Knockdown (KD) - Validated Primary Antibodies
We ensure the highest quality antibodies by validating their specificity through knockdown methods. Using shRNA KD, we confirm an antibody’s reliability by testing its ability to detect signals in wild-type (WT) cells, which disappear or weaken significantly in gene-silenced (KD) cells. This data-driven approach guarantees confidence in your results.
Our extensive catalog includes primary antibodies for diverse targets, tested across applications like Western blotting, ICC/IHC, and flow cytometry. Most antibodies are rabbit monoclonal with reactivity toward humans, mice, and rat targets.
Why Choose shRNA Knockdown Validation?
- Offers stable, effective gene silencing via lentiviral integration.
- Ideal for nondividing and hard-to-target cells.
- Retains gene functionality, crucial for studying essential genes
Discover the Data Behind the Validation
KD-Validated Anti-IDH1 Rabbit Monoclonal Ab |
KD-Validated Anti-EGFR Rabbit Monoclonal Ab |
KD-Validated Anti-UCHL1 Rabbit Monoclonal Ab |
---|---|---|
![]() WB analysis of IDH1 expression in WT and IDH1 KD HeLa cell lysates using anti-IDH1 antibody. Hsp90 serves as a loading control |
![]() WB analysis of EGFR expression in WT and EGFR KD HeLa cell lysates using anti-EGFR antibody. β-Tubulin serves as a loading control. |
![]() WB analysis of UCHL1 expression in WT and UCHL1 KD HAP1 cell lysates using anti-UCHL1 antibody. β-Tubulin serves as a loading control. |
![]() Flow cytometric analysis of IDH1 expression in C2C12 cell using anti-IDH1 antibody. Green: isotype control; red: IDH1 |
![]() Immunocytochemical staining of HepG2 cells with IDH1 antibody. Nuclei: stained blue with DAPI; EGFR: stained magenta with Alexa Fluor® 647 |
![]() Immunocytochemical staining of HAP-1 cells with UCHL1 antibody. Nuclei: stained blue with DAPI; UCHL1: stained magenta with Alexa Fluor® 647 |
Related Products
Share Your Knockdown Validation Data
Contribute your knockdown validation results using OriGene cell lysates through our "Write a Review" program. By sharing your review and data, you’ll qualify for our product review program and enjoy exclusive rewards. Learn more>>
Antibody Resources |