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ZNF703 (NM_025069) Human Tagged ORF Clone

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SKU
RC207466
ZNF703 (Myc-DDK-tagged)-Human zinc finger protein 703 (ZNF703)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $549.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF703
Synonyms NLZ1; ZEPPO1; ZNF503L; ZPO1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207466 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGATTCGCCCGCTGGATCTAACCCAAGGACACCCGAAAGCAGCGGCAGCGGCAGCGGCGGCGGCG
GGAAGAGGCCGGCGGTGCCGGCAGCGGTGTCCCTCTTGCCACCGGCGGACCCCCTGCGCCAGGCGAACCG
GCTCCCGATCAGGGTCCTGAAGATGCTGAGCGCTCACACCGGTCACCTCCTGCACCCGGAGTACCTGCAG
CCGCTGTCCTCCACTCCCGTCAGCCCCATTGAGCTGGACGCCAAGAAGAGCCCCTTGGCGCTGCTGGCTC
AGACCTGCTCGCAGATCGGCAAGCCGGACCCGCCGCCCTCCTCCAAACTCAACTCGGTGGCGGCGGCGGC
CAACGGGCTGGGAGCGGAGAAGGACCCCGGCCGCTCAGCCCCGGGCGCCGCCTCCGCAGCCGCGGCCCTG
AAGCAGCTGGGGGACTCACCGGCCGAGGACAAGTCCAGCTTCAAGCCCTACTCCAAGGGCTCCGGCGGCG
GCGACTCCCGCAAAGACAGCGGCTCCTCCTCGGTGTCTTCCACCTCCTCCTCGTCCTCCTCGTCCCCGGG
AGACAAGGCGGGCTTCAGGGTCCCCAGCGCCGCCTGCCCGCCCTTTCCCCCGCATGGAGCGCCGGTCTCC
GCATCCTCGTCCTCGTCGTCGCCCGGCGGCTCCCGCGGCGGCTCCCCGCACCACTCTGACTGCAAGAACG
GCGGCGGGGTTGGCGGCGGGGAGCTGGACAAGAAAGACCAGGAGCCCAAGCCCAGCCCGGAGCCGGCAGC
CGTGAGCCGCGGCGGCGGTGGGGAGCCCGGGGCGCACGGTGGCGCCGAGTCCGGGGCCTCCGGGCGCAAG
TCCGAGCCGCCCTCGGCGCTGGTGGGGGCCGGCCACGTGGCGCCGGTGTCTCCCTACAAGCCGGGCCACT
CGGTGTTCCCGCTGCCGCCCTCCAGCATTGGCTACCACGGCTCCATCGTGGGCGCCTACGCCGGCTACCC
GTCTCAGTTCGTGCCTGGCCTGGATCCTAGCAAGTCCGGCCTCGTGGGAGGCCAGCTGTCTGGGGGCCTG
GGCCTGCCGCCGGGCAAGCCCCCCAGCTCCAGCCCGCTCACCGGGGCCTCCCCGCCCTCCTTCCTGCAGG
GATTATGCCGCGACCCCTATTGCTTGGGAGGTTACCACGGCGCCTCGCACCTCGGCGGCTCCAGCTGCTC
CACCTGCAGCGCGCACGACCCTGCCGGGCCCAGCCTGAAGGCGGGGGGCTACCCGCTGGTGTACCCCGGG
CACCCGCTGCAGCCCGCCGCGCTCTCGTCCAGCGCCGCCCAGGCCGCGCTCCCCGGCCACCCGCTCTACA
CCTACGGCTTCATGCTGCAGAACGAACCGCTGCCGCACAGCTGCAACTGGGTGGCAGCCAGTGGGCCGTG
CGACAAGCGCTTCGCCACCTCGGAGGAGCTGCTCAGCCACCTACGGACCCACACGGCCCTGCCGGGAGCC
GAGAAACTTCTGGCCGCCTACCCCGGGGCCTCGGGCCTGGGCAGCGCCGCCGCCGCCGCCGCCGCCGCCG
CCTCCTGCCATCTGCACCTCCCCCCGCCCGCCGCCCCCGGCAGCCCCGGGTCGCTGTCCTTGCGGAATCC
ACACACTTTGGGCCTAAGCCGGTACCACCCCTATGGCAAGAGCCACTTATCCACAGCGGGGGGCCTGGCC
GTGCCGTCCCTCCCCACAGCCGGACCCTACTATTCGCCATACGCGCTGTATGGACAGAGACTAGCTTCAG
CCTCGGCGCTGGGATACCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207466 protein sequence
Red=Cloning site Green=Tags(s)

