Secondary Antibodies

Secondary antibodies are normally used in pair with primary antibodies to detect, sort or purify target proteins. To do this, a secondary antibody must bind to the primary antibody and have specificity for the host and isotype of the primary antibody being used.

Many secondary antibodies are conjugated antibodies, which can be used in various immunoassays, such as ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC) and Flow Cytometry (FC). Selection of a secondary antibody is very straightforward if you know the class of the primary antibody, the source host, and downstream application. For example, if you are looking for a secondary antibody to detect a mouse monoclonal primary antibody in WB, you should choose a HRP conjugated anti-mouse secondary antibody. If you are doing immunofluorescence or flow cytometry, you might want to use a secondary antibody labeled with a fluorescent reporter.

OriGene offers more than 70 high quality secondary antibodies against different species with various conjugations. Browse the following list to find out the right secondary antibody for your research.