Products
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pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ugC-Tag Myc-DDKE.coli Selection NeomycinMammalian Selection Kanamycin (25 ug/mL)
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TrueRAB®
Rabbit Monoclonal Antibody
Click here to learn more.
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tagsApplications ELISA, FACS, FC, IF, IHC, IP, WBConjugation UnconjugatedImmunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within DDK. The exact sequence is proprietary. -
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Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibodyApplications FC, IF, IP, WBConjugation UnconjugatedImmunogen Anti-DDK monoclonal antibody is produced by immunizing mice with a synthetic peptide coupled to KLH[TA592569&A superior antibody for DDK detection is now available for applications WB, IP, FC, FACS, ELISA]
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Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ugC-Tag TurboGFPE.coli Selection NeomycinMammalian Selection Ampicillin (100 ug/mL)
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Trilencer-27 Universal scrambled negative control siRNA duplex - 2 nmol
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TrueMAB™
Antibodies made against full-length proteins as antigens.
Click here to learn more.
Mouse monoclonal turboGFP antibody, clone OTI2H8Applications IF, IP, WBConjugation UnconjugatedImmunogen Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell. -
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qSTAR qPCR primer pairs against Homo sapiens gene GAPDHComponents 1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
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- Unconjugated (133511)
- Carrier-free
- Knockout (KO) Validation
- Recombinant
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- Secretory
- Expression Host
- Lentivirus
- Tag
-
- C- 6×His (8)
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- C-6His (298)
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- C-Myc/DDK (77561)
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- C-term Flag Tag (1000)
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- DDK-tag (1)
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- His tag (1)
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- His-tag (5790)
- His-Tag (59)
- His-tagged, myc-tagged (3)
- mBFP (1)
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- Myc-DDK (295909)
- Myc-His-tag (2)
- N-10*His (1)
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- N-6His (55)
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- N-6His-Avi (4)
- N-6His-FLAG (1)
- N-6His-Flag (2)
- N-6xHis (1)
- N-6xHis/AVI (2)
- N-6×His (8)
- N-8His (5)
- N-8His&N-FLAG (2)
- N-8His-Flag (1)
- N-AVI (1)
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- N-His (2903)
- N-HIS and C-AVIPLUS (1)
- N-His PDCD1(Pro21-Val170) (5)
- N-His&C-Fc (4)
- N-His&C-His (42)
- N-His&Flag (3)
- N-His&MBP (1)
- N-His&SUMO (10)
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- N-His-GST (4)
- N-His-PDCD1(Pro21-Val170) (2)
- N-His-SUMO (49)
- N-His-Trx (18)
- N-His/DDK (5)
- N-Human Fc (51)
- N-MARI (4)
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- N-mFc (3)
- N-mGFP (13)
- N-Mouse Fc (2)
- N-Mouse Fc and C-6×His (5)
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- N-PROTEIN (1)
- N-T7 (1)
- N-T7&C-His (24)
- N-terminal His (2)
- N-terminal His-PDCD1(Pro21-Val170) tag (2)
- N-terminal His-tag (1)
- N-terminal murine Fc Tag (1)
- N-tGFP (16)
- N-tRFP (14)
- N-TRX (1)
- N-Trx (4)
- N-Trx&His (24)
- N-Trx-His (1)
- N-TRXA (1)
- N-TrxA (2)
- No (1)
- Non-Tagged (6)
- T7-tag (2)
- T7-tag (28)
- Tag Free (92056)
- TrxA (2)
- TurboGFP (65432)
