With a collection of over 100,000 primary antibodies and commonly used secondary and tag antibodies, OriGene offers an extensive range of antibodies from various species. We use advanced technologies to generate better antigens and validate the antibodies through multiple applications and protein samples. Our premium antibody brands include TrueMAB^®, UltraMAB^®, and TrueRAB^™.

Specificity is one of the most important attributes of antibodies. At OriGene, we have been working hard to validate specificities of our antibodies.  

Figure 1. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using human Her2 overexpression cell lysates and endogenous cell line lysates. UM500036 can recognize the specific Her2 protein at predicted size (~140KDa) in overexpression cell ysate (lane 1 ) and endogenous lysates prepared from Hela, A549 and MCF7 cell lines. The specificity is further proved by using an independent antibody (anti-DDK antibody in the middle) against the same recombinant overexpressed Her2 protein.

Figure 2. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using 10k protein chip technology. UM500036 shows no cross-reactivity with other protein on the tested protein microarray containing >10,000 overexpression lysates for different human proteins.

Figure 3. Specificity validation of anti-Her2 antibody (Cat. No. UM500036) using Her2 knockout cell lysate. UM500036 recognizes the specific Her2 protein at correct size in wild type Hela cell lysate (WT lane), and shows cross-reactivity with any other protein in the double knockout cell lysates (KO lane).
WT: parental wild type Hela cell lysate
KO: Her2 double knockout Hela cell lysate