High Performance Transfection with TurboFectin 8.0

The ultimate in high efficiency and low toxicity!

TurboFectin 8.0 is a new generation of transfection reagent optimized for nucleic acid delivery into eukaryotic cells. Its proprietary formulation of lipid/histone blend is supplied in 80% alcohol. TurboFectin 8.0 is the recommended transfection reagent for delivery of TrueClone/TrueORF Clone (for over-expression) and HuSH-29 constructs (shRNA for expression knock-down) in many different cell types.

  • Wide spectrum: validated on > 100 cell lines and primary cell types (Click for list).
  • Recommended for shRNA-induced silencing (click for data).
  • Preferred reagent for reverse transfection assays.
  • Simplified use: Works well in media containing antibiotic and antimycotic agents. Suitable for serum-containing media; no requirement for media change.
  • Affordability: One mL can be used for 300-500 transfections of 6-well plate cultures, < $0.90 per transfection.
Catalog No. Description
TF81001 TurboFectin 8.0 (1 vial @ 1ml each)
TF81005 TurboFectin 8.0 (5 vials @ 1ml each)


Cell lines and primary cells have been efficiently transfected by TurboFectin

3T3-L1HEC-1AMDA-MB-435primary mouse embryo niccells
A1847HEC-1BMDCKprimary mouse fibroblasts
A549HEK293MELprimary mouse hepatocytes
AR42ce4HeLamelanocyteprimary mouse myotubes
ARP1Hepa1-6Mv1Luprimary mouse thymocytes
BHK-21Hepa1cLc7myometrialprimary rat hepatocytes
BNL.CL2HepG2Neuro-2aprimary rat osteoblasts
BRL-3AHLF-aneuroepithelialrat smooth muscle cells
chicken embryo fibroblasthuman bladder carcinomaovarian cancerRBL-2H3
corneal endotheliumK562OVCAR4SK-N-MC
DI-TNC1MA-10primary human astrocytesVero
DU145MC3T3-E1primary human chondrocytesWB rat liver epithelial cells
ECV304MCF-7primary human keratinocytesWiDr
H1299MDA-MB-231primary human melanoma cellsWRL-68
HCT-116 primary human skin fibroblasts 

TurboFectin has been cited:

Targeting ABL-IRE1α Signaling Spares ER-Stressed Pancreatic β Cells to Reverse Autoimmune DiabetesCell Metab. 2017

YBX1 gene silencing inhibits migratory and invasive potential via CORO1C in breast cancer in vitroBMC Cancer 2017

A Novel DNA Vaccine Platform Enhances Neo-antigen-like T Cell Responses against WT1 to Break Tolerance and Induce Anti-tumor ImmunityMol. Ther 2017

Targeting CD133 improves chemotherapeutic efficacy of recurrent pediatric pilocytic astrocytoma following prolonged chemotherapyMol. Cancer 2017

Different non-synonymous polymorphisms modulate the interaction of the WRN protein to its protein partners and its enzymatic activitiesOncotarget 2016

N-Cadherin, a novel and rapidly remodelling site involved in vasoregulation of small cerebral arteriesJ. Physiol. (Lond.) 2016

GSDMB induces an asthma phenotype characterized by increased airway responsiveness and remodeling without lung inflammationProc. Natl. Acad. Sci. U.S.A. 2016

Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafishPhysiological Reports 2016

Efficient generation and reversion of chromosomal translocations using CRISPR/Cas technologyBMC Genomics 2016

Forkhead box A2 regulated biliary heterogeneity and senescence during cholestatic liver injuryHepatology 2016

Parallel reverse genetic screening in mutant human cells using transcriptomicsMol. Syst. Biol. 2016

Fulvestrant inhibits growth of triple negative breast cancer and synergizes with tamoxifen in ERα positive breast cancer by up-regulation of ERβOncotarget 2016

DNA polymerase ? functions in the generation of tandem mutations during somatic hypermutation of antibody genesJ. Exp. Med. 2016

