CRISPR Vectors

OriGene offers a variety of CRISPR/Cas9 vectors for genome editing.

All-in-One vectors: Contain both gRNA and Cas9 expression (lenti option available).
T7 vectors: For in vitro production of gRNA and Cas9 mRNA
Cas9 Nickase: Decrease off-targeting
xCas9: Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

	SKU	Name	Vector Type	Mammalian Selection
All-in-One CRISPR/Cas9 vectors	GE100002	pCas-Guide	Non-viral
	GE100018	pCas-Guide-EF1a-GFP	Non-viral
	GE100140	pCas-Guide-EF1a-RFP	Non-viral
	GE100022	pCas-Guide-EF1a-CD4	Non-viral
	GE100010	pLenti-Cas-Guide	Lentiviral
	GE100045	pLenti-EF1a-Cas-Guide	Lentiviral
	GE100003	pCas-Scramble	Non-viral
	GE100021	pCas-Scramble-EF1a-GFP	Non-viral
gRNA only vectors	GE100042	pGuide	Non-viral
	GE100044	pGuide-EF1a-GFP	Non-viral
	GE100032	pLenti-Guide-Puro	Lentiviral	Puromycin
Cas9 only vectors	GE100037	pAAVS1-Cas9-Puro-DNR	Non-viral	Puromycin
	GE100039	pAAVS1-Cas9-BSD-DNR	Non-viral	Puromycin
	GE100028	pLenti-Cas9	Lentiviral
	GE100029	pLenti-Cas9-IRES-Puro	Lentiviral	Puromycin
	GE100030	pLenti-EF1a-Cas9-IRES-Puro	Lentiviral	Puromycin
	GE100031	pLenti-Cas9-P2A-tGFP	Lentiviral
	GE100075	pLenti-Cas9-Myc-DDK-P2A-Puro	Lentiviral	Puromycin
	GE100076	pLenti-Cas9-mGFP-P2A-Puro	Lentiviral	Puromycin
CRISPR/Cas9 T7 vectors	GE100025	pT7-Guide-IVT	Non-viral
CRISPR/Cas9 T7 vectors	GE100014	pT7-Cas9	Non-viral	Neomycin
Cas9 Nickase	GE100019	pCas-Guide-Nickase (H840A)	Non-viral
	GE100062	pCas-Guide-Nickase (D10A)	Non-viral
	GE100020	pT7-Cas9-Nickase (D10A)	Non-viral	Neomycin
Cas9 Dead Mutant	GE100061	pCas-Guide-Nickase (D10A, H840A)	Non-viral
xCas9 Mutant	GE100078	p-xCas9-Guide	Non-viral
Accessories	GE100007	Annealing Buffer	Non-viral	Neomycin
	GE100008	CF3 Primer	Non-viral	Neomycin
	GE100017	pCMV6-Entry-Cre	Non-viral	Neomycin

Vector Resources