TrueORF Gold

TrueORF Gold

TrueORF Gold is OriGene's collection of premium cDNA clones that have passed the ultimate tests: expression validation & sequence verification. Using non-validated clones can result in inaccurate or absent expression. Don't waste your time and valuable resources. Order a TrueORF Gold clone and start your experiment today!

TrueORF""

TrueORF Gold delivers quality and promptness in one tube.

Expression Validated by Western Blot

Each TrueORF Gold clone has been used to transfect human cell lines for protein expression. In fact, OriGene has produced over 12,000 over-expression lysates from TrueORF-transfected HEK293 cells and subsequently purified over 5,000 human proteins from those cells.

Sequence Verified

Each TrueORF Gold clone is fully sequence with down-loadable chromatogram files posted online. View and analyze the sequences before purchasing and you will never worry about mutations when using TrueORF Gold.

Next Day Shipment

All TrueORF Gold clones are available for next day shipment. Why spend weeks constructing a cDNA clone or have it synthesized? TrueORF Gold accelerates your research and gets you there faster, your experiment, your degree, your publication or your grant application.

Transfection Ready
  • No need for subcloning. No need for plasmid preparation. Order a TrueORF Gold clone today, and start your experiment tomorrow.
  • TrueORF Gold clones are constructed with all elements (promoter, polyadenylation signal, drug-selection marker, etc) for protein-expression in mammalian cells.
  • TrueORF Gold clones are produced with high purity and low endotoxicity.

Easily Shuttled into over 100+ Vectors

OriGene has prepared over a long list of shuttle vectors with matching cloning sites for easy transfer of the insert using PrecisionShuttle, a simple cut-and-paste procedure. Upon transfer, the highly validated inserts are automatically put into a different context for alternative expression options

  • Fluorescent-protein tagging vectors
  • Epitope-tagging vectors
  • Selection markers
  • Bacterial expression vectors

Each TrueORF Gold clone has been used to transfect human cell lines for protein expression. In fact, OriGene has produced over 12,000 over-expression lysates from TrueORF-transfected HEK293 cells and subsequently purified over 5,000 human proteins from those cells.

Each TrueORF Gold clone is fully sequence with down-loadable chromatogram files posted online. View and analyze the sequences before purchasing and you will never worry about mutations when using TrueORF Gold.

All TrueORF Gold clones are available for next day shipment. Why spend weeks constructing a cDNA clone or have it synthesized? TrueORF Gold accelerates your research and gets you there faster, your experiment, your degree, your publication or your grant application.

  • No need for subcloning. No need for plasmid preparation. Order a TrueORF Gold clone today, and start your experiment tomorrow.
  • TrueORF Gold clones are constructed with all elements (promoter, polyadenylation signal, drug-selection marker, etc) for protein-expression in mammalian cells.
  • TrueORF Gold clones are produced with high purity and low endotoxicity.

OriGene has prepared over a long list of shuttle vectors with matching cloning sites for easy transfer of the insert using PrecisionShuttle, a simple cut-and-paste procedure. Upon transfer, the highly validated inserts are automatically put into a different context for alternative expression options

  • Fluorescent-protein tagging vectors
  • Epitope-tagging vectors
  • Selection markers
  • Bacterial expression vectors

Comparison of cDNA clones from different sources

  Sequence-Verification Expression Validation Wait Time Shuttling into 100+ vectors
Yes Yes None Yes
Clones constructed in your lab Y/N Y/N Varies No
Clones obtained from other vendors Y/N No Varies No
Clones obtained by gene-synthesis Yes No Long No

Customer Reviews

"We are a loyal local customer of OriGene and we always get reliable results from using their expression plasmids."
Reviewed By J.S.
CiPA Lab
"Origene had the exact clone we needed."
Reviewed By M.B.
University of Pennsylvania
"Origene products are easy to use and customer service is super helpful! I've always got helped when I encountered a problem. Thank you."
Reviewed By T.T.
University of Michigan
"What I like about the Origene wesbsite was that the chat line usually directs me to the right person to ask questions -- who not only answers my questions, but will provide the quote, and good to follow up on the progress, etc."
Reviewed By R.A.
Case Western Reserve University
"Good quality, fast turnaround time, reasonable pricing with a vast selection"
Reviewed By J.Z.
BC Cancer Research Centre
"I really trust OriGene's cloning service!"
Reviewed By A.C.
Icahn School of Medicine in Mount Sinai
"Our lab is a frequent customer."
Reviewed By P.B.
George Washington University, School of Medicine
"The technical support was great with good response time."
Reviewed By L.M.
Center for Disease Control
"All products have been great or quickly exchanged."
Reviewed By K.P.
University of Toledo-HSC
"Good variety of clones with good choice of labels"
Reviewed By M.B.
Kirksville College of Osteopathic Medicine
"We have been using OriGene for the last five years. The plasmids are amazing"
Reviewed By S.K.
UT Southwestern Medical Center
"Excellent quality and affordable price"
Reviewed By K.P.
St. Johns University
cDNA Clone Resources