Stable Cell Line Development Service
Generating custom cell lines is a tedious and complex process. OriGene offers a custom stable cell line development service that can be designed for knockdown, knock-in, or knockout of virtually any protein of interest. Take advantage of our expertise in gene expression and highly effective gene delivery technologies to produce high-quality cell lines. Request a free quote and we will put you in contact with our team of experts to design your cell line to meet your project requirements.
What We Offer:
- Over Expression (ORF)
- Knockdown (shRNA)
- Knock-in (CRISPR)
- Knockout (CRISPR)
Figure 1: Validation data of HEK293T (right, Cat # SL500075) and MDA-MB-231 (left, Cat #SL500077) cell lines overexpressing GFP. |
Case Studies: Over-expression in Stable Cell Lines
A human gene (Target X) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target X and ß-actin antibody (OriGene, TA811000) demonstrates over-expression of Target X in Clones 1, and at a higher level in Clone 2 (OriGene internal data).
A human gene (Target Y) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target Y and ß-actin antibody (OriGene, TA811000) demonstrates over-expression of Target Y in the stable cell line (OriGene internal data).
Service Highlights:
- Fast turnaround time: 2-3 months
- Choose from OriGene's extensive ORF clone collection or provide your own expression cassette
- Rigorous and customizable QC validation process
- Professional, Global Technical Support
Cell Line Development Project Design
At OriGene, you have the power to customize every step of the stable cell line development process. From vector selection to final delivery, we offer a spectrum of options designed to cater to your specific needs.
- Subcloning
- Gene Synthesis
- Sequencing/ Expression Validation (TrueORF Gold)
- Cell line selection
- Viral/non-viral transfection options
- Western Blot
- Mycoplasma Detection
- Custom options available
- 2-3 months
GFP Controls for your Over-expression Experiments:
Name | Description | Catalog # |
---|---|---|
GFP Over-expression Stable Cell Line - HEK293T | GFP Over-expression Stable Cell Line - HEK293T, 1 vial, 3 x 10^6 cells/vial, Cryopreserved | SL500075 |
GFP Over-expression Stable Cell Line - Hela | GFP Over-expression Stable Cell Line - Hela, 1 vial, 3 x 10^6 cells/vial, Cryopreserved | SL500076 |
GFP Over-expression Stable Cell Line - MDA-MB-231 | GFP Over-expression Stable Cell Line - MDA-MB-231, 1 vial, 3 x 10^6 cells/vial, Cryopreserved | SL500077 |
GFP Over-expression Stable Cell Line - HEK293 | GFP Over-expression Stable Cell Line - HEK293, 1 vial, 3 x 10^6 cells/vial, Cryopreserved | SL500078 |