MicroRNA Expression Plasmids

MicroRNAs (miRNAs) are naturally occurring non-coding RNAs of 21-23 nt and they have been implicated in many biological processes and disease development. MiRNA are processed in multiple steps:

DNA→ Pri-miRNA → Pre-miRNA→ Mature miRNA

OriGene provides clones for over-expression of miRNA of your choice. A region of ~600bp region encompassing each miRNA is cloned downstream of a CMV promoter. Upon transfection, the cellular machinery will process the CMV-driven expression of miRNA precursor into mature miRNA and cellular function can be analyzed.

  • Genome wide miRNA coverage (miRBase release 20.0)
    - 1829 human, 1164 mouse and 436 rat
  • GFP as reporter for transfection monitoring
  • Neomycin selection marker for stable cell establishment
  • 10 μg transfection-ready DNA using PowerPrep® HP Kits
  • 3'-UTR reporter clones available for miRNA target validation
  • Cloning Vector: pCMV-MIR
MicroRNA Processing Diagram

Bulk Order Options

Catalog No. Description List
SC410001 Mouse miRNA expression plasmid set (486 vectors, 10ug each in 2-D bar coded tubes)
SC420001 Human miRNA expression plasmid set (646 vectors, 10ug each in 2-D bar coded tubes)
SC410002 Mouse miRNA expression plasmid set (486 vectors, 2ug each in 96-well plates)
SC420002 Human miRNA expression plasmid set (646 vectors, 2ug each in 96-well plates)

> 10 miRNA plasmids $250 each
> 30 miRNA plasmids $220 each
> 50 miRNA plasmids $200 each
> 100 miRNA plasmids $180 each
Please use “miBULK” as the quote number when place order.

Recent Citations

A New Polymorphism Biomarker rs629367 Associated with Increased Risk and Poor Survival of Gastric Cancer in Chinese by Up-Regulated miRNA-let-7a Expression, Xu, Q;Dong, Q;He, C;Liu, W;Sun, L;Liu, J;Xing, C;Li, X;Wang, B;Yuan, Y;PLoS ONE e95249 9 4. April 2014 [MIR-let-7a SKU# SC400707]

Association of a peptoid ligand with the apical loop of pri-miR-21 inhibits cleavage by Drosha, Diaz, JP;Chirayil, R;Chirayil, S;Tom, M;Head, KJ;Luebke, KJ; RNA, Feb 2014. [miR-16 SKU# SC400796]

Hepatitis C Virus Induced Changes in miRNA-107 and miRNA-449a Modulate CCL2 by Targeting IL6 Receptor Complex in Hepatitis, Sarma, NJ;Tiriveedhi, V;Crippin, JS;Chapman, WC;Mohanakumar, T; J. Virol., Jan 2014. [MIR-107 SKU# SC400023]