- Vector
-
- EF1a-GFP-P2A-Puro (39257)
- GFP-puro (1378)
- Luciferase-Puro (1377)
- mBFP-Neo (1377)
- pCMV6-AC (3792)
- pCMV6-AC-GFP (65513)
- pCMV6-AC-mBFP (10)
- pCMV6-AC-mGFP (10)
- pCMV6-AC-mRFP (24)
- pCMV6-AC-Myc-DDK (151)
- pCMV6-AC-RFP (11)
- pCMV6-AN-GFP (16)
- pCMV6-AN-mBFP (13)
- pCMV6-AN-mGFP (13)
- pCMV6-AN-mRFP (13)
- pCMV6-AN-Myc-DDK (27)
- pCMV6-AN-RFP (14)
- pCMV6-Entry (152143)
- pCMV6-Entry2 (19)
- PCMV6-Kan/Neo (6167)
- PCMV6-Neo (248)
- pCMV6-XL4 (5168)
- pCMV6-XL5 (11554)
- pCMV6-XL5-DDK-His (76)
- pCMV6-XL6 (791)
- pCMVMIR (3425)
- pGFP-C-shLenti (107886)
- pGFP-V-RS (2316)
- pLenti-C-mGFP (21910)
- pLenti-C-mGFP-P2A-Puro (136774)
- pLenti-C-Myc-DDK (22444)
- pLenti-C-Myc-DDK-P2A-Puro (136950)
- pRFP-C-RS (1373)
- pRMT-tGFP, Promoter-less GFP Reporter Vector (3)
- pRS (53999)
- RFP-BSD (1377)
- Reporter
- Modification
-
- Acetyl K12 (2)
- Acetyl K14 (1)
- Acetyl K15 (1)
- Acetyl K16 (2)
- Acetyl K18 (1)
- Acetyl K23 (1)
- Acetyl K27 (2)
- Acetyl K29 (1)
- Acetyl K332 (1)
- Acetyl K36 (2)
- Acetyl K4 (2)
- Acetyl K5 (4)
- Acetyl K56 (1)
- Acetyl K8 (1)
- Acetyl K9 (1)
- Acetyl K9/K14/K18/K23/K27 (1)
- Acetyl K91 (1)
- Asymmetric DiMethyl R3 (1)
- citrulline R2/R8/R17 (1)
- K27-linkage Specific Polyubiquitin (1)
- K29-linkage Specific Polyubiquitin (1)
- K33-linkage Specific Polyubiquitin (1)
- K6-linkage Specific Polyubiquitin (1)
- M1-linkage Specific Polyubiquitin (1)
- monoMethyl E105 (1)
- MonoMethyl K342 (1)
- MonoMethyl K36 (1)
- MonoMethyl K5 (1)
- Pan DiMethyl-lysine (1)
- pan Phospho-Serine/Threonine (1)
- Pan Phospho-Tyrosine (1)
- pan Symmetric-DiMethyl-Arginine (1)
- pan-Di-Methyl K (1)
- pan-Mono-Methyl K (1)
- pan-Mono-MethylR(R*GG) (1)
- pan-Myristyl-G (1)
- pan-tri-Methyl K (1)
- pan-TriMethyl-lysine (1)
- Phospho Phospho S87 (1)
- Phospho pS221 (1)
- Phospho S1 (1)
- Phospho S10 (1)
- Phospho S1033 (1)
- Phospho S104 (2)
- Phospho S11 (1)
- Phospho S1105 (1)
- Phospho S1108 (1)
- Phospho S1177 (1)
- Phospho S1248 (1)
- Phospho S127 (1)
- Phospho S128 (1)
- Phospho S129 (2)
- Phospho S1387 (1)
- Phospho S139 (2)
- Phospho S1490 (1)
- Phospho S15 (1)
- Phospho S157 (1)
- Phospho S16 (2)
- Phospho S16/T17 (1)
- Phospho S166 (2)
- Phospho S172 (1)
- Phospho S176/180 (1)
- Phospho S180 (1)
- Phospho S182 (1)
- Phospho S189,Phospho S207 (1)
- Phospho S19 (1)
- Phospho S194 (1)
- Phospho S1943 (1)
- Phospho S1981 (1)
- Phospho S2 (1)
- Phospho S20 (1)
- Phospho S2056 (1)
- Phospho S209 (1)
- Phospho S21 (2)
- Phospho S211 (1)
- Phospho S2152 (1)
- Phospho S22 (1)
- Phospho S235/236 (1)
- Phospho S240/244 (1)
- Phospho S241 (1)
- Phospho S243 (1)
- Phospho S2481 (1)
- Phospho S253 (1)
- Phospho S257/T261 (1)
- Phospho S259 (1)
- Phospho S271 (1)
- Phospho S28 (1)
- Phospho S280 (1)
- Phospho S29/33/37/T41 (1)
- Phospho S293 (1)
- Phospho S294 (1)
- Phospho S298 (1)
- Phospho S307 (1)
- Phospho S308,Phospho S309,Phospho S305 (1)
- Phospho S319 (2)
- Phospho S326 (1)
- Phospho S33 (1)
- Phospho S33/S37/T41 (1)
- Phospho S330 (1)
- Phospho S334 (1)
- Phospho S338 (1)
- Phospho S345 (1)
- Phospho S36 (1)
- Phospho S366 (1)
- Phospho S37 (1)
- Phospho S376 (3)
- Phospho S380 (2)
- Phospho S380/T382/383 (1)
- Phospho S39 (1)
- Phospho S392 (1)
- Phospho S396 (2)
- Phospho S397 (1)
- Phospho S40 (1)
- Phospho S409/S410 (1)
- Phospho S412 (1)
- Phospho S420 (1)
- Phospho S422 (1)
- Phospho S423/S425 (1)
- Phospho S425 (1)
- Phospho S428 (1)
- Phospho S445 (1)
- Phospho S448 (1)
- Phospho S45 (1)
- Phospho S455 (1)
- Phospho S46 (1)
- Phospho S465/S467 (1)
- Phospho S467 (1)
- Phospho S471/472 (1)
- Phospho S473 (1)
- Phospho S473,Phospho S474,Phospho S472 (1)
- Phospho S483 (1)
- Phospho S485 (1)
- Phospho S5 (1)
- Phospho S505 (1)
- Phospho S507 (1)
- Phospho S51 (1)
- Phospho S516 (1)
