CRISPR Vectors
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OriGene offers a variety of CRISPR/Cas9 vectors for genome editing.
- All-in-One vectors: Contain both gRNA and Cas9 expression (lenti option available).
- T7 vectors: For in vitro production of gRNA and Cas9 mRNA
- Cas9 Nickase: Decrease off-targeting
- xCas9: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
| SKU | Name | Vector Type | Mammalian Selection | |
|---|---|---|---|---|
| All-in-One CRISPR/Cas9 vectors |
pCas-Guide | Non-viral | ||
| pCas-Guide-EF1a-GFP | Non-viral | |||
| pCas-Guide-EF1a-RFP | Non-viral | |||
| pCas-Guide-EF1a-CD4 | Non-viral | |||
| pLenti-Cas-Guide | Lentiviral | |||
| pLenti-EF1a-Cas-Guide | Lentiviral | |||
| pCas-Scramble | Non-viral | |||
| pCas-Scramble-EF1a-GFP | Non-viral | |||
| gRNA only vectors | pGuide | Non-viral | ||
| pGuide-EF1a-GFP | Non-viral | |||
| pLenti-Guide-Puro | Lentiviral | Puromycin | ||
| Cas9 only vectors | pAAVS1-Cas9-Puro-DNR | Non-viral | Puromycin | |
| pAAVS1-Cas9-BSD-DNR | Non-viral | Puromycin | ||
| pLenti-Cas9 | Lentiviral | |||
| pLenti-Cas9-IRES-Puro | Lentiviral | Puromycin | ||
| pLenti-EF1a-Cas9-IRES-Puro | Lentiviral | Puromycin | ||
| pLenti-Cas9-P2A-tGFP | Lentiviral | |||
| pLenti-Cas9-Myc-DDK-P2A-Puro | Lentiviral | Puromycin | ||
| pLenti-Cas9-mGFP-P2A-Puro | Lentiviral | Puromycin | ||
| CRISPR/Cas9 T7 vectors |
pT7-Guide-IVT | Non-viral | ||
| pT7-Cas9 | Non-viral | Neomycin | ||
| Cas9 Nickase | pCas-Guide-Nickase (H840A) | Non-viral | ||
| pCas-Guide-Nickase (D10A) | Non-viral | |||
| pT7-Cas9-Nickase (D10A) | Non-viral | Neomycin | ||
| Cas9 Dead Mutant | pCas-Guide-Nickase (D10A, H840A) | Non-viral | ||
| xCas9 Mutant | p-xCas9-Guide | Non-viral | ||
| Accessories | Annealing Buffer | Non-viral | Neomycin | |
| CF3 Primer | Non-viral | Neomycin | ||
| pCMV6-Entry-Cre | Non-viral | Neomycin |