CRISPR RNP Delivery Platform
ProteanFect™ CRISPR RNP
Transfection Kits
A coacervate-based, protein-engineered delivery system that gets Cas9 RNP complexes into the primary and hard-to-transfect cells that matter — with no electroporator, no virus, and no lipid-driven toxicity.
Why ProteanFect
Built for the cells conventional transfection can't reach
Lipofection stresses primary cells. Electroporation demands dedicated hardware and a BSL-2 upgrade. ProteanFect's engineered-protein coacervate mechanism was designed to solve both problems at once.
High efficiency in cells that matter
Up to 88.9% mRNA transfection in primary human T cells, and 83% indel efficiency in primary mouse T cells — validated data, not estimates.
No specialized equipment
Works with standard cell culture equipment and serum-free media. No electroporator, no viral production facility, no BSL-2 upgrade required.
Cell viability preserved
The gentle coacervate mechanism maintains cell health. Viability is typically restored by day 2 — cells remain functional for flow cytometry, sequencing, and adoptive transfer.
Ordering Information
Kit sizes, contents & pricing
All kits ship on dry ice and include ready-to-use positive controls (EGFP mRNA, Cas9 protein, and target-specific sgRNA).
ProteanFect™ CRISPR RNP Transfection Kit — human & mammalian primary cells / cell lines
| Cat. No. | Size | Kit Contents | List Price (USD) | Order |
|---|---|---|---|---|
| 0.03 mL | 6 vials — Reagent A (1.2 mL), Reagent B (0.03 mL), Reagent C (0.5 mL), EGFP mRNA (5 µL), Cas9 protein (25 µL), Human TRAC sgRNA (5 µL) | $389 | ||
| 0.15 mL | 7 vials — Reagent A (6.0 mL), Reagent B (0.15 mL), Reagent C (1.2 mL ×2), EGFP mRNA (5 µL), Cas9 protein (120 µL), Human TRAC sgRNA (5 µL) | $1,539 | ||
| 1.5 mL | 34 vials — Reagent A (6.0 mL ×10), Reagent B (0.15 mL ×10), Reagent C (10 mL ×2), EGFP mRNA (5 µL), Cas9 protein (120 µL ×10), Human TRAC sgRNA (5 µL) | $8,839 |
ProteanFect™ CRISPR RNP Mouse Immunocyte Transfection Kit — primary mouse T, NK & B cells
| Cat. No. | Size | Kit Contents | List Price (USD) | Order |
|---|---|---|---|---|
| 0.03 mL | 6 vials — Reagent A (1.2 mL), Reagent B (0.03 mL), Reagent C (0.3 mL), EGFP mRNA (5 µL), Cas9 protein (25 µL), Mouse Trac sgRNA (5 µL) | $399 | ||
| 0.15 mL | 6 vials — Reagent A (6.0 mL), Reagent B (0.15 mL), Reagent C (1.5 mL), EGFP mRNA (5 µL), Cas9 protein (120 µL), Mouse Trac sgRNA (5 µL) | $1,629 | ||
| 1.5 mL | 34 vials — Reagent A (6.0 mL ×10), Reagent B (0.15 mL ×10), Reagent C (8.0 mL ×2), EGFP mRNA (5 µL), Cas9 protein (120 µL ×10), Mouse Trac sgRNA (5 µL) | $8,999 |
Pricing shown is list price in USD and subject to change. Contact sales@origene.com for institutional, volume, or distributor pricing.
The Technology
Coacervate-based RNP delivery mechanism
Engineered mammalian proteins (Reagents A, B, C) encapsulate Cas9 RNP into a stable coacervate complex, deliver it into the cytosol, and step out of the way.
Coacervate Formation
Engineered mammalian proteins (Reagents A, B, C) encapsulate the RNP into a stable coacervate complex.
Cellular Uptake
Coacervates undergo efficient cellular internalization, followed by robust cytosolic release of bioactive cargo.
Genome Editing
Cas9 edits the target locus, then degrades — limiting off-target nuclease activity versus plasmid or viral expression.
Validated Performance
TRAC knockout in primary T cells, quantified two ways
Positive-control sgRNAs targeting the TRAC/Trac locus were used to benchmark editing at 72 hours post-transfection by flow cytometry (CD3–TCR protein loss) and TIDE analysis of Sanger sequencing (genomic indel rate).
Human T cells (TT300009)
Target: human TRAC · Detection: 72h post-transfection
Mouse T cells (TT300010)
Target: mouse Trac · Detection: 72h post-transfection
Simplified Workflow
Complex to result in under 3 hours
Simplified transfection protocol shown per well of a 96-well plate. Scales linearly to 48-, 24-, 12-, and 6-well formats.
Complex Preparation
Mix 0.8 µg Cas9 + 0.3 µg sgRNA with 40 µL Reagent A. Add Reagent B, mix thoroughly, then add Reagent C and place on ice. Use within 30 minutes.
Cell Preparation
Centrifuge cells at 300 g / 5 min, wash with Opti-MEM, and resuspend serum-free at the recommended density for your format.
Transfection
Mix complex with cell suspension. Incubate 15–30 minutes in a standard cell culture incubator — no electroporator required.
Culture & Assess
Add complete medium and culture. Assess editing efficiency by flow cytometry or TIDE at 48–72 hours.
Applications
Where researchers put ProteanFect to work
Resources
Documentation & downloads
User Manual — CRISPR RNP Transfection Kit
Full protocol, storage conditions, plate-format scaling tables, and troubleshooting guide.
Download HereUser Manual — Mouse Immunocyte Kit
Species-optimized protocol for mouse T, NK, and B cells, plus application data.
Download HereFAQ
Common questions
Ready to see coacervate delivery in your own cells?
Talk to our applications team about which ProteanFect kit fits your model system, or request a quote for your lab.