- Phospho S519/T522 (1)
- Phospho S529 (1)
- Phospho S552 (1)
- Phospho S555 (1)
- Phospho S56 (1)
- Phospho S563 (1)
- Phospho S588 (1)
- Phospho S6 (1)
- Phospho S608 (1)
- Phospho S611/615 (1)
- Phospho S611/615/616 (1)
- Phospho S616 (1)
- Phospho S62 (1)
- Phospho S621 (1)
- Phospho S63 (1)
- Phospho S636/639 (1)
- Phospho S638 (1)
- Phospho S641 (1)
- Phospho S643/676 (1)
- Phospho S645 (1)
- Phospho S65 (1)
- Phospho S657/Y658 (1)
- Phospho S668 (1)
- Phospho S675 (1)
- Phospho S7 (1)
- Phospho S70 (1)
- Phospho S71 (1)
- Phospho S727 (2)
- Phospho S728 (1)
- Phospho S73 (1)
- Phospho S744/748 (1)
- Phospho S795 (1)
- Phospho S807/811 (1)
- Phospho S811 (1)
- Phospho S82 (1)
- Phospho S83 (4)
- Phospho S838/S840 (1)
- Phospho S886 (1)
- Phospho S89 (1)
- Phospho S890 (1)
- Phospho S896/S897 (1)
- Phospho S897 (1)
- Phospho S9 (2)
- Phospho S916 (1)
- Phospho S957 (1)
- Phospho S967 (1)
- Phospho Ser15 (1)
- Phospho Ser404 (1)
- Phospho Ser416 (1)
- Phospho Ser792 (1)
- Phospho Ser90 (1)
- Phospho T11 (1)
- Phospho T117 (1)
- Phospho T14 (1)
- Phospho T1462 (1)
- Phospho T156 (1)
- Phospho T163 (1)
- Phospho T17 (1)
- Phospho T172 (1)
- Phospho T179 (1)
- Phospho T18/S19 (1)
- Phospho T180/Y182 (1)
- Phospho T180/Y182 + T183/Y1845 (1)
- Phospho T181 (1)
- Phospho T183/Y185 (1)
- Phospho T183/Y185,Phospho T221/Y223 (1)
- Phospho T186 (1)
- Phospho T189 (1)
- Phospho T197 (1)
- Phospho T1989 (1)
- Phospho T199 (1)
- Phospho T202/Y204 + T185/Y187 (1)
- Phospho T204 (1)
- Phospho T217 (1)
- Phospho T231 (1)
- Phospho T231/S232 (1)
- Phospho T232 (1)
- Phospho T235 (1)
- Phospho T24,Phospho T32,Phospho T28 (1)
- Phospho T246 (1)
- Phospho T261 (1)
- Phospho T286 (2)
- Phospho T288 (1)
- Phospho T288,Phospho T232,Phospho T198 (1)
- Phospho T292 (1)
- Phospho T3 (1)
- Phospho T308 (1)
- Phospho T32 (1)
- Phospho T320 (1)
- Phospho T334 (1)
- Phospho T346 (1)
- Phospho T357/S358/S360 (1)
- Phospho T359 (1)
- Phospho T359/S363 (1)
- Phospho T384 (1)
- Phospho T387 (1)
- Phospho T389 (2)
- Phospho T395 (1)
- Phospho T410 (1)
- Phospho T421/S424 (1)
- Phospho T434 (1)
- Phospho T446 (1)
- Phospho T45 (1)
- Phospho T498 (1)
- Phospho T505 (1)
- Phospho T514 (2)
- Phospho T538 (1)
- Phospho T56 (1)
- Phospho T567,Phospho T564,Phospho T558 (1)
- Phospho T58 (1)
- Phospho T642 (1)
- Phospho T668 (1)
- Phospho T669 (1)
- Phospho T69/71 (1)
- Phospho T69/T71 (1)
- Phospho T696 (1)
- Phospho T70 (1)
- Phospho T81 (1)
- Phospho T853 (1)
- Phospho T91 (1)
- Phospho T918 (1)
- Phospho T93 (1)
- Phospho T982 (1)
- Phospho Thr212/Ser214 (1)
- Phospho Y1007 (1)
- Phospho Y1007/1008 (1)
- Phospho Y1008 (1)
- Phospho Y1020 (1)
- Phospho Y1021 (1)
- Phospho Y1022/1023 (1)
- Phospho Y1045 (1)
- Phospho Y1054/1055 (1)
- Phospho Y1086 (1)
- Phospho Y1096 (1)
- Phospho Y1148 (1)
- Phospho Y1165/1166,Phospho Y1189/1190 (1)
- Phospho Y1173 (1)
- Phospho Y1175 (1)
- Phospho Y1189/Y1190,Phospho Y1165/Y1166 (1)
- Phospho Y1217 (1)
- Phospho Y1221/1222 (1)
- Phospho Y1234/1235 (1)
- Phospho Y1284 (1)
- Phospho Y133 (1)
- Phospho Y1349 (1)
- Phospho Y14 (1)
- Phospho Y145 (1)
- Phospho Y1474 (1)
- Phospho Y15 (1)
- Phospho Y165 (1)
- Phospho Y171 (1)
- Phospho Y177 (1)
- Phospho Y182 (1)
- Phospho Y191 (1)
- Phospho Y207 (1)
- Phospho Y216 (1)
- Phospho Y221 (1)
- Phospho Y245 (1)
- Phospho Y284 (1)
- Phospho Y307 (1)
- Phospho Y311 (1)
- Phospho Y340 (1)
- Phospho Y353 (1)
- Phospho Y410 (2)
- Phospho Y412 (1)
- Phospho Y416 (1)
- Phospho Y416/Y416 (1)
- Phospho Y467/Y199/Y464 (1)
- Phospho Y564 (1)
- Phospho Y576/577 (1)
- Phospho Y589/591 (1)
- Phospho Y594 (1)
- Phospho Y653 (1)
- Phospho Y659 (1)
- Phospho Y674/675 + Y706/707 (1)
- Phospho Y694 (1)
- Phospho Y700 (1)
- Phospho Y705 (1)
- Phospho Y708 (1)
- Phospho Y719 (1)
- Phospho Y724 (1)
- Phospho Y751 (1)
