Stable Cell Line Development Service

Generating custom cell lines is a tedious and complex process. OriGene offers a custom stable cell line development service that can be designed for over-expression of virtually any protein of interest (constitutive and inducible options available). Take advantage of our expertise in gene expression and highly effective gene delivery technologies to produce high-quality cell lines. Request a free quote and we will put you in contact with our team of experts to design what you need.

Constitutive Over Expression:
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Inducible Over Expression:
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Service Highlights:

  • Fast turnaround time: 2-3 months
  • Choose from OriGene’s extensive ORF clone collection or provide your own expression cassette
  • Rigorous and customizable QC validation process
  • Professional, Global Technical Support

Cell Line Development Workflow

Vector Selection
  • Subcloning
  • Gene Synthesis
  • Sequencing/ Expression Validation (TrueORF Gold)
  • Inducible vs. Standard
Cell Line Development
  • Cell line diversity
  • Viral/non-viral technique
Cell Line Validation
  • Western Blot
  • Mycoplasma Detection
  • Custom
  • Constitutive: 2-3 months
  • Inducible: 3-4 months

OriGene has over 25 years of experience in developing stable cell lines

We conduct various QC processes throughout the development stage to ensure that the final product is of the highest quality. Many of our clones (TrueORF Gold) are sequence verified, expression validated, and in stock, which enables us to have a faster turnaround time when developing stable cell lines. All of our generated cell lines are tested for Mycoplasma contamination using our highly sensitive Mycoplasma Detection Kit (NP100050) as well as validated by western blot.

Stable cell linesStable cell lines
Figure 1: QC data from the generation of 4 HEK293T stable cell lines. Right: Western blot analysis of extracts from 4 different cell lines by using anti-beta-actin monoclonal antibody (TA811000S). Left: Overexpression Stable cell lines (HEK293T cells) were tested for Mycoplasma contamination using MycoTrack (NP100050).

Inducible Stable Cell Line Development

Inducible expression is a valuable tool for functional analysis of assay systems with toxic targets. OriGene's All-in-one Tet-On system is a new and improved version of the original Tet-On systems, designed to significantly stimulate expression of the downstream gene of interest (GOI). Click here to learn more about our inducible vectors.

Stable cell lines
Figure 2. Human GPR37 cDNA gene was cloned into a mammalian Tet-On inducible vector (PS100133). HEK293T cells were transfected with a PS100133-GPR37 construct (0.5 µg DNA/well of a 12-well plate). Single stable HEK293T colonies were established after 4 weeks of hygromycin drug selection at 200 µg/mL and then treated with or without doxycycline (Dox) at 5 µg/mL for 3 days. High GFP expression was observed by the treatment of Dox for 3 days.