ProteanFect™
TuffCell Transfection Kit

Your solution for hard-to-transfect cells

A non-viral, non-liposomal, coacervate-based nucleic acid delivery platform based on mammalian endogenous protein, offering novel solution for efficient gene delivery.

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Quick-start Guide

New CRISPR Kit

85%+
Transfection Efficiency

Low
Cytotoxicity

High
Payload Capacity

Easy
Protocol

Case Studies

tt300007-hard-to-transfect-egfp-pdna-mrna — Figure 1: ProteanFect achieves high transfection efficiency with pDNA/mRNA in Jurkat T Cells

tt300007-primary-cells-sustained-gene-expression — Figure 2: ProteanFect achieves high transfection efficiency with mRNA in human primary T cells while maintaining an excellent safety profile

tt300007-primary-cells-72-hour-post-transfection — Figure 3: ProteanFect achieves high transfection efficiency with mRNA in NK cells

tt300007-primary-cells-high-efficiency-TRAC — Figure 4: ProteanFect enables high-efficiency gene editing in human primary T cells via co-delivery of Cas9 mRNA and sgRNA

tt300007-primary-cells-24-hour-post-transfection — Figure 5: ProteanFect achieves high transfection efficiency with mRNA in CD34+ hematopoietic stem cells without altering their differentiation

tt300007-hard-to-transfect-high-molecular-weight-pdna — Figure 6: ProteanFect achieves high transfection efficiency of high molecular weight nucleic acids or co-transfection of multiple nucleic acids in Jurkat T cells

tt300007-hard-to-transfect-egfp-untreated-after-transfection — Figure 7: ProteanFect achieves high transfection efficiency in K562 cells

tt300007-hard-to-transfect-gene-editing-tide — Figure 8: ProteanFect enables high-efficiency gene editing in Jurkat T Cells via co-delivery of Cas9 mRNA and sgRNA

tt300008-primary-mouse-immunocytes-24-hours-post-transfection — Figure 9: ProteanFect achieves high transfection efficiency with mRNA in mouse primary T cells while maintaining an excellent safety profile

tt300008-primary-mouse-immunocytes-nk1.1-gfp — Figure 10: ProteanFect achieves high tansfection efficiency with mRNA in mouse primary NK cells

tt300008-primary-mouse-immunocytes-high-effiecity-trac — Figure 11: ProteanFect enables high-efficiency gene editing in mouse primary T cells via co-delivery of Cas9 protein and sgRNA (RNP)

ProteanFect CRISPR RNP Transfection Kit vs. Lipofectamine CRISPRMAX

mRNA Load Comparison — Figure 13: Nano-Flow Cytometry Reveals Greater mRNA Cargo Density in Coacervates Than in Similar-Sized LNPs.

A coacervate is a type of droplet-like structure formed when charged polymers (such as proteins, peptides, or nucleic acids) spontaneously separate from solution into a dense, liquid-rich phase, surrounded by a more dilute outer phase. This phenomenon is called liquid-liquid phase separation (LLPS).

What is ProteanFect?

A groundbreaking nucleic acid delivery system that leverages coacervate technology to achieve efficient gene delivery through non-viral, non-electroporation, and non-liposomal approaches.

How ProteanFect Works

ProteanFect forms nanoparticles that rapidly deliver gene cargo into cells, enabling efficient gene expression and safe degradation after delivery.

Why Choose ProteanFect™ TuffCell?

Revolutionary coacervate-based delivery platform offering unprecedented efficiency and safety for hard-to-transfect cells.

Novel Technology

Solution for Hard-to-Transfect Cells

Unlock the full potential of your research with reliable gene delivery into the hardest cells — from T cells and NK cells to neurons and stem cells. ProteanFect™ delivers where lipids fail and electroporation harms.

Primary Cells

Gentle Transfection on Cells

Preserve cell health and function with a protein-based system that minimizes toxicity, stress, and membrane disruption — ensuring your immune cells kill, your neurons fire, and your stem cells differentiate as nature intended.

High Efficiency

Versatility of Cargo Type

From mRNA and siRNA to large plasmids and multiplex CRISPR components, ProteanFect™ flexibly delivers a broad range of nucleic acids — giving you one solution for every experiment.