- Phospho Y754 (1)
- Phospho Y759 (1)
- Phospho Y789 (1)
- Phospho Y792 (1)
- Phospho Y845 (1)
- Phospho Y849 (1)
- Phospho Y869 (1)
- Phospho Y925 (1)
- Phospho Y980/981 (1)
- Phospho Y992 (1)
- Phospho Y996 (1)
- Phospho Y998 (1)
- Phospho-(Ser/Thr) (1)
- Phospho-PKC (pan) (βII Ser660) (1)
- Phospho-RPS6KB1-S371 (1)
- Phospho-specific (1282)
- succinylation K79 (1)
- This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. (1)
- This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. (1)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. (5)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques. (1)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques. (1)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. (22)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques. (7)
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques. (9)
- TriMethyl K56 (2)
- Ubiquityl K119 (1)
- Unconjugated (1)
- Unmodified (6969)
- Protein Source
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- tYFP (1)
- Mammalian Cell Selection
- Viral Packaging
- Protein Families
-
- 0.003% (w/v) bovine serum.2. Add recombinant ADA protein with various concentrations (0.1ug (2)
- 0.003% (w/v) BSA (4)
- 0.005 units xanthine oxidase.2. Equilibrate to 25°C and monitor at A550nm until the value is constant using a spectrophotometer.3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug (2)
- 0.01 mM cytochrom C (2)
- 0.03 ug) in assay buffer and equilibrate to 37°C for 30 minutes.(Assay buffer: 50 mM Tris-HCl (2)
- 0.033% (w/v) bovine serum albumin.2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer3. Add 50ul of recombinant LDHA protein in various concentrations (0.1ug (2)
- 0.03X PBS (10)
- 0.045mM adenosine (2)
- 0.05 mM xanthine (2)
- 0.05ug) in 180ul reaction buffer. 4. Mix by inversion and record the decrease at A340nm for 10 minutes. (2)
- 0.065 mM beta-nicotinamide adenine dinucleotide (2)
- 0.075 ug and 0.15 ug in assay buffer3. Mix and load 200 ul of reaction mix in to a plate well. 4. Record the decrease in A340nm for 5 minutes at 25°C. (2)
- 0.09mM beta-NADPH (2)
- 0.1 mg/ml Ovalbumin (6)
- 0.1 mM ethylendiaminetetraacetic acid (2)
- 0.1% (w/v) insulin (4)
- 0.12 mM beta-nicotinamide adenine dinucleotide (2)
- 0.125ug to each well.4. Record the decrease in A260nm for 5 minutes at 25°C using UV plate. (2)
- 0.13 mM beta-nicotinamide adenine dinucleotide (4)
- 0.1ug (2)
- 0.2) in assay buffer.3. Mix by inversion and record A260nm for approximately 5 minutes. (2)
- 0.25 mM oxalacetic acid.2. Add recombinant MDH protein with various concentrations (0.1ug (4)
- 0.3mM NADPH.2. Add 50ul of recombinant AKR1C4 solution with various concentrations (1ug (2)
- 0.3mM NADPH.2. Add 50ul of recombinant AKR1D1 protein solution with various concentrations (1ug (2)
- 0.44 mM DL-isocitric acid (2)
- 0.5 mM EDTA (6)
- 0.5% glycerol.2. Add 5 ul of recombinant PRDX2 solution with various concentrations (0.25ug (2)
- 0.5% glycerol.2. Add 5 ul of recombinant PRDX3 solution with various concentrations (0.25ug (2)
- 0.5% glycerol.2. Add 5 ul of recombinant PRDX4 solution with various concentrations (0.25ug (2)
- 0.5% glycerol.2. Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug (2)
- 0.5% glycerol.2. Add 5 µl of recombinant PRDX1 solution with various concentrations (0.25 µg (2)
- 0.5ug (4)
- 0.5ug) in assay buffer.4. Mix by inversion and record the increase at A340nm for 5 minutes. (2)
- 0.6 mM manganese chloride. 2. Add 50ul of recombinant protein IDH1 with 1 ug (2)
- 0.74mM ATP (4)
- 0.9 mM Xanthine (2)
- 0.9 ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl (2)
- 0.95mM fructose 1 (2)
- 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl (2)
- 0.9ng) in assay buffer and equilibrate to 37°C for 10 minutes. (Assay buffer: 50mM Tris-HCl (2)
