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pCas-Guide-Dead Mutant (D10A, H840A)

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SKU
GE100061
Catalytically inactive Cas9 (D10A/H840A) vector for gRNA cloning (10 µg)

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Datasheet SDS Vector Sequence
$650.00
In Stock*
Size

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TP790151 Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli 500 pmol
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Specifications
Product Data
Function Cas9 Nickase
Features

All-in-one vector

  • Expresses human codon-optimized Cas9 D10A/H840A mutants, C-terminal Myc-DDK tagged
  • BamH I and BsmB I can be used for genomic target sequence cloning
  • Expresses gRNA containing the inserted target sequence and the gRNA scaffold
  • CF3 primer can be used to sequence the target sequence after cloning
Download Vector Sequence
OTI Disclaimer These products are manufactured and supplied by OriGene under license from ERS.
Storage Please store at -20°C for long term storage.
Stability The dried and the suspended DNA are stable for one year from date of shipping when stored at -20°C.
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.