Now Expanded for CRISPR Genome Editing

Introducing ProteanFect™ CRISPR RNP Transfection Kit — the newest addition to the ProteanFect family, purpose-built for efficient, non-viral delivery of CRISPR/Cas RNP into hard-to-transfect cells.

Explore Kits

Optimized for CRISPR Delivery

As CRISPR workflows move toward direct ribonucleoprotein delivery, researchers need transfection solutions that deliver high editing efficiency without sacrificing cell health. ProteanFect™ CRISPR RNP Transfection Kit is engineered specifically for Cas9 RNP delivery—enabling fast, scalable genome editing in primary, suspension, and other difficult cell types.

Direct RNP Delivery

Bypass plasmids and viral vectors with direct Cas9-gRNA complex delivery.

High Editing Efficiency

Optimized formulation for robust genome editing outcomes.

Low Toxicity

Coacervation-based delivery preserves viability in sensitive cells.

Faster CRISPR Workflow

Go from RNP assembly to edited cells in hours, not days.

Choose from our range of ProteanFect™ kits optimized for different cell types and applications.

KIT	TYPE	SPECIES / CELLS	SUPPORTED CARGO	BEST FOR
ProteanFect TuffCell Transfection Kit TT300007S / M / L	TuffCell	Human Mouse Rat Bovine +	mRNA siRNA pDNA	Broad-purpose delivery across many primary cell types and hard-to-transfect lines.
ProteanFect TuffCell Mouse Immunocyte Transfection Kit TT300008S / M / L	TuffCell	Mouse only T cells • NK cells • B cells	mRNA siRNA	mRNA delivery in mouse primary immune cells.
ProteanFect CRISPR RNP Transfection Kit TT300009S / M / L	CRISPR RNP	Human	Cas9 protein + sgRNA	Precise CRISPR gene editing.
ProteanFect CRISPR RNP Mouse Immunocyte Transfection Kit TT300010S / M / L	CRISPR RNP	Mouse only	Cas9 protein + sgRNA	CRISPR editing in mouse immune cells.

Available Product Kits

Choose from our range of ProteanFect™ kits optimized for different cell types and applications.

KIT	TYPE	SPECIES / CELLS	SUPPORTED CARGO	BEST FOR
ProteanFect TuffCell Transfection Kit TT300007S / M / L	TuffCell	Human Mouse Rat Bovine +	mRNA siRNA pDNA	Broad-purpose delivery across many primary cell types and hard-to-transfect lines.
ProteanFect TuffCell Mouse Immunocyte Transfection Kit TT300008S / M / L	TuffCell	Mouse only T cells • NK cells • B cells	mRNA siRNA	mRNA delivery in mouse primary immune cells.
ProteanFect CRISPR RNP Transfection Kit TT300009S / M / L	CRISPR RNP	Human	Cas9 protein + sgRNA	Precise CRISPR gene editing.
ProteanFect CRISPR RNP Mouse Immunocyte Transfection Kit TT300010S / M / L	CRISPR RNP	Mouse only	Cas9 protein + sgRNA	CRISPR editing in mouse immune cells.

Decision Guide

Find Your Protocol

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Recommended method

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ProteanFect™ TuffCell Transfection Kit

For Human Primary & Hard-to-Transfect Cells

A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.

✔ Pre‑qualified Cas9 protein with NLS for nuclear entry
✔ Optimized RNP formation buffer for maximum stability
✔ Compatible with synthetic & IVT sgRNAs
✔ Validated in T cells, NK cells, HSCs, iPSCs, and 50+ cell types
✔ Simple mix‑and‑add protocol — no electroporation required
✔ Scalable from 96‑well to larger format

Available Sizes

Preclinical

ProteanFect™ TuffCell Mouse Immunocyte Transfection Kit

For Mouse Primary Immune Cells

A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery in mouse primary immune cells.