- 000 (1)
- 000 - 1/10 (1)
- 000 - 1/5 (1)
- 000 - 1/50 (1)
- 000 and 50 (1)
- 000 is suggested for this product. (1)
- 000 of the stock concentration is suggested for this product. (2)
- 000 or dot blot) (1)
- 000 to 1/3 (1)
- 000) (3)
- 000) and Immunohistochemistry (1/200-1/1 (2)
- 000) as well as other antibody based fluorescent assays. (1)
- 000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays. (1)
- 000) as well as other Glucose Oxidase-biotin-avidin based enzymatic assays.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin. (1)
- 000) as well as other peroxidase-biotin-avidin based enzymatic assays. (2)
- 000) as well as other phosphatase-avidin/biotin based enzymatic assays. (1)
- 000) as well as other phosphatase-Biotin/biotin based enzymatic assays.Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin. (1)
- 000)and Immunohistochemistry (1:200-1:1 (1)
- 000)Flow Cytometry/FACS (1/1 (1)
- 000-1/10 (1)
- 000-1/200 (5)
- 000-1/32 (5)
- 000-1/4 (1)
- 000-1/40 (5)
- 000-1/5 (12)
- 000-1/50 (5)
- 000-1:32 (1)
- 000. (8)
- 000. Flow Cytometry: 1/500-1/2 (1)
- 000. Immunofluorescence: 1/1 (1)
- 000. Immunohistochemistry: 1/1 (1)
- 000. Western Blot: 1/10 (1)
- 000.Fluorescent Western blotting. (1)
- 000.Immunofluorescence: 1/1 (1)
- 000.Flow Cytometry: 1/500-1/2 (3)
- 000.IF Microscopy: 1/1 (2)
- 000.Immunochemistry: 1/1 (1)
- 000.Immunohistochemistry: 1/1 (2)
- 000.Immunohistochemistry: 1/200-1/1 (1)
- 000.Western blot: 1/1 (1)
- 000.Western Blot: 1/10 (1)
- 000.Western blot: 1/10 (1)
- 000.Western Blot: 1/2 (1)
- 000; This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using ABTS (2 (1)
- 000Immunofluorescence: 1/1 (1)
- 1 mM DTT (6)
- 1 ug) and read the increase in A340nm for 5 minutes. (2)
- 1 unit glucose-6-phosphate dehydrogenase (4)
- 1.0 mM beta- NADP (2)
- 1.1 mM beta-NADP (4)
- 1/10 (1)
- 1/500-1/2 (1)
- 10 mM 2-mercaptoethanol (2)
- 10 mM DTT (2)
- 10 mM glucose (4)
- 10 mM magnesium chloride (4)
- 10 mM sodium phosphate (2)
- 10 mM Tris-HCl (18)
- 100 mM potassium chloride (2)
- 10mM DL-glyceraldehyde (4)
- 10mM pNPP.2. Pre-warm reaction mix in heat block at 37°C.3. Add recombinant PPP1CA solution with various concentrations (2ug (2)
- 10mM Tris-HCl (2)
- 10ug/ml (2)
- 15mM MgCl2 (2)
- 15mM MgCl2. 2. Dilute IMPAD1 various concentration (20ug/ml (2)
- 1:500-1:2 (1)
- 1mM DTT (4)
- 1mM glutathione (2)
- 1mM MnCl2 (2)
- 1ug (2)
- 1ug) and read the increase in A340nm for 5 minutes. (2)
- 1ug) in assay buffer.3. Mix by inversion (2)
- 1ug) in assay buffer.3. Mix by inversion and equilibrate to 1°C and monitor at A340nm until the value is constant using a spectrophotometer.4. Record the increase at A340nm for approximately 2 minutes. (2)
- 1ug) in assay buffer.4. Mix by inversion and record the increase at A550nm for 5 minutes. (2)
- 2 mM dithiothreitol (2)
- 2 mM acetaldehyde2. Equilibrate to 25°C and monitor the A340nm until value is constant using a spectrophotometer.3. Add 20 ul of recombinant ALDH2 protein 400ug/ml in assay buffer.4. Record the increase in A340nm for 5 minutes. (2)
- 2 mM beta-NAD (2)
- 2 mM dithiothreitol (18)
- 2 ug (2)
- 2 unit hexokinase (4)
- 2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature) (1)
- 2.0 mM adenosine 5'-diphosphate (4)
- 2.3 mM beta-nicotinamide adenine dinucleotide phosphate (4)
- 2.3 mM Pyruvate (2)
- 2.5M KSCN)7. Record the increase in A475nm. (8)
- 2.5M KSCN)7. Record the increase in A475nm. (2)
- 2.6mM EDTA (2)
- 216 mM D-glucose (4)
- 2375mM Glycerol (2)
- 2mM dithiothreitol (2)
- 2mM DTT (2)
- 2mM EDTA.2. Add 4 ug of recombinant thioredoxin2 into reaction cocktail and mix immediately.3. Incubate at 25°C for 20 minutes.4. Record the increase in A650nm for 15 minutes. (2)
