Over-Expression Cell Lysates

Comprehensive library of whole-cell lysates from HEK293T cells over‑expressing target proteins. Validated positive controls for Western Blot and protein research applications.

Western Blot Controls

Positive Controls for Western Blot

OriGene's over-expression lysates are often cited for use as control in peer-reviewed articles.

Confirm Antibody Specificity with Confidence

Positive control in Western Blot: The specificity of a given antibody to CD2 is unknown. The antibody detects endogenous expression of a protein in Jurkat cells but not in other cell lines, raising the non-specificity concern of the antibody.

Adding a known positive CD2 over-expression cell lysate control along with a negative untransfected cell lysate confirms the CD2 detection.

Validates every stage of your Western blot workflow

 Confirms antibody specificity against target protein

 Includes negative untransfected lysate control

Pre-validated by Western Blot before shipping

Qualitative Antibody Evaluation

Five commercial antibodies against human P53 evaluated in Western blot experiments with P53 over‑expression cell lysate.

Lysate was serially diluted before SDS‑PAGE and immunoblotting. Antibody quality and star rating is based on P53 protein detection level.

Protein Arrays for Large‑Scale Screening

OriGene’s high‑density protein array is made with more than 10,000 unique over‑expression lysates, printed in duplicate, with controls.

Such protein arrays can be used in antibody specificity validation, protein‑protein interaction, and auto‑antibody screening studies.