CRISPR/Cas9 Gene Editing Cloning Services


CRISPR/Cas9, is an RNA-guided genome editing tool which allows researchers to do gene knockout, knock-in SNPs, insertions and deletions in cell lines and animals. OriGene offers CRISPR/Cas9 gene editing cloning services, including gRNA cloning and donor template construction.

Custom gRNA cloning service

OriGene’s all in one CRISPR/Cas vectors and T7 vector system can serve your genome editing needs. To provide convenience to you, we offer target sequence cloning service into any of our CRISPR/Cas vectors. This service has the following benefits:

  • Cost-effective
  • Fast, 7-10 days
  • Two options, OriGene’s target sequence design or self design
  • Self-design: Customers provide the target sequences, choose a vector and OriGene constructs the plasmid.

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  • OriGene-design: Customers provide around 100 bp genome sequence and OriGene designs the target sequences using our proprietary gRNA design tool.

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Be sure to click on the "Submit" button, then save the quote. When placing an order, please refer to the catalog number on the request form

Custom Donor Vector Construction

  • Donor vector construction with a predesigned cassette
    • GFP-LoxP-PGK-Puro-LoxP
    • RFP-LoxP-PGK-BSD-LoxP
    • Luc-LoxP-PGK-Puro-LoxP
    • mBFP-LoxP-PGK-Neo-LoxP
  • Ability to design your own vector

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Cre expression vector for Cre-LoxP recombination