- 2mM EDTA.2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.3. Incubate at 25°C for 20 minutes.4. Record the increase in A650nm for 15 minutes (2)
- 2ug in reaction mixture. 3. Record the decrease in A340nm for 5 minutes at 25°C. (2)
- 2ug) in 750ul reaction buffer.3. Mix by inversion and incubate at 25°C for 2.5 minutes.4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.5. Record the increase at A340nm for 3 minutes. (2)
- 2ug) in assay buffer.3. Record the increase in A260 nm for 10 minutes- L-Malate (Sigma-Aldrich. Cat. No (2)
- 3635)- plate reader (PerkinElmer (2)
- 3mM b-NAD. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 20ul of recombinant gldA protein with various concentrations (0.2ug (2)
- 3ug) in 200ul reaction buffer.4. Mix by inversion and incubate at 37°C for 10 minutes.5. Stop the reaction by adding the 800ul of 0.1N NaOH.6. Record the absorbance at 405nm. (2)
- 450 ul assay buffer. The final concentrations are 75mM Potassium phosphate (pH 7.5) (2)
- 5 mM EDTA (2)
- 5 unit alpha-glcerophosphate dehydrogenase/triosephosphate isomerase. 2. Add 50 ul of recombinant Aldolase A protein with 1ug (2)
- 5.5 mM Ethylendiaminetetraacetic acid (2)
- 50 mM L-malic acid).2. Add 50 ul of recombinant Fumarase protein with various concentrations (0.5 ug (2)
- 500 or dot blot) (1)
- 500 or Dot blot) (1)
- 500 to 1:19 (1)
- 500) (3)
- 500. (3)
- 500. FACS analysis as well as other antibody based fluorescent assays. (1)
- 50mM NaCl (2)
- 5ug/ml) in 20mM Tris-HCl (PH 7.5) (2)
- 6-diphosphate (2)
- 63mM sodium phosphate (4)
- 655077) - Fluorescent plate reader (PerkinElmer (2)
- 655077)- Fluorescent plate reader (PerkinElmer (6)
- 66 mM Cytochrom-C (2)
- 7.8 mM MgCl2 (4)
- 93mM Potassium chloride (2)
- ATPase Activity Assay (3)
- Surface Plasmon Resonance (SPR). Specificity: ~90 kDa (3)
- Rat IgG F(c) Fragment can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. (1)
- ATPase Assay.Western B Control.Binding Assays.ELISA reference standard.Lipid Interaction Assays. (3)
- Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml (3)
- ELISA (0.5 µg/ml).Western blot. (1)
- ELISA and Western blot.Using an antigen Capture ELISA (1)
- ELISA. Lateral Flow. (2)
- ELISA. (8)
- ELISA. Western blot. (1)
- ELISA.Functional Assays. (2)
- ELISA.Western Blot. (4)
- ELISA.Western Blot.Cell culture and/or animal studies. (1)
- ELISA.Western Blot.Lateral Flow. (1)
- ELISA.Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.Immunoprecipitation.Western blotting. (1)
- ELISA.Western Blot. (3)
- ELISA.Western blotting. (1)
- ELISA: 0.6-1.25 µg/ml. Gel Super Shift Assays. (1)
- ELISA: SA1000 May be used as a standard with SM2216P or SM2228B.Functional Assays. (1)
- ELISA: When coating use 10 ng-1 µg/well. (1)
- FLISA: 1/10 (2)
- Functional Assays. (4)
- Positive control for Western blot analysis (1)
- Positive control for Western blot analysis.Standard for ELISA. (1)
- Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.4. Lauber T (1)
- adjust to pH 7.5 at 37°C and pre-chill on ice before use: The final concentrations are 100 mM Sodium phosphate (2)
- Adult stem cells (3902)
- and 0.13% BSA. 2. Add 50 ul of recombinant gor protein with 0.037 ug (2)
- and 5 ug in assay buffer. 3. Mix by inversion and load 200 ul of reaction mix in to a plate well. chloride. 4. Record the increase in A340 nm for 5 minutes at 25C. (2)
- and Fukazawa H. (1991) Methods Enzymol. 201: 370.2. Fukazawa H. (2)
- and monitor at A340nm until the value is constant using a spectrophotometer.4. Record the increase at A340nm for approximately 2 minutes. (2)
- and Nuccitelli R. (1992) J.Biol.Chem. 267:5608. (2)
- and therefore gives very low backgrounds. (1)
- anti-Goat IgG (1)
- anti-Goat IgG F(c) and anti-Goat Serum.No reaction was observed against anti-Goat IgG F(ab')2 or anti-Papain. (1)
