Lentivirus shRNA, plasmids and lentiviral particles

OriGene offers pre-made lentiviral shRNA plasmid kits containing 4 sequence-verified expression cassettes that target your gene of interest. Each lentiviral cassette contains three major functional elements: shRNA, a puromycin selection marker & a tGFP reporter. With a guaranteed knockdown of ≥70%, these kits and our ready-to-use lenti-shRNA particles are a great option for studying long-term gene knockdown.

Key Features and Benefits

• Potent shRNA design ( learn more )
• Genome-wide coverage: Human, Mouse & Rat
• Safety: 3^rd generation system with improved biosafety
• Trusted vendor for lentiviral products: view citations

Figure 1: Preparation of lentiviral particles, HEK293T transfection (48 hours). Lenti vector only (right) vs Lenti-shRNA targeting tGFP (left).

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A detailed look at lentiviral shRNA vector design

pGFP-C-shLenti vector

• Third generation Lentivector
• Chloramphenicol marker for bacterial selection
• Three major functional elements within the 5’LTR and 3’LTR. All of them can be packaged to viral particles and transduced to many cell lines.
  1. shRNA expression cassette under U6 promoter
  2. Puromycin resistant for mammalian cell selection
  3. tGFP gene as reporter.

The shRNA expression cassette

• 29mer hairpin with a 7bp loop.
• U6 driven expression
• A Pol III termination sequence (TTTTTT) immediately downstream of the hairpin.
• The gene-specific shRNA cassette is sequence-verified to ensure its match to the target gene.

Lenti Accessories

Lenti ORF Packaging Kit LentiTran Control Particles Concentrator Lenti Vectors Lenti Titer Kit Stabilizer

Customer Reviews

"I have used lenti-ORF plasmids for the generation of overexpression stable cells. They work well. The cell selection marker in the vector made it easier to generate stable cells."

Review by A.J.

Medical University of South Carolina
 

"The ready-to-use lentivirus particles worked well in our hard-to- transduce cell lines."

Review by K.W.

University Hospital of Tuebingen, Children's Hospital

"I purchased a lenti-ORF from OriGene. I have good transfection and transduction efficiency with OriGene's lenti products. Overall, I am extremely satisfied."

Review by S.R.

Sidney Kimmel Cancer Center
 

"I used both the lentivirus and the lenti plasmids. Both of them worked very well. And the customer service is good too."

Review by B.S.

St. Jude Children's Research Hospital
 

"We Purchased multiple lenti vectors from OriGene and they all worked great."

Review by S.D.

MD Anderson Cancer Center
 

"Aml12 cells are very difficult to transfect using siRNAs. Hence, using OriGene's lenti-shRNA product helped tackle this issue."

Review by Y.L.

University of Queensland
 

"We as research labs always look for an efficient and reliable lentiviral products and OriGene has really done a great job for past several years for us. We are extremely satisfied with the product's efficacy of knockdown, literature provided and technical support when needed. On top of this the service was fast enough. I shall highly recommend researchers to go for OriGene. Thank you."

Review by A.G.

Moffitt Cancer Center
 

"Our lab tried to package our lentivirus using 293T cells but it was not that easy. OriGene's lentivirus provided us consistent results which made us have clear idea about the further research goals. The purchases from OriGene have been worth the price."

Review by H.T.

University of Arkansas for Medical Sciences

"I have tried lentivirus expressing shRNA and it was working to my expectation. I am very happy with the product."

Review by G.P.

Centre for Stem Cell Research
 

"We used lentiviruses to silence Pmch gene and it worked beautifully."

Review by M.F.

USP
 

"Great quality, good knock-down. Super satisfied."

Review by C.H.

Cambridge University
 

Recent Citations using our shRNA products

1. Neuronal ER-plasma membrane junctions couple excitation to Ca 2+ -activated PKA signaling Published in Nature Communications using TL708390	PubMed: 37633939
2. α 1 -Adrenergic receptor–PKC–Pyk2–Src signaling boosts L-type Ca 2+ channel Ca V 1.2 activity and long-term potentiation in rodents Published in eLife using TL710108	PubMed: 37338965
3. THOC2 expression and its impact on 5-fluorouracil resistance in glioblastoma multiforme Published in American Journal of Cancer Research using TL301111	PubMed: 37424800

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