Seamless Cloning & Assembly Master Mix (2X)

The Seamless Cloning & Assembly Master Mix (2X) is a simple, fast, and efficient DNA seamless cloning method that connects multiple overlapping DNA fragments into a joined DNA molecule without the use of restriction enzyme cloning. The buffer components and enzymes in the 2X Master Mix are optimized to enhance the efficiency and robustness of assembly reaction.

Key Features and Benefits

  • Assembly of up to 5 inserts
  • Final product is transformation-ready
  • No restriction enzyme digestions
  • No extra sequences introduced
Procedure Overview
Procedure Details
SKU Description Size
NP100054 The Seamless Cloning & Assembly Master Mix can clone up to 5 inserts into any vector in a targeted manner. 100 ul