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Stable Cell Lines

Comprehensive range of cell line products for cancer research and therapy development. Pre-made stable cancer cell lines with enhanced tracking and visualization capabilities.

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Stable Cell Lines for Reliable and Reproducible Research

OriGene provides a broad selection of high-quality stable cell lines designed to support reproducible research in cancer biology, functional genomics, and drug discovery. Our portfolio includes ready-to-use stable cell lines, reporter-labeled models, CRISPR-engineered cell lines, and cancer biomarker mutant lines, enabling researchers to accelerate experiments without the time and variability associated with transient expression systems.

All OriGene stable cell lines are developed using validated expression constructs and undergo quality control testing to ensure consistency, reliability, and ease of use across diverse experimental workflows.

Ready-to-Use Stable Cell Lines

OriGene’s pre-made stable cell lines are engineered for consistent gene expression and long-term stability across multiple passages. These models eliminate the need for in-house cell line development and are ideal for researchers who require assay-ready cell systems with minimal setup time.

Common applications include:

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Reporter Stable Cell Lines (GFP & Luciferase)

Stable cell lines expressing GFP or luciferase for sensitive, quantitative detection in imaging and screening assays.

  • Real-time monitoring of gene expression
  • Ideal for in vitro and in vivo imaging
  • Consistent reporter signal across passages
  • Suitable for high-throughput screening
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CRISPR/CAS9 Stable Cell Lines

Genetically engineered stable cell lines created using CRISPR/CAS9 for precise genome modification.

  • Gene knockout, knock-in, or functional disruption
  • Defined and stable genetic edits
  • Supports target validation and pathway analysis
  • Reduces variability vs transient editing
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CAR-T Validated Stable Cell Lines

Stable cell lines expressing CAR-T target antigens for reliable immune cell functional assays.

  • Validated antigen expression for CAR-T testing
  • Ideal for cytotoxicity and co-culture assays
  • Enables reproducible potency and specificity studies
  • Eliminates variability from transient expression
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Inducible Stable Cell Lines

Stable cell lines with regulated gene expression for controlled functional studies.

  • Licensed Tet-On® System: OriGene is licensed to use the Tet-On inducible expression system for the development of stable cell lines.
  • Tetracycline-inducible (Tet-On) systems
  • Temporal control of gene expression
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Cancer Biomarker Stable Cell Lines

Stable cell lines engineered with clinically relevant cancer biomarkers or mutations.

  • Models oncogenic pathways and drug response
  • Supports targeted therapy research
  • Genetically defined and consistent expression
  • Ideal for oncology screening applications

Case Studies: Over-expression in Stable Cell Lines

Validated results demonstrating successful gene over-expression in stable cell lines

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Target Gene X Over-expression
A human gene (Target X) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target X and β-actin antibody (TA811000) demonstrates over-expression of Target X in Clones 1, and at a higher level in Clone 2.
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Target Gene Y Over-expression
A human gene (Target Y) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target Y and β-actin antibody (TA811000) demonstrates over-expression of Target Y in the stable cell line.

Custom Stable Cell Line Development

In addition to off-the-shelf products, OriGene offers custom stable cell line development services tailored to your specific research needs. Options include constitutive or inducible expression, gene overexpression, gene knockdown, and CRISPR-based gene editing.

Custom services are supported by OriGene’s extensive ORF clone collection and expert technical support, delivering reliable cell models that align with your experimental objectives.