✔ Formulation optimized for mouse immune cell biology
✔ High editing efficiency in mouse primary T & NK cells
✔ Maintains cell viability and immune function post‑edit
✔ Ideal for preclinical CAR‑T and immunotherapy models
✔ Same simple workflow as the human kit
✔ Compatible with mouse‑specific sgRNA designs

Available Sizes

New

ProteanFect™ CRISPR RNP Transfection Kit

For Human Primary & Hard-to-Transfect Cells

Engineered mammalian proteins optimized for delivering CRISPR RNP complexes (Cas9 protein and sgRNA) into primary and hard-to-transfect cells for gene editing.

✔ Pre‑qualified Cas9 protein with NLS for nuclear entry
✔ Optimized RNP formation buffer for maximum stability
✔ Compatible with synthetic & IVT sgRNAs
✔ Validated in T cells, NK cells, HSCs, iPSCs, and 50+ cell types
✔ Simple mix‑and‑add protocol — no electroporation required
✔ Scalable from 96‑well to larger format

Available Sizes

New

ProteanFect™ CRISPR RNP Mouse Immunocyte Transfection Kit

For Mouse Primary Immune Cells

Engineered mammalian proteins optimized for gene editing by efficiently transporting CRISPR RNP complexes (Cas9 protein and sgRNA) into primary mouse immune cells, including T, NK, and B cells.

✔ Formulation optimized for mouse immune cell biology
✔ High editing efficiency in mouse primary T & NK cells
✔ Maintains cell viability and immune function post‑edit
✔ Ideal for preclinical CAR‑T and immunotherapy models
✔ Same simple workflow as the human kit
✔ Compatible with mouse‑specific sgRNA designs

Available Sizes

Successfully Transfected Cell Types

Comprehensive list of cell lines and primary cells successfully transfected with ProteanFect™

Cell Type		Tested Cargo	Transfection Efficiency
Primary Cells	Human T Cells	mRNA siRNA RNP	50-100%
	Human B Cells	mRNA siRNA RNP	20-75%
	Human Natural Killer Cells	mRNA siRNA RNP	25-75%
	iPSCs (Induced Pluripotent Stem Cells)	pDNA mRNA siRNA RNP	50-100%
	HSCs (Human CD34+ Hematopoietic Stem Cells)	mRNA siRNA RNP	50-100%
	UC-MSCs (Umbilical Cord Mesenchymal Stem Cells)	mRNA siRNA	50-100%
	Human Skin Fibroblasts	pDNA mRNA siRNA	50-100%
	KSL (Mouse Hematopoietic Stem Cells)	mRNA siRNA	50-100%
	Mouse Neurons	pDNA mRNA siRNA	10-50%
	Mouse Oligodendrocytes	pDNA mRNA siRNA	50-100%
	Porcine Macrophages	mRNA siRNA	50-75%
	Bovine Fibroblasts	mRNA siRNA	50-100%
	M. rosenbergii Hemocytes	mRNA siRNA	25-75%
	Chicken Primordial Germ Cells	mRNA siRNA RNP	25-100%
	L. crocea Mesenchymal Stem Cells	mRNA siRNA	25-50%

