Carrier Free Antibodies
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OriGene offers carrier-free antibodies for over 2800+ targets. These are mouse monoclonal antibodies (TrueMAB, TrueRAB, & UltraMAB) in buffers free of carriers such as BSA, glycerol, and sodium-azide and are ready for downstream applications such as conjugations to fluorophores, metals, and enzymes.
Features and Benefits
- BSA, sodium-azide, and glycerol free for consistent conjugation
- Convenient: can be reconstituted in distilled water for the next steps
- Wide range to select from
- Over 13,000+ carrier-free antibodies against 2800+ targets
- Includes KO Validated and Mono-specific (ChiP-validated) antibodies
- Application validated: ICC, IHC, WB, IF, FC
- Compatible with a variety of labels, including fluorophores, metals, enzymes, and oligonucleotides
Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD3E clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, ) (1:300) Immunohistochemical staining of paraffin-embedded mouse colon tissue within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, ) (1:500) Immunohistochemical staining of paraffin-embedded human kidney tissue within the normal limits using anti-MSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, ) (1:1200)
Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD3E clone UMAB54 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500048) (1:300)
Immunohistochemical staining of paraffin-embedded mouse colon tissue within the normal limits using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM500006) (1:500)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800139) (1:1200)
Application Areas
OriGene carrier-free antibodies are ready-to-use for various technologies that use conjugated antibodies as the core building element:
- Fluorophore Conjugation: It has been widely used in many applications including fluorescence microscopy and flow cytometry. OriGene provides custom antibody conjugation services for various fluorophores and a conjugation kit for RPE.
- Enzyme Conjugation: HRP or AP conjugated antibodies are used in applications like IHC, ELISA, or Western blot. OriGene provides HRP conjugation kit and custom services for this purpose.
- Biotin Conjugation: The strong affinity of biotin-avidin is introduced in the downstream applications of the biotin-conjugated antibodies, which has been commonly used for the detection enhancement in Western blot, ELISA, or fluorescent microscopy. OriGene provides Biotin conjugation kit and custom service for antibody conjugation service.
- Oligonucleotide Conjugation: Multiplex IHCs using cyclic detection protocols conjugate a panel of antibodies with different coding oligonucleotides to obtain cellular spatial profiling data.
- Heavy Metal Conjugation: The combined technology of flow-cytometry and time-of-flight mass spectrometry (CyTOF) or Mass spectrometry imaging (MSI) uses heavy-metals and metal-chelating polymers to conjugate antibodies to resolve single-cell proteomics.
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