- anti-Human Albumin and anti-Human Serum. (2)
- anti-Rabbit IgG (1)
- anti-Rabbit IgG F(c) and anti-Rabbit Serum.No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain. (1)
- anti-Rat IgG (1)
- anti-Rat IgG F(c) and anti-Rat Serum.No reaction was observed against anti-Rat IgG F(ab')2 or anti-Papain. (1)
- as a control or standard in assays (11)
- as a Control or Standard in assays (1)
- as a Control or standard in assays (1)
- ATPase Assay (6)
- ATPase AssayWestern Blot Control.Binding Assays.ELISA reference standard. (3)
- Binding Assays (6)
- Bioactivity (39)
- black (greiner bio-one (8)
- BML-P236-0025)- 96 Well Polystyrene Microplate (2)
- BML-SE211)- 96 Well Polystyrene Microplate (4)
- BML-SE211)- 96 Well Polystyrene Microplate (2)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (2)
- both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl (12)
- Bovine (2)
- Can be used as standard in a Sandwich ELISA. (2)
- Cancer stem cells (1984)
- Cat. No (2)
- Cat.No (8)
- Cat.No. H-8629)2. Add 50 ul of recombinant Adenylate kinase isoenzyme 2 protein with various concentrations (0.5 ug (2)
- Cat.No. H-8629)2. Add 50 ul of recombinant AK1 protein with various concentrations (0.5ug (2)
- Cell culture and/or animal studies.ELISA.Western Blot. (1)
- change growth medium against basal medium (EBM) in the early afternoon]- change EGF against EBM (for HUVEC: EBM +1-2% FCS)- incubate 24h - change medium again and add factors (growth factors (2)
- Chaperone assays. (3)
- Citr ic acid 0.055 g (2)
- Citric acid 0.055 g (6)
- Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.3. Schulz A (1)
- Colloidal Gold and Latex Beads. (2)
- containing blank of 20mM Tris-HCl (2)
- Druggable Genome (213551)
- e.g. Dulbecco's PBS). (12)
- e.g. Dulbeccos PBS). (4)
- e.g. Dulbeccos PBS).See References 2 and 3 for more details. (4)
- e.g.Dulbecco's PBS). (2)
- each lot is tested against a reference material for both IGM and IgG activity. (1)
- ELISA (221)
- ELISA (1/100 (1)
- ELISA (1/20 (2)
- ELISA (1/8 (3)
- ELISA (1:8 (1)
- ELISA : 1/20 (1)
- ELISA and Immunohistochemistry (1:200- 1:1 (1)
- ELISA and Western blot. (2)
- ELISA and Western blot.Glycosylation is heterogeneous and results in several immunoreactive products on Western blots that range from approximately 40 (1)
- ELISA reference standard (3)
- ELISA reference standard.This protein has ATPase activity at the time of manufacture of 3.2 uM phosphate liberated/hr/ug protein in a 200 uL reaction at 37 C (pH 7.5) in the presence of 20 uL of 1 mM ATP using a Malachite Green assay. (3)
- ELISA. (30)
- ELISA.Functional Assays. (2)
- ELISA.Western blot. (1)
- ELISA.Western Blot. (18)
- ELISA.Western blot.Lateral flow. (2)
- ELISA.Western blotting. (2)
- ELISA.Western Blotting. (1)
- ELISA: 0.1-1.25 µg/ml. (1)
- ELISA: 0.5-1.0 µg/ml coating concentration.After thawing (2)
- ELISA: 1.25µg/ml. (2)
- ELISA: 1/200 (1)
- ELISA: 1/8 (1)
- Embryonic stem cells (2970)
- ensuring maximum specific IgG binding. (1)
- Enzyme Activity (12)
- enzyme purification or to remove unwanted cross-reactivity. (1)
- equilibrate to 1°C (2)
- ES Cell Differentiation/IPS (16565)
- et al. (1991) Biochem. Pharmacol. 42: 1661.3. Satoh T. (2)
- et al. (2003) Dev. Biol. 258:432-442.Heo I. (2)
- et al. (2009) Cell. 138:696-708. (2)
- et al.(1992) J.Biol.Chem. 267: 2537.4. Weiss R. (2)
- FACS (104)
- Flow Cyt (11)
- Flow Cytometry. (1)
- Flow Cytometry.Immunofluorescence. (11)
- Flow Cytometry: Use 10µl of the suggested working dilution to label 10e6 cells or 100µl of whole blood. (1)
- fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis (1)
- Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis (1)
- Fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis (1)
- fluorescence based plate assays (FLISA) and Fluorescent Western blotting.This product is also suitable for multiplex analysis (1)
