pCMV6-Entry Mammalian Expression Vector

SKU
PS100001
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
  $670.00
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Specifications
Specifications
Product Data
C-Tag Myc-DDK
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Expression Promoter CMV
Features
  • ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal Myc-DDK tags. (DDK is the same as FLAG® which is a registered trademark of Sigma Aldrich)
  • Such clones are the best for detection and purification of the transgene using anti-Myc or anti-DDK antibodies
  • Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms
  • V2_F Primer can be used to sequence the target sequence after cloning
  • XL39_R Primer can be used to sequence the target sequence after cloning
Download Vector Sequence
Schematic of the multiple cloning sites
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Documents
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Blocking lipid raft-associated RANK/c-Src signaling inhibits TRAF6-mediated non-small cell lung cancer migration
Zhou, Ying, Liu et al
Biochim Biophys Acta Gen Subj (2026)
AI Summary: OriGene products contributed to this study by providing the pCMV6 vector (PS100001) used to construct the TRAF6 overexpression plasmid and the pCMV6 empty vector for control experiments.
Snippet: TRAF6 was amplified and inserted into the pCMV6 vector (PS100001, OriGene, USA) to obtain pCMV6-TRAF6 overexpression plasmid, and pCMV6 empty vector was
Radiation-induced SLAMF1 reconfigures the SLAM family receptors landscape in triple-negative breast cancer cells
Song, Park, Jung et al
J Cancer (2026) 17 (10), 1773-1782 DOI: 10.7150/jca.137668
AI Summary: In this study, OriGene’s lentiviral vector (pCMV6-Entry, PS100001) was used to transfect triple-negative breast cancer cells for SLAMF1 overexpression, enabling the investigation of radiation-induced changes in SLAM family receptor expression.
Snippet: rez-nucleotide" xlink:href="PS100001">PS100001, pCMV6-Entry, mammalian expression vector) was transfected into human breast cancer cell MDA-MB-231 and MDA-MB-468 using a lentivirus vector (Origene, Rockville, MD, USA). Briefly, 10 × 104 cells were seeded into a 60 mm dish and medium containing Lipofectamine 3000 (Thermo Fisher Scientific) and a lentiviral vector was added th
FigureFigureFigure
Rejuvenating endogenous antitumor immunity via a chimeric receptor-engineered oncolytic virus targeting tumor-associated macrophages
Lin, Li, Lou et al
Sci Adv (2026) 12 (38), eaef6767 DOI: 10.1126/sciadv.aef6767
AI Summary: OriGene’s pCMV6 expression vector (PS100001) was used to clone cDNAs encoding the extracellular domain of PD-L1 and the αPD-L1 sequence, enabling the construction and functional analysis of chimeric receptors in this study.
Snippet: … The cDNA encoding for the extracellular domain (ECD) of PD-L1 and the αPD-L1 sequence were synthesized and separately cloned into the pCMV6 expression vector (OriGene, PS100001), each tagged with a C-terminal hIgG or His tag, respectiv;- encoding for the extracellular domain (ECD) of PD-L1 and the αPD-L1 sequence were synthesized and separately cloned into the pCMV6 expression vector (OriGene,
FigureFigureFigure
TRPV4 is Associated with a Marfan Syndrome-Related Mechanosensitive Gene Program in Aortic Smooth Muscle Cells
Liu, Bai, Gao et al
Biochem Genet (2026)
AI Summary: OriGene products contributed to this study by providing the human TRPV4 Myc-DDK-tagged ORF expression plasmid (RC220160) and the pCMV6-Entry empty-vector control (PS100001), which were used for TRPV4 overexpression experiments in human aortic smooth muscle cells.
Snippet: For TRPV4 overexpression, cells were transfected with 2 µg per well of the human TRPV4 Myc-DDK-tagged ORF expression plasmid (OriGene Technologies, Rockville, MD, USA;RC220160;NM_021625), which is based on the pCMV6-Entry vector, or with the corresponding pCMV6-Entry empty-vector control (OriGene, PS100001)
Disruption of CTCF-mediated IGF2 repression promotes AKT-dependent mitochondrial oxidative stress and chondrocyte senescence in osteoarthritis
Zhang, Xu, Wu et al
Life Sci (2026) 405, 124689 DOI: 10.1016/j.lfs.2026.124689
AI Summary: OriGene's full-length human CTCF expression plasmid (catalog number PS100001) was used for CTCF overexpression experiments to investigate its role in regulating IGF2 expression and chondrocyte senescence in osteoarthritis.
Snippet: A full-length human CTCF expression plasmid (PS100001, OriGene, Rockville, MD, USA) was used for overexpression experiments. C28/I2 cells (2 × 105 per well in 6-well plates) were transfected with 4 μg of CTCF expression plasmid or empty vector using Lipofectamin
The Odf2 status contributes to asymmetric inheritance of CD133-positive recycling endosomes during cytokinesis in SK-N-DZ human neuroblastoma cells
Izumi, Mae, Tsukita et al
iScience (2026) 29 (8), 117062 DOI: 10.1016/j.isci.2026.117062
Cited by 1
AI Summary: In this study, the OriGene pCMV-Flag plasmid (Cat# PS100001) was used to facilitate molecular experiments investigating the role of Odf2 in the asymmetric inheritance of CD133-positive recycling endosomes during cell division in SK-N-DZ neuroblastoma cells.
Snippet: pCMV-Flag Origene Cat# PS100001 pcDNA ThermoFisher Scientific Cat#
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Showing 1-6 of 433 papers.
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