GBI Labs provides high quality chromogenic detection kits to detect the expression of target proteins in tissues. Chromogenic detection uses enzymes to convert soluble substrates to insoluble, chromogenic end products at the site of antigen expression. The two most common enzymes used for chromogenic detection are Horseradish peroxidase (HRP) and alkaline phosphatase (AP), which can convert 3,3' diaminobenzidine (DAB) and 3-amino-9-ethylcarbazole (AEC), into brown and red end products, respectively.
GBI Labs offers many different types of IHC detection kits. They are either Biotin-Streptavidin based and Polymer-based detection systems. Biotin-Streptavidin kits use biotinylated secondary antibody and HRP or AP labeled-streptavidin to detect primary antibody. Polymer-based detection method use polymer to link a large number of antibodies and enzymes, that normally has better sensitivity, and easier process comparing to the biotin based system.
In addition to different amplification methods, GBI IHC detection kits contain many different 2nd antibodies that can be used for primary antibodies from different species such as mouse, rabbit, goat, rat, chicken, sheep, Armenian hamster, and guinea pig. All secondary antibodies used in our detection system are highly purified antibodies.
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