Cas9 NC-NLS Streptococcus Pyogenes Recombinant Protein
SKU
TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
$150.00
In Stock*
Specifications
| Product Data | |
| Species | Streptococcus Pyogenes |
|---|---|
| Expression Host | E. coli |
| Expression cDNA Clone or AA Sequence | |
| Tag | N-His |
| Predicted MW | 165.0 kDa |
| Concentration | 10 uM |
| Purity | > 90% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 10 mM Tris-HCl, pH 7.4, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol |
| Bioactivity | In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease (Cat# TP790148). The input DNA is XmnI-linearized vector (PS100074) was cleaved by with a synthetic sgRNA. |
| Endotoxin | < 0.5 EU per 1 µg of the protein by the LAL |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -20°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Shipping | Dry Ice |
| Reference Data | |
Reviews
Documents
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