Cell Type		Tested Cargo	Transfection Efficiency
Cell Lines	Jurkat (Human T Cells)	pDNA mRNA siRNA RNP	50-100%
	MOLT-16 (Human T-ALL Cells)	mRNA siRNA	10-30%
	Raji (Human B Cells)	mRNA siRNA	50-100%
	MEC-1 (Human B Cells)	mRNA siRNA	50-100%
	TMD8 (Human Diffuse Large B-Cell Lymphoma Cells)	mRNA siRNA	50-100%
	NK-92 (Human Natural Killer Cells)	mRNA siRNA	25-75%
	K562 (Human Chronic Myeloid Leukemia)	pDNA mRNA siRNA RNP	30-100%
	THP-1 (Human Monocytic Cells)	mRNA siRNA	50-100%
	Kasumi-1 (Human Acute Myeloid Leukemia)	mRNA siRNA RNP	50-100%
	MDS-L (Human Leukemia Cells)	mRNA siRNA	50-100%
	U937 (Human Myeloid Leukemia)	mRNA siRNA	50-75%
	HL-60 (Human Promyelocytic Leukemia)	mRNA siRNA	50-100%
	HFF (Human Fibroblasts)	mRNA siRNA RNP	50-100%
	LX-2 (Human Hepatic Stellate Cells)	pDNA mRNA siRNA	50-100%
	HepG2 (Human Liver Carcinoma)	pDNA mRNA siRNA RNP	25-75%
	KYSE-510 (Human Esophageal Squamous Cell Carcinoma)	mRNA siRNA	50-100%
	Mum2B (Human Melanoma)	mRNA siRNA	50-100%
	H1-hESC (Human Embryonic Stem Cells)	pDNA mRNA siRNA	50-100%
	U2OS (Human Osteosarcoma)	pDNA mRNA siRNA	50-100%
	HT-29 (Human Colorectal Adenocarcinoma Cells)	pDNA mRNA siRNA	50-100%
	5637 (Human Bladder Carcinoma Cells)	pDNA mRNA siRNA RNP	50-100%
	HGC-27 (Human Gastric Carcinoma Cells)	mRNA siRNA	50-100%
	SH-SY5Y (Human Neuroblastoma Cells)	pDNA mRNA siRNA	50-100%
	U251MG Cells (Human Glioblastoma Cells)	mRNA siRNA	50-100%
	HEK293 (Human Embryonic Kidney Cells)	pDNA mRNA siRNA RNP	50-100%
	Neuro 2A (Mouse Neuroblastoma Cells)	pDNA mRNA siRNA	50-100%
	MC38 (Mouse Colon Adenocarcinoma Cells)	mRNA siRNA	25-75%
	RAW264.7 (Mouse Macrophages)	mRNA siRNA	50-80%
	LLC (Mouse Lewis Lung Carcinoma)	pDNA mRNA siRNA	50-100%
	CH12 (Mouse Lymphoma Cells)	mRNA siRNA	50-100%
	BV-2 (Mouse Microglial Cells)	mRNA siRNA	25-50%
	C2C12 (Mouse Myoblasts)	pDNA mRNA siRNA	50-100%
	B16 (Mouse Melanoma Cells)	mRNA siRNA	25-75%
	MH-S (Mouse Macrophages)	mRNA siRNA	25-50%
	MODE-K (Mouse Intestinal Epithelial Cells)	mRNA siRNA	50-100%
	MEF (Mouse Embryonic Fibroblasts)	pDNA mRNA siRNA	25-100%
	3T3 (Mouse Embryonic Fibroblasts)	mRNA siRNA	25-50%
	OLN93 (Rat Oligodendrocyte Cells)	mRNA siRNA	50-100%
	MDBK (Bovine Kidney Epithelial Cells)	mRNA siRNA	50-100%
	MAC-T (Bovine Mammary Epithelial Cells)	mRNA siRNA	50-100%
	COS-7 (Monkey Kidney Fibroblast-like Cells)	pDNA mRNA siRNA	50-100%

View Full Primary Cell Lines Table

Transfection Protocol for mRNA per well of 96-well plate

How ProteanFect™ Compares

See how ProteanFect™ TuffCell Transfection Kit stands against other leading transfection methods in the market.

Transfection Method Comparison

Comprehensive comparison of ProteanFect™ TuffCell with other popular transfection methods.

Feature / Metric	ProTeanFect™ TuffCell	Lipid Reagents	Electroporation
Efficiency in Primary Cells	High (T cells, NK cells, neurons, stem cells, cardiomyocytes)	Low to inconsistent	High, but variable
Cell Viability	Excellent – maintains phenotype & function	Moderate – toxicity common	Poor – high cell death, stress
Suspension Cells (e.g., Jurkat, K562)	Suspension Cells (e.g., T cells and Jurkat cells); Reliable – mRNA/siRNA/pDNA ~50-90%	Very poor	Moderate – but with viability loss
Cargo Flexibility	Large plasmids, mRNA, siRNA, CRISPR components, multiplex delivery	Limited to smaller plasmids/RNA	Broad payloads, but limited by toxicity
Ease of Use / Workflow	Simple – mix & add, scalable to multi-well plates	Simple – mix & add	Complex setup, specialized device required
Functional Preservation	Preserves immune killing activity, stem cell differentiation, neuronal integrity	Often impairs cell health	Strongly impairs function due to stress
Scalability	High-throughput compatible	High-throughput compatible	Limited – batch processing only
Safety / Biocompatibility	Non-viral, protein-based, sulfate-free, biodegradable	Synthetic lipids, potential toxicity	Physical stress, not gentle
Best Use Case	Hard-to-transfect cells where viability matters most	Routine, easy-to-transfect adherent cell lines	Short-term high-efficiency delivery in difficult cells (sacrifice viability)

Proven Performance in the Hardest Cells

ProteanFect™ delivers high efficiency in primary and suspension cells — including T cells, NK cells, neurons, and stem cells — where lipids fail and electroporation harms viability.

