IntactProtein™ Cell/Tissue Lysis Buffer
Efficient protein extraction is critical for reliable Western blotting and proteomic analyses. The IntactProtein Cell/Tissue Lysis Buffer is designed to provide ultimate solution for extracting large proteins from mammalian cells and tissues (including adipose tissue) while preserving post-translational modifications (PTMs). Unlike conventional lysis buffers that require additional inhibitors and optimization, this ready-to-use solution ensures maximum protein integrity with minimal effort.
Key Features & Benefits
- High-Yield Extraction: Efficiently recovers proteins of all sizes, including large molecular weight proteins often lost with RIPA buffer.
- Preserves PTMs: Maintains phosphorylation, acetylation, and glycosylation states for accurate downstream analysis.
- All-in-one formula: No protease/other enzyme inhibitors or sonication required.
- Gentle Yet Effective Lysis: Suitable for cytoplasmic, membrane, nuclear, and organelle protein extraction without sonication.
- Broad Applications: Extract protein from mammalian, cell, and tissues (including adipose tissue). Ideal for Western blot and other proteomics applications.
Case Studies
The IntactProtein lysis buffer demonstrates superior performance in extracting large protein comparison to RIPA buffer. 50 µg of HeLa cell lysed using IntactProtein or RIPA buffer.
The IntactProtein lysis buffer is effective in extracting proteins from cell lysates and tissue samples.
The IntactProtein lysis buffer is effective in preserving protein post-translation modifications. (A-B). Serum starved and Insulin stimulated HeLa cells were lysed using IntactProtein. 50 µg of total lysate were immunoblotted with anti-phospho mTOR (Ser2448) and anti-mTOR antibodies. (C) HeLa cell lysate immunoblotted using anti-O-GlcNAc (glycosylation) antibody and compared to RIPA and IntactProtein lysis.
Comparison: IntactProtein vs. Traditional RIPA Buffer
| Features | IntactProtein Lysis Buffer | RIPA Buffer |
|---|---|---|
| Protein Yield | High (all sizes) | Medium |
| PTM Preservation | Yes | Limited |
| Pre-Added Inhibitors | Yes | No (must be added manually) |
| Large Protein Extraction | Optimized | Poor |
| Workflow Simplicity | Ready-to-use | Require multiple reagents |
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