CRISPR/Cas9 Genome Knockout Kits

CRISPR/Cas9 is a simple and efficient genome editing tool. Although gene knockout cell lines can be generated by gRNAs without donor vector, the screening process can be very tedious. OriGene offers genome-wide CRISPR gene knockout / knockin kits containing 2 gRNA vectors and donor DNA. The selection marker in the donor cassette greatly facilitates the screening process.

Two types of CRISPR knockout kits are offered, differing in repair mechanism that is used to achieve gene knockout, homology based or non-homology based. HDR-mediated knockout kit is our first generation gene knockout kits, gene knockout is achieved via homology directed repair (HDR). KN2.0 gene knockout kit is an improved version, gene knockout is achieved via non-homology based repair. KN2.0 CRISPR knockout kit is more efficient and works in both dividing and non-dividing cells.

  1. Non-homology mediated CRISPR gene knockout kits (KN2.0)    NEW
  2. HDR mediated CRISPR gene knockout kits

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Comparison of CRISPR gene knockout kits

HDR mediated KN2.0 non-homology mediated
gRNA vectors pCas-Guide pCas-Guide
Donor Donor cassette flanking with homologous arms Donor cassette without homologous arms
Cell spectrum Dividing cells Dividing and non-dividing cells
Knockout Efficiency Medium High

HDR-mediated knockout kit validation      KN2.0 knockout kit validation

Find Your Knockout Kits


KN2.0 non-homology mediated CRISPR gene knockout kits     NEW

Gene knockout is based on non-homology-mediated repair mechanism. After gRNA targeted double stranded DNA cleavage, the linear donor DNA containing a selection cassette will be integrated at the gRNA cutting site at forward or reverse direction. Our testing data using KN2.0 kits showed that the vast majoiry gene knockout is bioallelic, one allele contains donor integration, the other allele has indels (insertion and deletion), which might change protein coding or cause premature stop.

Features

  • Ready-to-use gene knockout kit
  • 2 guide RNA vectors in pCas-Guide
  • Donor cassette, EF1a-GFP-P2A-Puro
  • Non-homology mediated gene knockout
  • Highly efficient gene knockout kit

Scheme of gene knockout of KN2.0

KN2.0 CRISPR Gene Knockout Diagram

HDR mediated CRISPR knockout kits

Gene knockout is achieved via homology-directed repair (HDR) mechanism. Donor vector contains homologous sequences flanking the selection cassette. After gRNA targeted double stranded break, donor cassette is inserted in the genome via homologous repair immediately downstream the native promoter.

Features

  • Complete kit for gene knockout via CRISPR (targeted sites around the 5’ end of the ORF)
  • 2 guide RNA vectors in pCas-Guide to ensure an efficient cleavage
  • Donor vector with predesigned homologous arms
  • pCas-Guide-scramble is also provided as a negative control
  • 4 different donor selection cassette available for each gene


    HDR CRISPR gene knockout donor

Applications

  • Knock-in a reporter for promoter study
  • Knock-out genes at chromosomal level

Scheme of gene knockout of HDR mediated kit

Diagram-Gene Editing RecombII

Other CRISPR Products


CRISPR Gene Knockout Kit Citations and Cell Lines Tested

Cell Lines Tested Publications SKU
A549 Int J Mol Sci 2017 KN200003
A549/DX Oncotarget 2016 KN204034
bEnd5 Cell Commun. Signal 2016 KN316845
Cal27 Oncotarget 2017 KN204922
DU145 Anal. Biochem. 2017 KN210866
GLC82 Oncotarget 2017 KN214877
Hela J. Cell Biol. 2016 KN206187
HEp-2 Proc. Natl. Acad. Sci. U.S.A. May 2016 KN200700
HT29/DX Oncotarget 2016 KN204034
MCF10A Oncotarget 2016 KN204034
MCF10A Anal. Biochem. 2017 KN210866
MDA-MB-231 Anal. Biochem. 2017 KN210866
MG63 DNA Cell Biol 2017 KN202627
PANC-1 Am J Physiol Gastrointest Liver Physiol. 2016 KN204066
SaOs-2 Sci Rep May 2016 KN206029
SH-SY5Y J. Biol. Chem. 2016 KN216220
T84 Oncotarget 2017 KN209079
U2OS Anal. Biochem. 2017 KN210866
U2OS/DX Oncotarget 2016 KN204034