MicroRNA Expression Plasmids

MicroRNAs (miRNAs) are naturally occurring non-coding RNA molecules (containing 21-23 nucleotides) that function in RNA silencing and post-transcriptional regulation of gene expression.

OriGene provides clones that facilitate over-expression of the miRNA of your choice. A region of ~600bp encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA, and cellular function can be analyzed.

Key Features

  • Genome-wide miRNA coverage (miRBase 20.0): 1829 human, 1160 mouse, 436 rat
  • GFP reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 10 μg transfection-ready DNA
  • 3' UTR reporter clones available for miRNA target validation
  • Cloning Vector: pCMV-MIR
MicroRNA Processing Diagram

Bulk Order Options

The complete set of the miRNA expression plasmids is available upon inquiry. Any purchase of over 10 miRNA plasmids is qualified for discount. Please contact techsupport@origene.com for more details.

Description List
Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes)
Human miRNA expression plasmid set (646 vectors, 10ug each in 2-D bar coded tubes)
Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates)
Human miRNA expression plasmid set (646 vectors, 2ug each in 96-well plates)

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