Lentivirus shRNA, plasmids and lentiviral particles
OriGene offers pre-made lentiviral shRNA plasmid kits containing 4 sequence-verified expression cassettes that target your gene of interest. Each lentiviral cassette contains three major functional elements: shRNA, a puromycin selection marker & a tGFP reporter. With a guaranteed knockdown of ≥70%, these kits and our ready-to-use lenti-shRNA particles are a great option for studying long-term gene knockdown.
Key Features and Benefits
- Potent shRNA design ( learn more )
- Genome-wide coverage: Human, Mouse & Rat
- Safety: 3rd generation system with improved biosafety
- Trusted vendor for lentiviral products: view citations
Figure 1: Preparation of lentiviral particles, HEK293T transfection (48 hours). Lenti vector only (right) vs Lenti-shRNA targeting tGFP (left).
A detailed look at lentiviral shRNA vector design
pGFP-C-shLenti vector
- Third generation Lentivector
- Chl marker for bacterial selection
- Three major functional elements within the 5’LTR and 3’LTR. All of them can be packaged to viral particles and transduced to many cell lines.
- shRNA expression cassette under U6 promoter
- Puromycin resistant for mammalian cell selection
- tGFP gene as reporter.
The shRNA expression cassette
- 29mer hairpin with a 7bp loop.
- U6 driven expression
- A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
- The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.
Customer Reviews
Recent Citations using our shRNA products
1 Neuronal ER-plasma membrane junctions couple excitation to Ca 2+ -activated PKA signaling Published in Nature Communications , using |
PubMed: 37633939 |
2 α 1 -Adrenergic receptor–PKC–Pyk2–Src signaling boosts L-type Ca 2+ channel Ca V 1.2 activity and long-term potentiation in rodents Published in eLife , using |
PubMed: 37338965 |
3 THOC2 expression and its impact on 5FU resistance in glioblastoma multiforme Published in American Journal of Cancer Research , using |
PubMed: 37424800 |
RNAi Resources |