- for conjugation and for most other immunological methods. This recombinant Protein G contains only IgG binding domains (1)
- for conjugation and most other immunological methods requiring highly purified immunoglobulins. (10)
- for conjugation and most other immunological methods. (1)
- for Immunoprecipitation and for other immunological assays optimized by the customer. (1)
- for Immunoprecipitation and for Western blotting (2)
- for Immunoprecipitation and for Western blotting. (5)
- for immunoprecipitation and for Wwestern blotting. (1)
- for quantitation of antigen in serum using a standard curve (1)
- For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.Immunoprecipitation.Western blotting. (1)
- For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve (1)
- Forssmann WG (1)
- Functional Assays. (1)
- Functional assays. (1)
- Functional Assays.In vitro Assay. (1)
- GPCR (7400)
- GPI-anchored protein (2)
- Hawkins JA (1)
- Hidaka Y (1)
- IHC (1)
- immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin (1)
- Immuno-microscopy as well as other antibody based assays using streptavidin or avidin conjugates.Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin (1)
- immuno-peroxidase electron microscopy and Immunohistochemistry (1/1 (1)
- Immunoassay. (1)
- Immunoblotting (2)
- Immunoblotting.ELISA.Immunohistochemistry.Immunofluorescence. (1)
- Immunoblotting.ELISA.Immunohistochemistry.Immunomicroscopy.as well as other antibody based assays using streptavidin or avidin conjugates. (1)
- Immunofluorescence Microscopy (1/1 (1)
- Immunofluorescence.Flow Cytometry. (8)
- Immunogen (2)
- immunogen for antibody production. (4)
- immunogen for antibody production.Has been tested with MAb to Influenza A in ELISA. (4)
- Immunohistochemistry (2)
- immunohistochemistry (2)
- immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. (1)
- Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin (1)
- Immunomicroscopy as well as other antibodybased assays using streptavidin or avidin conjugates.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin (1)
- immunoperoxidase electron microscopy and immunochemistry (1/1 (1)
- Immunoperoxidase Electron Microscopy and Immunohistochemistry (1/1 (1)
- immunoperoxidase electron microscopy and immunohistochemistry (1/200-1/1 (1)
- Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase (1)
- Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.Recommended Dilutions:ELISA: 1/20 (1)
- Immunoprecipitation. (1)
- In vitro Assay.Functional Assays. (1)
- including multicolor imaging (4)
- Induced pluripotent stem cells (1654)
- inhibitors (3)
- Ion Channels: ATP Receptors (204)
- Ion Channels: Calcium (255)
- Ion Channels: Cyclic nucleotide gated (130)
- Ion Channels: Cys-loop Receptors (960)
- Ion Channels: Glutamate Receptors (483)
- Ion Channels: Other (3313)
- Ion Channels: Potassium (1581)
- Ion Channels: Sodium (248)
- Ion Channels: Transient receptor potential (677)
- Lipid Interaction Assays (3)
- Low Endotoxin Control Rabbit IgG can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. (1)
- M-1000)- 96 well UV plate (Costar (2)
- Malachite green carbinol hydrochloride 3.3 mg in 50 ml of 1M H2SO4. (2)
- Marx UC (1)
- Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.2. Rudolph JA (1)
- Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21. (1)
- mix thoroughly by vortexing before use. (2)
- Neudecker P (1)
- Nourse A (1)
- Nuclear Hormone Receptor (3053)
- optimal concentration with heparin (30 μg/ml) is about 12 μg/ml. (2)
- or Dot blot) (1)
- P450 (1395)
- pH 7.4). For immunization purposes (4)
- pH 7.4). For immunization purposes (8)
- pH 7.4).For immunization purposes (6)
- pH 7.4).For Immunization purposes (4)