Gentle Yet Powerful Delivery

Unlike harsh or toxic alternatives, ProteanFect™ maintains cell health, phenotype, and function, enabling more biologically relevant results.

Versatility Without Complexity

A simple, scalable mix-and-go system that supports a wide range of nucleic acid cargos (mRNA, siRNA, plasmids, CRISPR), making it the go-to solution for diverse applications.

Recent Citations for ProteanFect™

No.	Journal	DOI	Cell Type	Cargo type	Product SKU
1	Nature Communications	https://doi.org/10.1038/s41467-025-62976-4	Primary human CD8+ T cells	Cas9 mRNA and sgRNA	TT300007S
2	Nature Cell Biology	https://doi.org/10.1038/s41556-025-01753-3	Primary mouse CD8+ T cells	siRNA	TT300008S
3	Phytomedicine	https://doi.org/10.1016/j.phymed.2025.157649	Primary mouse CD8+ T cells	siRNA	TT300008S
4	Oncogenesis	https://doi.org/10.1038/s41389-026-00600-w	Primary mouse CD8+ T cells	siRNA	TT300008S
5	Nature Microbiology	https://doi.org/10.1038/s41564-025-02140-2	Primary mouse CD4+ Treg cells	siRNA	TT300008S
6	Signal Transduction and Targeted Therapy	https://doi.org/10.1038/s41392-025-02359-9	Primary mouse CD4+ T cells	Cas9 mRNA and sgRNA	TT300008S
7	Bioactive Materials	https://doi.org/10.1016/j.bioactmat.2026.01.030	Cord blood NK cells	siRNA	TT300007S
8	Nature Biotechnology	https://doi.org/10.1038/s41587-026-03007-5	Jurkat cells	RNP (Cas9 protein and sgRNA)	TT300009S
9	Science Advances	https://doi.org/10.1126/sciadv.adw7471	Jurkat cells	Plasmid	TT300007S
10	Journal of Advanced Research	https://doi.org/10.1016/j.jare.2025.11.018	Jurkat cells	Plasmid	TT300007S
11	Nature Genetics	https://doi.org/10.1038/s41588-025-02407-8	THP-1 cells	siRNA	TT300007S
12	Phytomedicine	https://doi.org/10.1016/j.phymed.2026.158382	Primary mouse CD8+ T cells	siRNA	TT300008S
13	Biochemical and Biophysical Research Communications	https://doi.org/10.1016/j.bbrc.2026.153918	Primary mouse T cells	mRNA	TT300008S
14	Nature Communications	https://doi.org/10.1038/s41467-026-74164-z	Jurkat cells	plasmid	TT300007S
15	Cancer Immunology, Immunotherapy	https://doi.org/10.1007/s00262-026-04387-y	Mouse ascitic CD19+ B cells	siRNA	TT300007S
16	Advanced Science	https://doi.org/10.1002/advs.202520693	Jurkat cells	plasmid	TT300007S
17	The Journal for ImmunoTherapy of Cancer	https://doi.org/10.1136/jitc-2025-014179	Primary human CD8+ T cells	plasmid	TT300007S
18	European Journal of Immunology	https://doi.org/10.1002/eji.70173	Primary mouse CD4+ T cells	Cas9 mRNA and sgRNA	TT300008S
19	Cell Reports	https://doi.org/10.1016/j.celrep.2026.117220	Primary mouse T cells/Primary human T cells	siRNA and mRNA	TT300008S / TT300007S
20	Food Science and Human Wellness	https://doi.org/10.26599/FSHW.2026.9251013	HEK293T	plasmid	TT300007S
21	Theranostics	https://doi.org/10.7150/thno.131793	Primary human Treg cells	Cas9 protein and sgRNA	TT300009S

