CRISPR/Cas9 Genome Knockout Kits

Features

  • Complete kit for gene knockout via CRISPR (targeted sites around the 5’ end of the ORF)
  • 2 guide RNA vectors in pCas-Guide to ensure an efficient cleavage
  • Donor vector with predesigned homologous arms
  • Knockin GFP-Puro for selection
  • pCas-Guide-scramble is also provided as a negative control

Applications

  • Knock-in GFP reporter for promoter study
  • Knock-out genes at chromosomal level

Find Your Knockout Kits

CRISPR Manual      Knockout Kit Validation

Scheme of Genome-editing knockout kit using CRISPR

Diagram-Gene Editing RecombII

Cell Lines Tested Publications SKU
A549 Int J Mol Sci 2017 KN200003
A549/DX Oncotarget 2016 KN204034
bEnd5 Cell Commun. Signal 2016 KN316845
Cal27 Oncotarget 2017 KN204922
DU145 Anal. Biochem. 2017 KN210866
GLC82 Oncotarget 2017 KN214877
Hela J. Cell Biol. 2016 KN206187
HEp-2 Proc. Natl. Acad. Sci. U.S.A. May 2016 KN200700
HT29/DX Oncotarget 2016 KN204034
MCF10A Oncotarget 2016 KN204034
MCF10A Anal. Biochem. 2017 KN210866
MDA-MB-231 Anal. Biochem. 2017 KN210866
MG63 DNA Cell Biol 2017 KN202627
PANC-1 Am J Physiol Gastrointest Liver Physiol. 2016 KN204066
SaOs-2 Sci Rep May 2016 KN206029
SH-SY5Y J. Biol. Chem. 2016 KN216220
T84 Oncotarget 2017 KN209079
U2OS Anal. Biochem. 2017 KN210866
U2OS/DX Oncotarget 2016 KN204034
Gene Expression Resources