MSDSPAGSNPRTPESSGSGSGGGGKRPAVPAAVSLLPPADPLRQANRLPIRVLKMLSAHTGHLLHPEYLQ
PLSSTPVSPIELDAKKSPLALLAQTCSQIGKPDPPPSSKLNSVAAAANGLGAEKDPGRSAPGAASAAAAL
KQLGDSPAEDKSSFKPYSKGSGGGDSRKDSGSSSVSSTSSSSSSSPGDKAGFRVPSAACPPFPPHGAPVS
ASSSSSSPGGSRGGSPHHSDCKNGGGVGGGELDKKDQEPKPSPEPAAVSRGGGGEPGAHGGAESGASGRK
SEPPSALVGAGHVAPVSPYKPGHSVFPLPPSSIGYHGSIVGAYAGYPSQFVPGLDPSKSGLVGGQLSGGL
GLPPGKPPSSSPLTGASPPSFLQGLCRDPYCLGGYHGASHLGGSSCSTCSAHDPAGPSLKAGGYPLVYPG
HPLQPAALSSSAAQAALPGHPLYTYGFMLQNEPLPHSCNWVAASGPCDKRFATSEELLSHLRTHTALPGA
EKLLAAYPGASGLGSAAAAAAAAASCHLHLPPPAAPGSPGSLSLRNPHTLGLSRYHPYGKSHLSTAGGLA
VPSLPTAGPYYSPYALYGQRLASASALGYQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025069
ORF Size 1770 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025069.3
RefSeq Size 2174 bp
RefSeq ORF 1773 bp
Locus ID 80139
UniProt ID Q9H7S9
Cytogenetics 8p11.23
MW 58.2 kDa
Summary Transcriptional corepressor which does not bind directly to DNA and may regulate transcription through recruitment of histone deacetylases to gene promoters. Regulates cell adhesion, migration and proliferation. May be required for segmental gene expression during hindbrain development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025069" in other vectors (6)
SKU Description Size Price
RC207466L1 Lenti ORF clone of Human zinc finger protein 703 (ZNF703), Myc-DDK-tagged 10 ug
$849.00
RC207466L2 Lenti ORF clone of Human zinc finger protein 703 (ZNF703), mGFP tagged 10 ug
$849.00
RC207466L3 Lenti ORF clone of Human zinc finger protein 703 (ZNF703), Myc-DDK-tagged 10 ug
$849.00
RC207466L4 Lenti ORF clone of Human zinc finger protein 703 (ZNF703), mGFP tagged 10 ug
$849.00
RG207466 ZNF703 (tGFP-tagged) - Human zinc finger protein 703 (ZNF703) 10 ug
$749.00
SC122980 ZNF703 (untagged)-Human zinc finger protein 703 (ZNF703) 10 ug
$550.00

Plasmid Map for RC207466

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC207466
Circular map for RC207466

file-alt Citations

Luminal Breast Cancer Cell Lines Overexpressing ZNF703 Are Resistant to Tamoxifen through Activation of Akt/mTOR Signaling
Zhang, Mu, Huang et al
PLoS ONE (2013) 8 (8), e72053 DOI: 10.1371/journal.pone.0072053
Cited by 51
AI Summary: OriGene products contributed to this paper by providing the pCMV6–myc-DDK and pCMV6–myc-DDK-ZNF703 plasmids, which were used to generate stable ZNF703-overexpressing MCF-7 breast cancer cell lines for functional studies on tamoxifen resistance.
Snippet: Essential Media (Gibco) supplemented with 10% FBS. ZNF703 Stable Cell Lines. The pCMV6–myc-DDK and pCMV6–myc-DDK-ZNF703 plasmids were purchased from Origene Company. For stable transfections, MCF-7 cells
FigureFigureFigure
Showing 1-1 of 1 papers.
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