Recent Citations for ProteanFect™

1 H2A.Z primes an epigenetic landscape for memory CD8+ T cell recall response Published in Nature Communications , using TT300007S	View Publication
2 CD160 dictates anti-PD-1 immunotherapy resistance by regulating CD8+ T cell exhaustion in colorectal cancer Published in Nature Cell Biology , using TT300008S	View Publication
3 Ginsenoside F3 alleviates T cell exhaustion via RIPOR2-mediated immunometabolic reprogramming to potentiate anti-PD-1 therapy Published in Phytomedicine , using TT300008S	View Publication
4 Fatty acid uptake mediated by FABP4 promotes the formation of CD8+T cell senescence through lipid peroxidation in the adipocyte-rich microenvironment of Ovarian Cancer Published in Oncogenesis , using TT300008S	View Publication
5 Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages Published in Nature Microbiology , using TT300008S	View Publication
6 High fructose consumption aggravates inflammation by promoting effector T cell generation via inducing metabolic reprogramming Published in Signal Transduction and Targeted Therapy , using TT300008S	View Publication
7 Cord blood natural killer cell-derived extracellular vesicles inhibit Zika virus infectivity through ITGB2/perforin-mediated envelope disruption in vitro and in vivo Published in Bioactive Materials , using TT300007S	View Publication
8 Single-round evolution of RNA aptamers with GRAPE-LM Published in Nature Biotechnology , using TT300009S	View Publication
9 Phosphorylation of shiftless is important for inhibiting the programmed −1 ribosomal frameshift Published in Science Advances , using TT300007S	View Publication
10 Single-nucleus RNA sequencing illuminates a functional analog of lymphoid organ in crustacean Published in Journal of Advanced Research , using TT300007S	View Publication
11 Spatially resolved multi-omics of human metabolic dysfunction-associated steatotic liver disease Published in Nature Genetics , using TT300007S	View Publication
12 Quercetin targets BCAT1 to potentiate CD8+ T cell immunity by rewiring branched-chain amino acid metabolism against colorectal cancer metastasis Published in Phytomedicine , using TT300008S	View Publication
13 A click-compatible BmTyr platform for biotin-free proximity labeling and subcellular proteome profiling in primary T cells Published in Biochemical and Biophysical Research Communications , using TT300008S	View Publication
14 Optimization of IS621 recombinase/bridge RNA-directed recombination for precise insertion of large DNA fragments in human cells Published in Nature Communications , using TT300007S	View Publication
15 Promoting APC function of B cells via reprogramming the fatty acid metabolism enhances anticancer immunity in metastatic ovarian cancer Published in Cancer Immunology, Immunotherapy , using TT300007S	View Publication
16 NUDT21 Drives T-Cell Acute Lymphoblastic Leukemia Through Dual Regulation of Alternative Polyadenylation and Transcriptional Activation Published in Advanced Science , using TT300007S	View Publication
17 Hyaluronic acid-CD44 signaling defines therapeutic resistance and immunosuppressive microenvironment in peritoneal metastasis of gastric cancer Published in The Journal for ImmunoTherapy of Cancer , using TT300007S	View Publication
18 Oleic Acid Promotes Treg Cell Differentiation via Autophagy Induction and Ameliorates DSS-Induced Colitis Published in European Journal of Immunology , using TT300008S	View Publication
19 Bach2 antagonizes leukotriene B4-Ltb4r1-JunB signaling to constrain Tfh13 cell differentiation Published in Cell Reports , using TT300008S, TT300007S	View Publication
20 Ganoderic Acid A Alleviates NAFLD by Facilitating PPARα-CPT1A Complex and Enhancing Gut Microbiota Diversity Published in Food Science and Human Wellness , using TT300007S	View Publication

