Knockdown (KD) - Cell Lysates
Reproducibility relies on antibody specificity, as cross-reactivity with off-target proteins can lead to inconsistent and unreliable results. Our KD cell lysates are excellent tools for validating antibodies. Using shRNA KD lysates, researchers can confirm antibody reliability by detecting signals in wild-type (WT) cells that weaken or disappear in gene-silenced (KD) cells. This rigorous, data-driven approach ensures consistent and reproducible results for your research.
Our extensive catalog includes cell lysates prepared from C2C12, HEK293T, HeLa, HepG2, HT-1080, and MCF7 cell lines for over 500 targets.
Why Choose our Knockdown-Validated Cell Lysate?
- Knockdown Validation: Each lysate undergoes rigorous validation with RT-qPCR and Western blotting to ensure accurate mRNA or protein knockdown.
- All-in-One Solution: Each lysate product includes knockdown and wild-type lysates, eliminating the need to culture wild-type cells.
Discover the Data Behind the Validation
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IDH1 KD Cell Lysate |
EGFR KD Cell Lysate |
SOD2 KD Cell Lysate |
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WB analysis of IDH1 expression in WT and IDH1 KD HeLa cell lysates using anti-IDH1 antibody. Hsp90 serves as a loading control. |
WB analysis of EGFR expression in WT and EGFR KD HeLa cell lysates using anti-EGFR antibody. β-Tubulin serves as a loading control. |
WB analysis of SOD2 expression in WT and SOD2 KD HeLa cell lysates using anti-SOD2 antibody. β-Tubulin serves as a loading control. |
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RT-qPCR analysis of total RNA extracted from WT and IDH1 KD HeLa cells. |
RT-qPCR analysis of total RNA extracted from WT and EGFR KD HeLa cells. |
RT-qPCR analysis of total RNA extracted from WT and SOD2 KD HeLa cells. |
Share Your Knockdown Validation Data
Contribute your knockdown validation results using OriGene cell lysates through our "Write a Review" program. By sharing your review and data, you’ll qualify for our product review program and enjoy exclusive rewards. Learn more>>
| Recombinant Protein Resources |