Suppression of MTHFD2 in MCF7 breast cancer cells increases glycolysis, dependency on exogenous glycine and sensitivity to folate depletionJ. Proteome Res. Jun 2016

Endometrial MFG-E8 regulates TGF-ß induced EMT promoting trophoblast invasion and implantationReproduction Jun 2016

miRNA-106a directly targeting RARB associates with the expression of Na(+)/I(-) symporter in thyroid cancer by regulating MAPK signaling pathwayJ. Exp. Clin. Cancer Res. Jun 2016

The DEK oncogene activates VEGF expression and promotes tumor angiogenesis and growth in HIF-1a-dependent and -independent mannersOncotarget 2016

A phosphomimetic-based mechanism of dengue virus to antagonize innate immunityNat. Immunol. Mar 2016

The enzyme L-isoaspartyl (D-aspartyl) methyltransferase is required for VEGF-dependent endothelial cell migration and tubulogenesisMol. Cell. Biochem. Jan 2016

A generic strategy for CRISPR-Cas9-mediated gene taggingNat Commun Dec 2015

Differential Mechanisms of Tenofovir and Tenofovir Disoproxil Fumarate Cellular Transport and Implications for Topical Pre-exposure ProphylaxisAntimicrob. Agents Chemother. Dec 2015

Early Changes in Cytochrome P450s and Their Associated Arachidonic Acid Metabolites Play a Crucial Role in the Initiation of Cardiac Hypertrophy Induced by IsoproterenolDrug Metab. Dispos. Aug 2015

Inhibition of dipeptidyl peptidase 8/9 impairs preadipocyte differentiationSci Rep Aug 2015

Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) productionRetrovirology Aug 2015

Slit2 Promotes Angiogenic Activity Via the Robo1-VEGFR2-ERK1/2 Pathway in Both In Vivo and In Vitro StudiesInvest. Ophthalmol. Vis. Sci. Aug 2015

Early Changes in Cytochrome P450s and Their Associated Arachidonic Acid Metabolites Play a Crucial Role in the Initiation of Cardiac Hypertrophy Induced by Isoproterenol.Drug Metab. Dispos. 2015

9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediateNat Commun Jul 2015

Reorganization of metastamiRs in the evolution of metastatic aggressive neuroblastoma cellsBMC Genomics Jul 2015

Novel synthetic plasmid and doggybone™ DNA vaccines induce neutralizing antibodies and provide protection from lethal influenza challenge in miceHum Vaccin Immunother Jun 2015

The role of the NOD-like receptor, Nlrx1 in neuroprotection and cell deathThesis Jun 2015

Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored ProteinsCell Rep Jun 2015

CD11b(+) myeloid cells support hepatic metastasis through downregulation of Angiopoietin-like 7 in cancer cellsHepatology Apr 2015

Novel adjuvants from seaweed impede autophagy signaling in therapy-resistant residual pancreatic cancerJ. Biomed. Sci. Apr 2015

CD133 and DNA-PK regulate MDR1 via the PI3K- or Akt-NF-?B pathway in multidrug-resistant glioblastoma cells in vitroOncogene Mar 2015

SIRT5 regulation of ammonia-induced autophagy and mitophagyAutophagy Feb 2015

MicroRNA-375 plays a dual role in prostate carcinogenesisclinical epigenetics journal Jan 2015

Novel and enhanced anti-melanoma DNA vaccine targeting the tyrosinase protein inhibits myeloid-derived suppressor cells and tumor growth in a syngeneic prophylactic and therapeutic murine modelCancer Gene Ther. Nov 2014

Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic modelSci Rep Oct 2014

Identification of the Major Prostaglandin Glycerol Ester Hydrolase in Human Cancer CellsJ. Biol. Chem. Oct 2014

EphrinB1 expression is dysregulated and promotes oncogenic signaling in medulloblastomaJ. Neurooncol. Sep 2014

Using the atomic force microscope as a nanomechanical partner to support evanescent field imagingThe European Physical Journal Special Topics Sep 2014