See All Citations

Recent Citations for ProteanFect™

1 H2A.Z primes an epigenetic landscape for memory CD8+ T cell recall response Published in Nature Communications	PubMed: test
2 CD160 dictates anti-PD-1 immunotherapy resistance by regulating CD8+ T cell exhaustion in colorectal cancer Published in Nature Cell Biology	View Publication
3 High fructose consumption aggravates inflammation by promoting effector T cell generation via inducing metabolic reprogramming Published in Signal Transduction and Targeted Therapy	View Publication
4 Single-nucleus RNA sequencing illuminates a functional analog of lymphoid organ in crustacean Published in Journal of Advanced Research	View Publication
5 Mycobacterium tuberculosis-derived linoleic acid increases regulatory T cell function to promote bacterial survival within macrophages Published in Nature Microbiology	View Publication
6 Spatially resolved multi-omics of human metabolic dysfunction-associated steatotic liver disease Published in Nature Genetics	View Publication
7 Single-round evolution of RNA aptamers with GRAPE-LM Published in Nature Biotechnology	View Publication

See All Citations

Genomics

Proteomics

Genomics

Proteomics

ProteanFect™
TuffCell Transfection Kit

Your solution for hard-to-transfect cells

Case Studies

Key Performance Highlights of TT300007 in Jurkat T Cells

Key Performance Highlights of TT300007 in Human Primary T Cells

Key Performance Highlights of TT300007 in NK Cells

Key Performance Highlights of TT300007 in Human Primary T Cells

Key Performance Highlights of TT300007 in CD34+ Hematopoietic Stem Cells

Key Performance Highlights of TT300007 in Jurkat T cells

Key Performance Highlights of TT300007 in K562 Cells

Key Performance Highlights of TT300007 in Jurkat T Cells

Key Performance Highlights of TT300008 in Mouse Primary T Cells

Key Performance Highlights of TT300008 in Mouse Primary NK Cells

Key Performance Highlights of TT300008 in Mouse Primary T Cells

What is ProteanFect?

How ProteanFect Works

Why Choose ProteanFect™ TuffCell?

Now Expanded for CRISPR Genome Editing

Optimized for CRISPR Delivery

Available Product Kits

Find Your Protocol

ProteanFect™ TuffCell Transfection Kit

ProteanFect™ TuffCell Mouse Immunocyte Transfection Kit

ProteanFect™ CRISPR RNP Transfection Kit

ProteanFect™ CRISPR RNP Mouse Immunocyte Transfection Kit

Successfully Transfected Cell Types

Transfection Protocol for mRNA per well of 96-well plate

How ProteanFect™ Compares

Transfection Method Comparison

Recent Citations for ProteanFect™

Recent Citations for ProteanFect™

Recent Citations for ProteanFect™

Welcome to OriGene AI!

ProteanFect™TuffCell Transfection Kit

Your solution for hard-to-transfect cells

Case Studies

Key Performance Highlights of TT300007 in Jurkat T Cells

Key Performance Highlights of TT300007 in Human Primary T Cells

Key Performance Highlights of TT300007 in NK Cells

Key Performance Highlights of TT300007 in Human Primary T Cells

Key Performance Highlights of TT300007 in CD34+ Hematopoietic Stem Cells

Key Performance Highlights of TT300007 in Jurkat T cells

Key Performance Highlights of TT300007 in K562 Cells

Key Performance Highlights of TT300007 in Jurkat T Cells

Key Performance Highlights of TT300008 in Mouse Primary T Cells

Key Performance Highlights of TT300008 in Mouse Primary NK Cells

Key Performance Highlights of TT300008 in Mouse Primary T Cells

What is ProteanFect?

How ProteanFect Works

Why Choose ProteanFect™ TuffCell?

Now Expanded for CRISPR Genome Editing

Optimized for CRISPR Delivery

Available Product Kits

Find Your Protocol

ProteanFect™ TuffCell Transfection Kit

ProteanFect™ TuffCell Mouse Immunocyte Transfection Kit

ProteanFect™ CRISPR RNP Transfection Kit

ProteanFect™ CRISPR RNP Mouse Immunocyte Transfection Kit

Successfully Transfected Cell Types

Transfection Protocol for mRNA per well of 96-well plate

How ProteanFect™ Compares

Transfection Method Comparison

Recent Citations for ProteanFect™

Recent Citations for ProteanFect™

Recent Citations for ProteanFect™

Welcome to OriGene AI!

ProteanFect™
TuffCell Transfection Kit