Stable Cell Line Development Service

Generating custom cell lines is a tedious and complex process. OriGene offers a custom stable cell line development service that can be designed for knockdown, knock-in, or knockout of virtually any protein of interest. Take advantage of our expertise in gene expression and highly effective gene delivery technologies to produce high-quality cell lines. Request a free quote and we will put you in contact with our team of experts to design your cell line to meet your project requirements.

What We Offer:

  • Over Expression (ORF)
  • Knockdown (shRNA)
  • Knock-in (CRISPR)
  • Knockout (CRISPR)

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SL500077SL500075
Figure 1: Validation data of HEK293T (right, Cat # SL500075) and MDA-MB-231 (left, Cat #SL500077) cell lines overexpressing GFP.

Case Studies: Over-expression in Stable Cell Lines

A human gene (Target X) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target X and ß-actin antibody (OriGene, TA811000) demonstrates over-expression of Target X in Clones 1, and at a higher level in Clone 2 (OriGene internal data).

A human gene (Target Y) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target Y and ß-actin antibody (OriGene, TA811000) demonstrates over-expression of Target Y in the stable cell line (OriGene internal data).

Service Highlights:

  • Fast turnaround time: 2-3 months
  • Choose from OriGene's extensive ORF clone collection or provide your own expression cassette
  • Rigorous and customizable QC validation process
  • Professional, Global Technical Support

Cell Line Development Project Design

At OriGene, you have the power to customize every step of the stable cell line development process. From vector selection to final delivery, we offer a spectrum of options designed to cater to your specific needs.

Vector Selection
  • Subcloning
  • Gene Synthesis
  • Sequencing/ Expression Validation (TrueORF Gold)
Cell Line Development
  • Cell line selection
  • Viral/non-viral transfection options
Cell Line Validation
  • Western Blot
  • Mycoplasma Detection
  • Custom options available
Delivery
  • 2-3 months

GFP Controls for your Over-expression Experiments:

Name Description Catalog #
GFP Over-expression Stable Cell Line - HEK293T GFP Over-expression Stable Cell Line - HEK293T, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500075
GFP Over-expression Stable Cell Line - Hela GFP Over-expression Stable Cell Line - Hela, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500076
GFP Over-expression Stable Cell Line - MDA-MB-231 GFP Over-expression Stable Cell Line - MDA-MB-231, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500077
GFP Over-expression Stable Cell Line - HEK293 GFP Over-expression Stable Cell Line - HEK293, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500078