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Stable Cell Line
Development Service

Why go through the tedious process of stable cell line development?
Pass your project to our experts and utilize OriGene's extensive expertise and proven methodologies.

awesomeicons/solid/flask Tet-on Inducible Expression awesomeicons/solid/bolt Over Expression (ORF) awesomeicons/solid/shield-alt Knockdown & Knockout
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What We Offer

Comprehensive stable cell line development services tailored to your research needs

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2-3

Months Turnaround

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35k+

ORF Clones Available

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24/7

Technical Support

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Tet-on Inducible Expression

Control gene expression with doxycycline. Ideal for functional analysis of toxic targets with our All-in-one Tet-On system.

  • Dose-dependent control
  • Reversible expression
  • Reduced toxicity
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Over Expression (ORF)

Constitutive over-expression of your gene of interest using our extensive ORF clone collection or your custom cassette.

  • 35,000+ ORF clones
  • Custom vectors
  • Multiple cell lines
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Knockdown & Knockout

Gene silencing using shRNA or CRISPR/Cas9 technology for loss-of-function studies and target validation.

  • CRISPR/Cas9
  • shRNA knockdown
  • Complete knockout

Cell Line Development
Workflow

A customizable process designed to meet your specific research requirements

Vector Selection

Choose from subcloning, gene synthesis, or TrueORF Gold. Select inducible vs. constitutive expression.

  • Subcloning options
  • Gene synthesis
  • Sequencing validation
  • Expression validation

Cell Line Development

Select your preferred cell line and transfection method for optimal results.

  • HEK293, CHO, or custom
  • Viral transfection
  • Non-viral transfection
  • Single cell cloning/li>

Validation & QC

Rigorous quality control process with customizable validation options.

  • Western Blot
  • Mycoplasma detection
  • Flow cytometry
  • Custom assays

Delivery

Receive your validated stable cell lines ready for downstream applications.

  • Constitutive: 2-3 months
  • Inducible: 3-4 months
  • Cryopreserved vials
  • Complete documentation

Vector Selection

Choose from subcloning, gene synthesis, or TrueORF Gold. Select inducible vs. constitutive expression.
  • Subcloning options
  • Gene synthesis
  • Sequencing validation
  • Expression validation

Cell Line Development

Select your preferred cell line and transfection method for optimal results.
  • HEK293, CHO, or custom
  • Viral transfection
  • Non-viral transfection
  • Single cell cloning

Validation & QC

Rigorous quality control process with customizable validation options.
  • Western Blot
  • Mycoplasma detection
  • Flow cytometry
  • Custom assays

Delivery

Receive your validated stable cell lines ready for downstream applications.
  • Constitutive: 2-3 months
  • Inducible: 3-4 months
  • Cryopreserved vials
  • Complete documentation

Research Applications

Stable cell lines are essential tools across multiple research areas and applications

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Target Validation

Validate drug targets using over-expression or knockdown cell lines for functional studies.

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Drug Discovery

Screen compound libraries against stable cell lines expressing your target of interest.

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Functional Studies

Study gene function, protein interactions, and signaling pathways in stable expression systems.

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Assay Development

Develop robust and reproducible assays with consistent gene expression levels.

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Protein Production

Produce recombinant proteins for research, structural studies, or therapeutic development.

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Toxicity Studies

Use inducible systems to study toxic targets with controlled expression timing.

Inducible Lentiviral Particles for Overexpression

Inducible
Figure 2. Dose-dependent GFP expression in HEK293T cells transduced with inducible lentiviral GFP particles and treated with varied Dox concentrations for 5 days (original magnification, 10x). High GFP expression was observed by the treatment of 10 µg/mL Dox.

GFP Controls for your Over-expression Experiment

Name Description Catalog # Cart
GFP Over-expression Stable Cell Line - HEK293T GFP Over-expression Stable Cell Line - HEK293T, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500075
GFP Over-expression Stable Cell Line - Hela GFP Over-expression Stable Cell Line - Hela, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500076
GFP Over-expression Stable Cell Line - MDA-MB-231 GFP Over-expression Stable Cell Line - MDA-MB-231, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500077
GFP Over-expression Stable Cell Line - HEK293 GFP Over-expression Stable Cell Line - HEK293, 1 vial, 3 x 10^6 cells/vial, Cryopreserved SL500078

Choose the Right Approach

Compare different technologies to find the best solution for your research needs

Technology Turnaround Expression Control Toxic Targets Expression Level Best For
Constitutive Expression
Continuous gene expression		 2–3 months ✕ ✕ High & stable Non-toxic targets requiring consistent expression
Tet-On Inducible
Dox-controlled expression		 3–4 months ✓ ✓ Dose-dependent Toxic targets or studies requiring expression control
shRNA Knockdown
Gene silencing via RNA interference		 2–3 months – ✓ 70–90% reduction Partial gene silencing studies
CRISPR Knockout
Complete gene disruption		 3–4 months ✕ – Complete KO Complete loss-of-function studies

Ready to Start Your Project?

Let our experts handle your stable cell line development. Get a customized quote for your specific project requirements.