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Case Studies: Over-expression in Stable Cell Lines

A human gene (Target X) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target X and β-actin antibody (TA811000) demonstrates over-expression of Target X in Clones 1, and at a higher level in Clone 2.

A human gene (Target Y) was cloned into an expression vector (PS100024) and transfected into mammalian cells. Single colonies were established after 4 weeks of hygromycin drug selection. Western blot using a gene-specific antibody for Target Y and β-actin antibody (TA811000) demonstrates over-expression of Target Y in the stable cell line.

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