Restored immunosuppressive effect of mesenchymal stem cells on B cells after OAZ downregulation in patients with systemic lupus erythematosusArthritis & rheumatology Sep 2014

Saturation editing of genomic regions by multiplex homology-directed repairNature Aug 2014

Lentivector integration sites in ependymal cells from a model of metachromatic leukodystrophy: non-B DNA as a new factor influencing integrationMol Ther Nucleic Acids Aug 2014

Gene expression profiling identifies FYN as an important molecule in tamoxifen resistance and a predictor of early recurrence in patients treated with endocrine therapyOncogene Jun 2014

Nonviral gene transfer to human meniscal cells. Part I: transfection analyses and cell transplantation to meniscus explantsInt Orthop Jun 2014

Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccineVaccine Mar 2014

Dopamine down-regulation of protein L-isoaspartyl methyltransferase is dependent on reactive oxygen species in SH-SY5Y cellsNeuroscience Mar 2014

Posttranslational modification of mitochondrial transcription factor A in impaired mitochondria biogenesis: Implications in diabetic retinopathy and metabolic memory phenomenonExp. Eye Res. Mar 2014

Co-Administration of Molecular Adjuvants Expressing NF-Kappa B Subunit p65/RelA or Type-1 Transactivator T-bet Enhance Antigen Specific DNA Vaccine- …Vaccines Mar 2014

Cytochrome P450 2C Epoxygenases Mediate Photochemical Stress-induced Death of PhotoreceptorsJ. Biol. Chem., Feb 2014.

The Steric Gate of DNA Polymerase ι Regulates Ribonucleotide Incorporation and Deoxyribonucleotide FidelityJ. Biol. Chem., Feb 2014.

A Cell-Based High-Throughput Screen Addressing 3'UTR-Dependent Regulation of the MYCN GeneMol. Biotechnol., Feb 2014.

The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cellsEMBO Mol Med, Jan 2014.

UBE2Q1 expression in human colorectal tumors and cell linesMol. Biol. Rep. 7045-51 Nov 2013

A Missense Mutation in CRYBB2 Leads to Progressive Congenital Membranous Cataract by Impacting the Solubility and Function of ßB2-CrystallinPLOS ONE e81290 Nov 2013

Targeting annexin A7 by a small molecule suppressed the activity of phosphatidylcholine-specific phospholipase C in vascular endothelial cells and inhibited atherosclerosis in apolipoprotein E????iceCell Death Dis. 2013 Sep 19;4:e806

Temozolomide downregulates P-glycoprotein expression in glioblastoma stem cells by interfering with the Wnt3a/glycogen synthase-3 kinase/ß-catenin pathwayNeuro Oncology, Jul 2013; 10.1093/neuonc/not104.

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) CycleJ. Biol. Chem., May 2013; 288: 12967 - 12977.

Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1Am J Physiol Cell Physiol, Apr 2013; 304: C768 - C779.

Regulation of interleukin-6 secretion by the two-pore-domain potassium channel Trek-1 in alveolar epithelial cellsAm J Physiol Lung Cell Mol Physiol, Feb 2013; 304: L276 - L286.

Ubiquitin mediates the physical and functional interaction between human DNA polymerases andNucleic Acids Res., Dec 2012; 10.1093/nar/gks1277.

Structurally similar estradiol analogs uniquely alter the regulation of intracellular signaling pathwaysJ. Mol. Endocrinol., Dec 2012; 50: 43 - 57.

Intracellular Endothelin Type B Receptor-driven Ca2+ Signal Elicits Nitric Oxide Production in Endothelial CellsJ. Biol. Chem., Nov 2012; 287: 41023 - 41031.

Secretomic Analysis Identifies Alpha-1 Antitrypsin (A1AT) as a Required Protein in Cancer Cell Migration, Invasion, and Pericellular Fibronectin Assembly for Facilitating Lung Colonization of Lung Adenocarcinoma CellsMol. Cell. Proteomics, Nov 2012; 11: 1320 - 1339.