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Cell Transfection Reagents

Featured: ProteanFect™ Products Now Available Featured: EZ-LNP™ Products Now Available

Researchers need to do gene overexpression (cDNA clones), gene knockdown (siRNA or shRNA) and gene knockout (CRISPR) to study gene functions. Effectively deliver those gene related products into cells is critical for their research. OriGene provides a broad range of transfection reagents for all of these different gene formats.

New! ProteanFect™ TuffCell Transfection Kit

Your solution for efficient gene delivery in tough-to-transfect primary cells. Based on coacervate technology using engineered mammalian proteins, this non-viral, non-liposomal, and non-electroporation solution ensures efficient and robust delivery of a comprehensive range of nucleic acids, including plasmid DNA, mRNA, siRNA, and CRISPR tools.

Selection Guide

Application	Reagent	Features	Action
DNA/RNA/siRNA/RNP NEW	ProteanFect™	A non-viral, non-liposomal, non-electroporation platform built on coacervate technology using engineered mammalian proteins. Specifically designed for hard-to-transfect primary cells including T cells, NK cells, and monocytes. Superior delivery of RNA into a wide range of primary cells and difficult to transfect cell lines. Achieves 85%+ transfection efficiency while maintaining excellent safety profile.	View Details
RNA Delivery NEW	EZ-LNP™	Instrument-free protocol - mix & go in under 1 minute High efficiency - up to 10x protein expression Versatile - from In vitro to In vivo Low inflammation & injection-site pain	View Details
Plasmid DNA	TurboFectin 8.0™	Superior performance, comparable with FuGene® 6 Broad spectrum for commonly used established cell lines, such as Hela, HEK293, MCF, 3T3, COS7, A549, etc.	View Details
Plasmid DNA	MegaTran™	Economical choice Excellent transfection efficiency Broad cell spectrum (MegaTran 2.0)	View Details
Plasmid DNA	293Tran™	Formulated specially for HEK293 transfection High transfection efficiency Low cytotoxicity	View Details
siRNA	siTran™	High efficiency and low cytotoxicity Commonly used cell lines and primary cells, such as human primary T cells	View Details
Lentivirus Production	LentiTran™	Exceptional high titer lentivirus production Simple & robust transfection procedure Very affordable	View Details

ProteanFect™ TuffCell Transfection Kit

Your solution for tough-to-transfect cells.

Why Choose ProteanFect™?

High efficiency
Achieve up to 85%+ Transfection Efficiency
High Payload Capacity
Deliver thousands of nucleic acids simultaneously
Cell Compatibility & Safety
Excellent safety and biocompatibility

Complete Kit Includes:

Reagents
EGFP mRNA and pDNA
User Manual

ProteanFect™ Products

SKU	Name	Description
TT300007S	ProteanFect TuffCell Transfection Kit - 0.02 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
TT300007M	ProteanFect TuffCell Transfection Kit - 0.1 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
TT300007L	ProteanFect TuffCell Transfection Kit - 1.0 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery into a wide range of primary cells and difficult to transfect cell lines such as immune cells, stem cells, and neuronal cells.
TT300008S	ProteanFect TuffCell Mouse Immunocyte Transfection Kit - 0.02 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery in mouse primary immune cells
TT300008M	ProteanFect TuffCell Mouse Immunocyte Transfection Kit - 0.1 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery in mouse primary immune cells
TT300008L	ProteanFect TuffCell Mouse Immunocyte Transfection Kit - 1.0 mL	A non-viral, non-electroporation, non-liposomal transfection kit using engineered mammalian proteins, tailored for high-efficiency delivery in mouse primary immune cells

EZ-LNP™ Transfection Kit

Simplifying RNA delivery with instrument-free LNP protocol

Why Choose EZ-LNP™?

Instrument-free protocol
Mix & go in under 1 minute
High efficiency
Achieve up to 10x protein expression
Transferable results
From In vitro to In vivo
Low inflammation
Reduced injection-site pain

Complete Kit Includes:

EZ-LNP reagent
RNA buffer
User Manual

EZ-LNP™ Products

SKU	Name	Description
TT300004S	EZ-LNP RNA Transfection Kit - 0.3 mL	A user-friendly, instrument-free LNP kit enabling effective RNA transfection in vitro and RNA delivery in vivo.
TT300004L	EZ-LNP RNA Transfection Kit - 1.0 mL	A user-friendly, instrument-free LNP kit enabling effective RNA transfection in vitro and RNA delivery in vivo.
TT300005	EZ-LNP Liver Delivery Kit - 1.0 mL	A user-friendly, instrument-free LNP kit supporting efficient targeted delivery to liver cells for in vivo applications.
TT300006	EZ-LNP Spleen Delivery Kit - 1.0 mL	A user-friendly, instrument-free LNP kit supporting efficient targeted delivery to spleen cells for in vivo applications.