal transition and targets PTK7 to control lung cancer metastasis Published in Cancer Medicine , using |
PubMed: 37212485 |
2 SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum Published in Cell reports , using |
PubMed: 36848233 |
3 Oncosuppressive miRNAs loaded in lipid nanoparticles potentiate targeted therapies in BRAF-mutant melanoma by inhibiting core escape pathways of resistance Published in Oncogene , using |
PubMed: 36418472 |