lentivirus shrna, plasmids and lentiviral particles

  • Potent shRNA design
  • Genome-wide coverage: Human, Mouse & Rat
  • High transduction efficiency
  • 3rd Generation lentiviral vectors: Safer
  • Lentiviral particles offered

The Lentiviral cassette contains three major functional elements, shRNA, a puromycin selection marker & a tGFP reporter.

Details of the lentiviral shRNA vectors

pGFP-C-shLenti vector

  • Third generation Lentivector
  • Chloramphenicol marker for bacterial selection
  • Three major functional elements within the 5’LTR and 3’LTR. All of them can be packaged to viral particles and transduced to many cell lines.
    1. shRNA expression cassette under U6 promoter
    2. Puromycin resistant for mammalian cell selection
    3. tGFP gene as reporter.

The shRNA expression cassette

  • 29mer hairpin with a 7bp loop.
  • U6 driven expression
  • A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
  • The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.

OriGene also has another Lentiviral shRNA vector, pRFP-CB-shLenti

pGFP-C-shLenti vector map

Lenti Accessories

Lenti ORF Packaging Kit LentiTran Control Particles Concentrator Lenti Vectors Lenti Titer Kit Stabilizer

Here what the researchers are saying about OriGene’s lentiviral shRNA:

"Lenti Vector with GFP and selection marker makes easy to generate single cell clones."

"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."

"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."

"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals. The purchases from OriGene have been worth the price."

"I have tried lentivirus expressing shRNA and it was working to my expectation. I am very happy with the product."

"The ready-to-use lentivirus particles worked well in our hard-to-transduce cell lines."

"Great quality, good knock-down. Super satisfied."

"We used lentiviruses to silence Pmch gene and it worked beautifully."